2024
Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides
Reshetnyak Y, Andreev O, Engelman D. Aiming the magic bullet: targeted delivery of imaging and therapeutic agents to solid tumors by pHLIP peptides. Frontiers In Pharmacology 2024, 15: 1355893. PMID: 38545547, PMCID: PMC10965573, DOI: 10.3389/fphar.2024.1355893.Peer-Reviewed Original ResearchTumor microenvironmentTherapeutic agents to solid tumorsTargeted intracellular deliverySolid tumorsImmune cellsPHLIP technologyTargeted delivery of imagingPre-clinicalDelivery of imagingTherapeutic payloadsTumorTargeted deliveryIntracellular deliveryTumor imagingSimultaneous targetingClinical applicationTherapeutic deliveryCell typesSurface of cellsPHLIP peptidePHLIPDeliveryTarget biomarkersCellsMagic bulletTargeted intracellular delivery of dimeric STINGa by two pHLIP peptides for treatment of solid tumors
Moshnikova A, DuPont M, Iraca M, Klumpp C, Visca H, Allababidi D, Pelzer P, Engelman D, Andreev O, Reshetnyak Y. Targeted intracellular delivery of dimeric STINGa by two pHLIP peptides for treatment of solid tumors. Frontiers In Pharmacology 2024, 15: 1346756. PMID: 38495104, PMCID: PMC10940318, DOI: 10.3389/fphar.2024.1346756.Peer-Reviewed Original ResearchTargeted intracellular deliveryIntracellular deliveryTreatment of solid tumorsAnti-cancer immunityPHLIP peptideBinding to plasma proteinsBlood half-lifeTumor eradicationSolid tumorsCytokine activityTumorTargeted deliveryDelivery approachesHalf-lifePlasma proteinsDMSAPHLIPDeliveryMembrane lipid bilayerPeptidepHLIP targeted intracellular delivery of calicheamicin
DuPont M, Klumpp C, Iraca M, Allababidi D, Visca H, Engelman D, Andreev O, Moshnikova A, Reshetnyak Y. pHLIP targeted intracellular delivery of calicheamicin. International Journal Of Pharmaceutics 2024, 654: 123954. PMID: 38428548, DOI: 10.1016/j.ijpharm.2024.123954.Peer-Reviewed Original ResearchM2 tumor-associated macrophagesTreatment of solid tumorsTumor-associated macrophagesTherapeutic agents to tumorsTumor-stroma interfaceAgents to tumorsInhibited tumor growthPolarized M2 macrophagesTreatment of tumorsAntibody-drug conjugatesTumor massLiquid tumorsSolid tumorsImmune cellsTumor growthM2 macrophagesTumorPH low insertion peptideBiodistribution studiesIntracellular deliveryIn vivoTumor coreCalicheamicinMacrophagesTreatment
2023
Tumor treatment by pHLIP-targeted antigen delivery
DuPont M, Visca H, Moshnikova A, Engelman D, Reshetnyak Y, Andreev O. Tumor treatment by pHLIP-targeted antigen delivery. Frontiers In Bioengineering And Biotechnology 2023, 10: 1082290. PMID: 36686229, PMCID: PMC9853002, DOI: 10.3389/fbioe.2022.1082290.Peer-Reviewed Original ResearchAnti-HA antibodiesAntigen deliveryHigh titersTumor cellsTriple-negative breast tumorsCancer cellsDifferent vaccination schemesNegative breast tumorsHA epitopeAntigen therapyImmunized miceImmune cellsNeo-antigensT cellsSignificant tumorsVaccination schemeViral infectionTherapeutic efficacyBreast tumorsHepatic clearanceImmune systemMelanoma tumorsTumorsPeptide epitopesAdjuvant
2022
Eradication of tumors and development of anti-cancer immunity using STINGa targeted by pHLIP
