1999
Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis.
Magnarelli L, Van Andel A, Ijdo J, Heimer R, Fikrig E. Serologic testing of horses for granulocytic ehrlichiosis, using indirect fluorescent antibody staining and immunoblot analysis. American Journal Of Veterinary Research 1999, 60: 631-5. PMID: 10328436, DOI: 10.2460/ajvr.1999.60.05.631.Peer-Reviewed Original ResearchConceptsIndirect fluorescent antibodyNCH-1 strainPolymerase chain reactionGranulocytic ehrlichiosisSeropositivity rateSerum samplesBlood samplesBorrelia burgdorferi erpT expression in the arthropod vector and murine host
Fikrig E, Chen M, Barthold S, Anguita J, Feng W, Telford S, Flavell R. Borrelia burgdorferi erpT expression in the arthropod vector and murine host. Molecular Microbiology 1999, 31: 281-290. PMID: 9987129, DOI: 10.1046/j.1365-2958.1999.01171.x.Peer-Reviewed Original ResearchConceptsRNA polymerase chain reactionPolymerase chain reactionB. burgdorferi-infected miceBurgdorferi-infected miceB. burgdorferiMurine infectionChain reactionMurine hostImmunization of miceExtracutaneous sitesDifferential antibodyPersistent infectionArthropod vectorsIndirect immunofluorescenceBSK II mediumMiceImmunofluorescence studiesInfectionBurgdorferiAntibodiesAntigenBorrelia burgdorferi genesSmall minorityMammalian hostsNorthern blot
1991
Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent
Berland R, Fikrig E, Rahn D, Hardin J, Flavell RA. Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent. Infection And Immunity 1991, 59: 3531-3535. PMID: 1894359, PMCID: PMC258917, DOI: 10.1128/iai.59.10.3531-3535.1991.Peer-Reviewed Original ResearchConceptsLyme diseaseHumoral responseLate-stage Lyme diseaseEarly humoral responseBorrelia burgdorferiFlagellar antigensPolymerase chain reactionSerological testingImmunodominant domainWestern blotPatientsLyme disease agentAntigenDiseaseChain reactionGlutathione S-transferaseFusion proteinAffinity-purified fusion proteinFlagellin fragmentBacterial flagellinSerumBurgdorferiS-transferaseDisease agentsMolecular characterization