2024
Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes
Yan Q, Gomis Perez C, Karatekin E. Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes. Physiology 2024, 39: 231-245. PMID: 38501962, PMCID: PMC11368524, DOI: 10.1152/physiol.00007.2024.Peer-Reviewed Original ResearchDetection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution
Landajuela A, Braun M, Rodrigues C, Karatekin E. Detection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution. STAR Protocols 2024, 5: 102965. PMID: 38502684, PMCID: PMC10963649, DOI: 10.1016/j.xpro.2024.102965.Peer-Reviewed Original Research
2021
A human apolipoprotein L with detergent-like activity kills intracellular pathogens
Gaudet RG, Zhu S, Halder A, Kim BH, Bradfield CJ, Huang S, Xu D, Mamiñska A, Nguyen TN, Lazarou M, Karatekin E, Gupta K, MacMicking JD. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 2021, 373 PMID: 34437126, PMCID: PMC8422858, DOI: 10.1126/science.abf8113.Peer-Reviewed Original ResearchMeSH KeywordsApolipoproteins LBacterial Outer MembraneBacteriolysisCell MembraneCell Membrane PermeabilityCells, CulturedCRISPR-Cas SystemsCytosolDetergentsGene EditingGram-Negative BacteriaGTP-Binding ProteinsHumansImmunity, InnateInterferon-gammaLipoproteinsMicrobial ViabilityO AntigensProtein DomainsSalmonella typhimuriumSolubilityConceptsSingle-particle cryo-electron microscopyCell-autonomous defenseCytosol-invasive bacteriaExpression of hundredsNative mass spectrometryCryo-electron microscopyHuman genesDetergent-like activityHost proteinsLipoprotein nanodiscsMammalian lipidsExtracellular transportImmune cytokine interferonCell typesDetergent-like propertiesApolipoprotein LLife-threatening infectionsPotent bactericidal agentsAnionic membranesProteinCytokine interferonNonimmune cellsMass spectrometryCellsMutagenesis
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes
2016
Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains
Wu Z, Auclair SM, Bello O, Vennekate W, Dudzinski NR, Krishnakumar SS, Karatekin E. Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Scientific Reports 2016, 6: 27287. PMID: 27264104, PMCID: PMC4893671, DOI: 10.1038/srep27287.Peer-Reviewed Original ResearchConceptsFusion poreTransmembrane domainPore dynamicsProtein transmembrane domainNeurotransmitter-filled vesiclesT-SNAREsPlasma membraneRecycling kineticsPore lifetimePore currentsFlickering poresPore stabilityMultiple timesZipperingNanodiscsDomainProteinVesiclesMembraneCellsAssaysCognatesPore propertiesPoresFusion Between v-SNARE Nanodiscs and “Flipped” t-SNARE Cells: Control of Fusion Pore Nucleation and Lifetimes by SNARE Protein Transmembrane Domains
Wu Z, Auclair S, Bello O, Vennekate W, Dudzinski N, Krishnakumar S, Karatekin E. Fusion Between v-SNARE Nanodiscs and “Flipped” t-SNARE Cells: Control of Fusion Pore Nucleation and Lifetimes by SNARE Protein Transmembrane Domains. Biophysical Journal 2016, 110: 430a. DOI: 10.1016/j.bpj.2015.11.2321.Peer-Reviewed Original Research
2011
Back Cover: Coupling Amperometry and Total Internal Reflection Fluorescence Microscopy at ITO Surfaces for Monitoring Exocytosis of Single Vesicles (Angew. Chem. Int. Ed. 22/2011)
Meunier A, Jouannot O, Fulcrand R, Fanget I, Bretou M, Karatekin E, Arbault S, Guille M, Darchen F, Lemaître F, Amatore C. Back Cover: Coupling Amperometry and Total Internal Reflection Fluorescence Microscopy at ITO Surfaces for Monitoring Exocytosis of Single Vesicles (Angew. Chem. Int. Ed. 22/2011). Angewandte Chemie International Edition 2011, 50 DOI: 10.1002/anie.201102239.Peer-Reviewed Original ResearchTotal internal reflection fluorescence microscopyReflection fluorescence microscopyFluorescence microscopySingle living cellsCellular organismsSingle exocytotic eventsMolecular messengersExocytotic eventsLiving cellsSingle vesiclesExtracellular mediumExocytosisKey mechanismOrganismsMessengerVesiclesCellsMicroscopy
2010
A fast, single-vesicle fusion assay mimics physiological SNARE requirements
Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 3517-3521. PMID: 20133592, PMCID: PMC2840481, DOI: 10.1073/pnas.0914723107.Peer-Reviewed Original Research