2023
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factors
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes
2019
Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment
Ren C, Yuan Q, Braun M, Zhang X, Petri B, Zhang J, Kim D, Guez-Haddad J, Xue W, Pan W, Fan R, Kubes P, Sun Z, Opatowsky Y, Polleux F, Karatekin E, Tang W, Wu D. Leukocyte Cytoskeleton Polarization Is Initiated by Plasma Membrane Curvature from Cell Attachment. Developmental Cell 2019, 49: 206-219.e7. PMID: 30930167, PMCID: PMC6482112, DOI: 10.1016/j.devcel.2019.02.023.Peer-Reviewed Original ResearchActinsAnimalsCell AdhesionCell MembraneCell MovementCell PolarityCell-Matrix JunctionsCytoskeletonEndotheliumFemaleGTPase-Activating ProteinsHEK293 CellsHumansLeukocytesMaleMiceMice, Inbred C57BLMice, KnockoutMinor Histocompatibility AntigensMyosin Light ChainsNeutrophilsPhosphatidylinositol PhosphatesPhosphorylationPhosphotransferases (Alcohol Group Acceptor)Signal Transduction
2018
Toward a unified picture of the exocytotic fusion pore
Karatekin E. Toward a unified picture of the exocytotic fusion pore. FEBS Letters 2018, 592: 3563-3585. PMID: 30317539, PMCID: PMC6353554, DOI: 10.1002/1873-3468.13270.Peer-Reviewed Original Research
2010
A fast, single-vesicle fusion assay mimics physiological SNARE requirements
Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.Peer-Reviewed Original Research
2000
Neuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles
Sulzer D, Bogulavsky J, Larsen K, Behr G, Karatekin E, Kleinman M, Turro N, Krantz D, Edwards R, Greene L, Zecca L. Neuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 11869-11874. PMID: 11050221, PMCID: PMC17261, DOI: 10.1073/pnas.97.22.11869.Peer-Reviewed Original ResearchConceptsCytosolic catecholaminesSubstantia nigraDisease neurodegenerationCytosolic dopamineRat substantia nigraSynaptic vesiclesParkinson's disease neurodegenerationHuman substantia nigraIron chelator desferrioxamineCatecholamine neuronsFunction of neuromelaninDeep brain regionsParkinson's diseaseAdenoviral-mediated overexpressionBrain regionsDouble-membrane autophagic vacuolesVesicular accumulationPC12 cell culturesChelator desferrioxamineDopamineNM granulesAntioxidant mechanismsPC12 cellsNeuromelaninNeurite outgrowth