2023
A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria
Chuang Y, Alameh M, Abouneameh S, Raduwan H, Ledizet M, Weissman D, Fikrig E. A mosquito AgTRIO mRNA vaccine contributes to immunity against malaria. Npj Vaccines 2023, 8: 88. PMID: 37286568, PMCID: PMC10244833, DOI: 10.1038/s41541-023-00679-x.Peer-Reviewed Original ResearchHumoral responseFuture malaria vaccinesMosquito saliva proteinsRobust humoral responseImmunization of miceVector antigensIsotype antibodiesIgG titersImmunized miceMalaria vaccinePassive immunizationIgG2a isotypesMRNA vaccinesPlasmodium infectionMosquito bitesMRNA lipid nanoparticlesVaccineMRNA-LNPPlasmodium sporozoitesMalariaMiceImmunizationSaliva proteinsPotential usefulnessVertebrate hosts
2021
A Mosquito AgTRIO Monoclonal Antibody Reduces Early Plasmodium Infection of Mice
Chuang YM, Tang XD, Fikrig E. A Mosquito AgTRIO Monoclonal Antibody Reduces Early Plasmodium Infection of Mice. Infection And Immunity 2021, 90: e00359-21. PMID: 34724388, PMCID: PMC8788779, DOI: 10.1128/iai.00359-21.Peer-Reviewed Original ResearchConceptsMonoclonal antibodiesFuture malaria vaccinesInfection of miceIsotype monoclonal antibodyVector antigensProtective immunityPassive immunizationMalaria vaccinePlasmodium infectionPassive transferProtein monoclonal antibodySignificant protectionSynergistic protectionMiceInfectionAntibodiesFc regionAntiserumVertebrate hostsProtein TrioImmunizationVaccineMalariaAntigenImmunityAgBR1 and NeSt1 antisera protect mice from Aedes aegypti-borne Zika infection
Marin-Lopez A, Wang Y, Jiang J, Ledizet M, Fikrig E. AgBR1 and NeSt1 antisera protect mice from Aedes aegypti-borne Zika infection. Vaccine 2021, 39: 1675-1679. PMID: 33622591, PMCID: PMC7990057, DOI: 10.1016/j.vaccine.2021.01.072.Peer-Reviewed Original ResearchConceptsMosquito saliva proteinsPassive immunizationZIKV infectionEarly host responseDiverse clinical symptomsViral burdenSaliva proteinsClinical symptomsVaccine strategiesZika infectionHost responseModel antigenInfectionPartial protectionMiceImmunizationZIKVAntiserumIndividual antiseraAgBR1ViremiaSymptomsAntigenBloodAntibodies
2020
The Effects of A Mosquito Salivary Protein on Sporozoite Traversal of Host Cells
Chuang YM, Agunbiade TA, Tang XD, Freudzon M, Almeras L, Fikrig E. The Effects of A Mosquito Salivary Protein on Sporozoite Traversal of Host Cells. The Journal Of Infectious Diseases 2020, 224: 544-553. PMID: 33306099, PMCID: PMC8328219, DOI: 10.1093/infdis/jiaa759.Peer-Reviewed Original ResearchConceptsPassive immunizationCell traversal activityMosquito saliva proteinsMosquito salivary proteinsMosquito salivaNeutrophil chemotaxisProtein monoclonal antibodyProtective effectSporozoite glidingSporozoite infectivitySporozoite traversalMonoclonal antibodiesHost skinAnopheles mosquitoesProtein 1ImmunizationBlood mealSporozoitesSalivaMiceHost cellsSaliva proteinsSalivary proteinsAntiserumVertebrate hostsVaccination with Aedes aegypti AgBR1 Delays Lethal Mosquito-Borne Zika Virus Infection in Mice
Wang Y, Marin-Lopez A, Jiang J, Ledizet M, Fikrig E. Vaccination with Aedes aegypti AgBR1 Delays Lethal Mosquito-Borne Zika Virus Infection in Mice. Vaccines 2020, 8: 145. PMID: 32218189, PMCID: PMC7348886, DOI: 10.3390/vaccines8020145.Peer-Reviewed Original ResearchZika virusZIKV infectionGuillain-Barre syndromeMosquito salivary proteinsZika virus infectionNeurological complicationsAcute illnessFetal microcephalyPassive immunizationActive immunizationAsymptomatic infectionInflammatory responseAedes aegypti mosquitoesVirus infectionBite siteInfectionAegypti mosquitoesImmunizationMiceSalivary proteinsAgBR1MosquitoesComplicationsHeadacheVaccination
2019
Aedes aegypti NeSt1 Protein Enhances Zika Virus Pathogenesis by Activating Neutrophils
