An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies.
Fikrig E, Liu B, Fu LL, Das S, Smallwood JI, Flavell RA, Persing DH, Schoen RT, Barthold SW, Malawista SE. An ospA frame shift, identified from DNA in Lyme arthritis synovial fluid, results in an outer surface protein A that does not bind protective antibodies. The Journal Of Immunology 1995, 155: 5700-4. PMID: 7499856, DOI: 10.4049/jimmunol.155.12.5700.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial VaccinesBorrelia burgdorferi GroupFemaleFrameshift MutationHumansLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataProtein BindingSynovial FluidConceptsSurface protein AOuter surface protein ASynovial fluidChronic Lyme arthritisSynovial fluid samplesSeparate time pointsImmune effectivenessLyme arthritisPassive immunizationProtective antibodiesHuman infectionsHuman AbsProtein ATime pointsNatural infectionInfectionBorrelia burgdorferiMiceOnly factorHuman hostFluid samplesOspAInfected hostHuman materialMicrobial persistenceBorrelia burgdorferi genes selectively expressed in the infected host.
Suk K, Das S, Sun W, Jwang B, Barthold SW, Flavell RA, Fikrig E. Borrelia burgdorferi genes selectively expressed in the infected host. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4269-4273. PMID: 7753795, PMCID: PMC41925, DOI: 10.1073/pnas.92.10.4269.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigen-Antibody ReactionsAntigens, BacterialBacterial VaccinesBase SequenceBlotting, NorthernBorrelia burgdorferi GroupDNA PrimersDNA, BacterialGene ExpressionGene Transfer TechniquesGenes, BacterialGenomic LibraryImmunizationMiceMice, Inbred C3HMolecular Sequence DataPolymerase Chain ReactionSequence Homology, Amino AcidConceptsInfected hostRNA PCRDifferential screeningScreening strategyExpression libraryB. burgdorferiSelective expressionNorthern blot analysisBlot analysisMiceBorrelia burgdorferi genesB. burgdorferi genesVivoGene transfer systemMicrobial genesScreening methodPathogenic microorganismsScreeningGenesAntigen