2015
Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments
Durer Z, McGillivary RM, Kang H, Elam WA, Vizcarra CL, Hanein D, De La Cruz EM, Reisler E, Quinlan ME. Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments. Journal Of Molecular Biology 2015, 427: 2782-2798. PMID: 26168869, PMCID: PMC4540644, DOI: 10.1016/j.jmb.2015.07.005.Peer-Reviewed Original ResearchConceptsMetavinculin tail domainVinculin tail domainActin filamentsTail domainSevering activityCell-extracellular matrix junctionsF-actinC-terminal tail domainTotal internal reflection fluorescence microscopy experimentsLonger splice isoformsLimited proteolysis experimentsActin filament bundlesFluorescence microscopy experimentsMatrix junctionsSite-directed labelingSplice isoformsAbundant proteinsProteolysis experimentsMuscle cell functionFilament organizationVinculinFilament bundlesInterprotomer contactsCell functionFilament flexibility
2011
Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain
Prochniewicz E, Pierre A, McCullough BR, Chin HF, Cao W, Saunders LP, Thomas DD, De La Cruz EM. Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain. Journal Of Molecular Biology 2011, 413: 584-592. PMID: 21910998, PMCID: PMC3633491, DOI: 10.1016/j.jmb.2011.08.058.Peer-Reviewed Original ResearchConceptsActin filament dynamicsMyosin VIFilament dynamicsMicrosecond dynamicsCaM-dependent mannerCalmodulin light chainsLight chainActin bindingActin filamentsDependent CaMIQ domainCaM-dependent regulationFluorescence microscopyEnzymatic activityTransient phosphorescence anisotropyATP utilizationFinal anisotropyMicrosecond rotational dynamicsPhosphorescence anisotropyMyosinStructural dynamicsAnisotropy decaySuch modulationActinRegulation
2006
Energetics and Kinetics of Cooperative Cofilin–Actin Filament Interactions
Cao W, Goodarzi JP, De La Cruz EM. Energetics and Kinetics of Cooperative Cofilin–Actin Filament Interactions. Journal Of Molecular Biology 2006, 361: 257-267. PMID: 16843490, DOI: 10.1016/j.jmb.2006.06.019.Peer-Reviewed Original Research
2004
Cofilin Binding to Muscle and Non-muscle Actin Filaments: Isoform-dependent Cooperative Interactions
De La Cruz EM. Cofilin Binding to Muscle and Non-muscle Actin Filaments: Isoform-dependent Cooperative Interactions. Journal Of Molecular Biology 2004, 346: 557-564. PMID: 15670604, DOI: 10.1016/j.jmb.2004.11.065.Peer-Reviewed Original ResearchConceptsActin filamentsConcentration of cofilinActin isoformsFilament severingRegulatory proteinsCooperative free energyActin fluorescencePyrene-actin fluorescenceCofilinFilament bindingHuman cofilinEquilibrium bindingCooperative interactionsIntrinsic affinitySkeletal muscleIsoformsBindingFilamentsSeveringMuscle actinHill coefficientCooperativity modelFilament latticeIsolated siteProteinMechanochemical coupling of two substeps in a single myosin V motor
Uemura S, Higuchi H, Olivares AO, De La Cruz EM, Ishiwata S. Mechanochemical coupling of two substeps in a single myosin V motor. Nature Structural & Molecular Biology 2004, 11: 877-883. PMID: 15286720, DOI: 10.1038/nsmb806.Peer-Reviewed Original ResearchMechanism of Nucleotide Binding to Actomyosin VI EVIDENCE FOR ALLOSTERIC HEAD-HEAD COMMUNICATION*
Robblee JP, Olivares AO, De La Cruz EM. Mechanism of Nucleotide Binding to Actomyosin VI EVIDENCE FOR ALLOSTERIC HEAD-HEAD COMMUNICATION*. Journal Of Biological Chemistry 2004, 279: 38608-38617. PMID: 15247304, DOI: 10.1074/jbc.m403504200.Peer-Reviewed Original ResearchActinsActomyosinAdenosine DiphosphateAdenosine TriphosphatasesAdenosine TriphosphateAllosteric SiteAnimalsCell LineDose-Response Relationship, DrugHydrogen-Ion ConcentrationInsectaKineticsModels, BiologicalModels, ChemicalModels, StatisticalMuscle, SkeletalMyosin Heavy ChainsNucleotidesProtein BindingPyrenesRabbitsTime Factors
2000
Actin and Light Chain Isoform Dependence of Myosin V Kinetics †
De La Cruz E, Wells A, Sweeney H, Ostap E. Actin and Light Chain Isoform Dependence of Myosin V Kinetics †. Biochemistry 2000, 39: 14196-14202. PMID: 11087368, DOI: 10.1021/bi001701b.Peer-Reviewed Original ResearchThymosin-β4 Changes the Conformation and Dynamics of Actin Monomers
De La Cruz E, Ostap E, Brundage R, Reddy K, Sweeney H, Safer D. Thymosin-β4 Changes the Conformation and Dynamics of Actin Monomers. Biophysical Journal 2000, 78: 2516-2527. PMID: 10777749, PMCID: PMC1300842, DOI: 10.1016/s0006-3495(00)76797-x.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBinding SitesBiophysical PhenomenaBiophysicsCircular DichroismCross-Linking ReagentsFluorescent DyesHumansIn Vitro TechniquesKineticsMacromolecular SubstancesModels, MolecularMuscle, SkeletalMutagenesis, Site-DirectedNaphthalenesulfonatesOsmotic PressureProtein BindingProtein ConformationRabbitsRecombinant ProteinsThermodynamicsThymosinTritiumViscosityConceptsActin monomersActin monomer poolMgATP-actinMetazoan cellsNucleotide exchangeC-terminusSubdomain 2Conformational changesMonomer poolN-iodoacetyl-N'Cleavage siteActinThymosin β4Rate of dissociationAmide protonsSulfo-1Unique abilityBindingStructural dynamicsConformationTerminusNucleotidesProteolysisDifferent stabilitiesDegrees C
1996
Kinetics and Thermodynamics of Phalloidin Binding to Actin Filaments from Three Divergent Species †
De La Cruz E, Pollard T. Kinetics and Thermodynamics of Phalloidin Binding to Actin Filaments from Three Divergent Species †. Biochemistry 1996, 35: 14054-14061. PMID: 8916890, DOI: 10.1021/bi961047t.Peer-Reviewed Original Research
1995
Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics.
De La Cruz EM, Pollard TD. Nucleotide-free actin: stabilization by sucrose and nucleotide binding kinetics. Biochemistry 1995, 34: 5452-61. PMID: 7727403, DOI: 10.1021/bi00016a016.Peer-Reviewed Original Research