Moshnikova A, DuPont M, Visca H, Engelman D, Andreev O, Reshetnyak Y. Eradication of tumors and development of anti-cancer immunity using STINGa targeted by pHLIP. Frontiers In Oncology 2022, 12: 1023959. PMID: 36330464, PMCID: PMC9622777, DOI: 10.3389/fonc.2022.1023959.Peer-Reviewed Original ResearchTumor-associated macrophagesCancer-associated fibroblastsSTING agonistsT cellsTumor microenvironmentDendritic cellsCT26 tumorsM2-type tumor-associated macrophagesAnti-cancer immunityTumor-free miceEradication of tumorsActivation of cytokinesNovel STING agonistSuppressor cellsSingle doseImmune cellsIntratumoral hemorrhageTherapeutic effectTumor stromaImmune memoryNude miceAdditional injectionTumorsCancer cellsMiceTargeting bladder urothelial carcinoma with pHLIP-ICG and inhibition of urothelial cancer cell proliferation by pHLIP-amanitin
Moshnikova A, Golijanin B, Amin A, Doyle J, Kott O, Gershman B, DuPont M, Li Y, Lu X, Engelman D, Andreev O, Reshetnyak Y, Golijanin D. Targeting bladder urothelial carcinoma with pHLIP-ICG and inhibition of urothelial cancer cell proliferation by pHLIP-amanitin. Frontiers In Urology 2022, 2: 868919. PMID: 36439552, PMCID: PMC9691284, DOI: 10.3389/fruro.2022.868919.Peer-Reviewed Original ResearchWhite light cystoscopyCancer cell proliferationUrothelial cancer cellsMalignant lesionsUrothelial cancerBladder cancerCancer cellsSuperficial bladder cancerLaparoscopic radical cystectomyBladder urothelial carcinomaCell proliferationHr of treatmentRadical cystectomyIntravesical instillationBladder specimensUrothelial carcinomaUseful biomarkerNIRF imagingHuman bladderSitu casesDifferent subtypesHistopathological processingCystoscopyTumor cellsCancer
2021
Cell Boundaries, How Membranes and Their Proteins Work
White S, von Heijne G, Engelman D. Cell Boundaries, How Membranes and Their Proteins Work. 2021 DOI: 10.1201/9780429341328.Peer-Reviewed Original ResearchHigh-resolution micrographsMembrane proteinsMolecular mechanismsOrganization of lipidsBasic physicsWide arrayCell membranePhysicsProteinCentral discoveryBiologyPhysics studentsMembraneBiophysical foundationConcerted useCell boundariesDiscoveryPhysical chemistryAdvanced undergraduateDiagramArrayFoldingOrganizational principlesStructureMicrographs
2019
On the Folding and Insertion of Globular Membrane Proteins
Wetlaufer D, Engelman D, Steitz T. On the Folding and Insertion of Globular Membrane Proteins. 2019, 87-113. DOI: 10.4324/9780429314148-5.Peer-Reviewed Original Research
2018
Acidity at the Surfaces of Cancer Cells
Andreev O, Wei D, Engelman D, Reshetnyak Y. Acidity at the Surfaces of Cancer Cells. Biophysical Journal 2018, 114: 359a. DOI: 10.1016/j.bpj.2017.11.1996.Peer-Reviewed Original Research
2017
69 Novel ex vivo endoscopic near infrared fluorescence imaging method using pHLIP®/ICG in patients undergoing radical cystectomy for urothelial carcinoma of the bladder
Brito J, Golijanin B, Tran T, Moshnikova A, Gershman B, Engelman D, Reshetnyak Y, Andreev O, Amin A, Golijanin D. 69 Novel ex vivo endoscopic near infrared fluorescence imaging method using pHLIP®/ICG in patients undergoing radical cystectomy for urothelial carcinoma of the bladder. European Urology Open Science 2017, 16: e116-e117. DOI: 10.1016/s1569-9056(17)30136-7.Peer-Reviewed Original Research