Hastings AK, Uraki R, Gaitsch H, Dhaliwal K, Stanley S, Sproch H, Williamson E, MacNeil T, Marin-Lopez A, Hwang J, Wang Y, Grover JR, Fikrig E. Aedes aegypti NeSt1 Protein Enhances Zika Virus Pathogenesis by Activating Neutrophils. Journal Of Virology 2019, 93: 10.1128/jvi.00395-19. PMID: 30971475, PMCID: PMC6580965, DOI: 10.1128/jvi.00395-19.Peer-Reviewed Original ResearchConceptsVirus infectionBite siteMosquito salivaImmune cellsZika virusLocal immune environmentMosquito bite siteZika virus pathogenesisEarly viral replicationZika virus infectionFactor 1Pathogenesis of flavivirusesVirus-infected mosquitoesVirus-induced pathogenesisCXCL2 expressionImmune environmentPassive immunizationZIKV pathogenesisImmune microenvironmentAntibody responseZIKV replicationVirus pathogenesisMosquito bitesPrimary mouse neutrophilsImmune system
2005
Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus
Gould LH, Sui J, Foellmer H, Oliphant T, Wang T, Ledizet M, Murakami A, Noonan K, Lambeth C, Kar K, Anderson JF, de Silva AM, Diamond MS, Koski RA, Marasco WA, Fikrig E. Protective and Therapeutic Capacity of Human Single-Chain Fv-Fc Fusion Proteins against West Nile Virus. Journal Of Virology 2005, 79: 14606-14613. PMID: 16282460, PMCID: PMC1287547, DOI: 10.1128/jvi.79.23.14606-14613.2005.Peer-Reviewed Original ResearchConceptsWest Nile virus infectionWest Nile virusVirus infectionNile virusLethal West Nile virus infectionFc fusion proteinEnvelope proteinShort-term prophylaxisRecombinant human scFvsHuman single-chain FvWest Nile virus envelope proteinVariable region antibodiesPassive immunizationFlavivirus infectionVirus envelope proteinHuman vaccinesDay 1Dengue virusHuman scFvRegion antibodiesInfectionTherapeutic capacityMiceSerotype 2Single-chain Fv
2001
West Nile Virus Envelope Protein
WANG T, ANDERSON J, MAGNARELLI L, BUSHMICH S, WONG S, KOSKI R, FIKRIG E. West Nile Virus Envelope Protein. Annals Of The New York Academy Of Sciences 2001, 951: 325-327. PMID: 11797789, DOI: 10.1111/j.1749-6632.2001.tb02708.x.Peer-Reviewed Original ResearchConceptsWest Nile virus envelope proteinVirus envelope proteinWest Nile virus infectionRole of antibodiesE proteinEnvelope proteinWest Nile virusPassive immunizationHumoral responseWest Nile virus E proteinVirus infectionVirus E proteinNile virusProtein serumInfectionAntibodiesRecombinant formPatientsImmunizationProteinMiceDiagnosisImmunitySerum
1995
An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies.
Fikrig E, Liu B, Fu LL, Das S, Smallwood JI, Flavell RA, Persing DH, Schoen RT, Barthold SW, Malawista SE. An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies. The Journal Of Immunology 1995, 155: 5700-4. PMID: 7499856, DOI: 10.4049/jimmunol.155.12.5700.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupFemaleFrameshift MutationHumansLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataProtein BindingSynovial FluidConceptsSurface protein AOuter surface protein ASynovial fluidChronic Lyme arthritisSynovial fluid samplesSeparate time pointsImmune effectivenessLyme arthritisPassive immunizationProtective antibodiesHuman infectionsHuman AbsProtein ATime pointsNatural infectionInfectionBorrelia burgdorferiMiceOnly factorHuman hostFluid samplesOspAInfected hostHuman materialMicrobial persistence
1993
Inability of truncated recombinant Osp A proteins to elicit protective immunity to Borrelia burgdorferi in mice.
Bockenstedt LK, Fikrig E, Barthold SW, Kantor FS, Flavell RA. Inability of truncated recombinant Osp A proteins to elicit protective immunity to Borrelia burgdorferi in mice. The Journal Of Immunology 1993, 151: 900-6. PMID: 8335917, DOI: 10.4049/jimmunol.151.2.900.Peer-Reviewed Original ResearchConceptsA antibodiesOsp AProtective immunityMurine immune responseBorrelia burgdorferiElicit protective immunityGroups of miceDevelopment of antibodiesOuter surface protein A.Vaccine AgsChallenge infectionPassive immunizationActive immunizationImmune responseEtiologic agentIndirect immunofluorescenceLyme diseaseMiceVaccinationA antiseraAntibodiesImmunityConformational epitopesA proteinImmunization