2015
Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments
Durer Z, McGillivary RM, Kang H, Elam WA, Vizcarra CL, Hanein D, De La Cruz EM, Reisler E, Quinlan ME. Metavinculin Tunes the Flexibility and the Architecture of Vinculin-Induced Bundles of Actin Filaments. Journal Of Molecular Biology 2015, 427: 2782-2798. PMID: 26168869, PMCID: PMC4540644, DOI: 10.1016/j.jmb.2015.07.005.Peer-Reviewed Original ResearchConceptsMetavinculin tail domainVinculin tail domainActin filamentsTail domainSevering activityCell-extracellular matrix junctionsF-actinC-terminal tail domainTotal internal reflection fluorescence microscopy experimentsLonger splice isoformsLimited proteolysis experimentsActin filament bundlesFluorescence microscopy experimentsMatrix junctionsSite-directed labelingSplice isoformsAbundant proteinsProteolysis experimentsMuscle cell functionFilament organizationVinculinFilament bundlesInterprotomer contactsCell functionFilament flexibility
2005
Vertebrate Myosin VIIb Is a High Duty Ratio Motor Adapted for Generating and Maintaining Tension*
Henn A, De La Cruz EM. Vertebrate Myosin VIIb Is a High Duty Ratio Motor Adapted for Generating and Maintaining Tension*. Journal Of Biological Chemistry 2005, 280: 39665-39676. PMID: 16186105, DOI: 10.1074/jbc.m507667200.Peer-Reviewed Original ResearchConceptsClass VII myosinMyosin VIIbActin filament concentrationUnique cellular functionsInner ear hair cellsCatalytic motor domainEar hair cellsActin filament bundlesNon-muscle myosinSteady-state ATPase activityActin cytoskeletonHigh conservationCellular functionsMyosin VIIa functionCDNA libraryActin bindingDeafness phenotypeMembrane surface receptorsATPase cycleEnzymatic propertiesSurface receptorsFilament bundlesIntestinal brush borderMolecular motorsQuantitative equilibrium