2016
pHLIP®: Uses in Measuring Cell Surface pH, Imaging Tumors, and Delivering Therapeutics
Engelman D, Reshetnyak Y, Andreev O. pHLIP®: Uses in Measuring Cell Surface pH, Imaging Tumors, and Delivering Therapeutics. Biophysical Journal 2016, 110: 418a. DOI: 10.1016/j.bpj.2015.11.2259.Peer-Reviewed Original Research
2015
Lipid Composition Influences the Insertion and Folding of pHLIP Peptides
Karabadzhak A, Weerakkody D, Andreev O, Reshetnyak Y, Engelman D. Lipid Composition Influences the Insertion and Folding of pHLIP Peptides. Biophysical Journal 2015, 108: 555a. DOI: 10.1016/j.bpj.2014.11.3042.Peer-Reviewed Original ResearchpHLIP® Targeting and Delivery of PNA to Silence MicroRNA in Tumor Cells§
Engelman D, Cheng C, Bahal R, Babar I, Pincus Z, Barrera F, Liu C, Svoronos A, Braddock D, Glazer P, Saltzman W, Slack F. pHLIP® Targeting and Delivery of PNA to Silence MicroRNA in Tumor Cells§. Biophysical Journal 2015, 108: 552a. DOI: 10.1016/j.bpj.2014.11.3029.Peer-Reviewed Original Research
2014
Imaging and Treating Tumors by Targeting their Acidity with Phlip, a Ph-Sensitve Insertion Peptide
Engelman D, An M, Andreev O, Barrera F, Bahal R, Bosenberg M, Cheng C, Glazer P, Karabadzhak A, Reshetnyak Y, Saltzman W, Slack F, Svoronos A, Thevenin D. Imaging and Treating Tumors by Targeting their Acidity with Phlip, a Ph-Sensitve Insertion Peptide. Biophysical Journal 2014, 106: 231a. DOI: 10.1016/j.bpj.2013.11.1350.Peer-Reviewed Original ResearchPhlip-Fire: A High-Contrast, Insertion-Triggered Fluorescent Probe for Targeting Tumors In Vivo
Karabadzhak A, Yao L, Langenbacher R, Moshnikova A, Adochite R, An M, Andreev O, Reshetnyak Y, Engelman D. Phlip-Fire: A High-Contrast, Insertion-Triggered Fluorescent Probe for Targeting Tumors In Vivo. Biophysical Journal 2014, 106: 88a-89a. DOI: 10.1016/j.bpj.2013.11.561.Peer-Reviewed Original Research
2013
Targeting of Melanoma by pH (Low) Insertion Peptide (pHLIP)
Svoronos A, Cheng C, Barrera F, Saltzman W, Bosenberg M, Engelman D. Targeting of Melanoma by pH (Low) Insertion Peptide (pHLIP). Biophysical Journal 2013, 104: 677a. DOI: 10.1016/j.bpj.2012.11.3740.Peer-Reviewed Original ResearchCharacterization of an Intermediate for the Formation of the Transmembrane Helix of pHLIP Peptide
Barrera F, Karabadzhak A, Engelman D. Characterization of an Intermediate for the Formation of the Transmembrane Helix of pHLIP Peptide. Biophysical Journal 2013, 104: 596a. DOI: 10.1016/j.bpj.2012.11.3309.Peer-Reviewed Original ResearchModulation of the pHLIP Transmembrane Helix Insertion Pathway
Reshetnyak Y, Karabadzhak A, Weerakkody D, Wijesinghe D, Thakur M, Engelman D, Markin V, Andreev O. Modulation of the pHLIP Transmembrane Helix Insertion Pathway. Biophysical Journal 2013, 104: 62a-63a. DOI: 10.1016/j.bpj.2012.11.383.Peer-Reviewed Original ResearchMembrane Physical Properties Influence Transmembrane Helix Formation
Barrera F, Fendos J, Engelman D. Membrane Physical Properties Influence Transmembrane Helix Formation. Biophysical Journal 2013, 104: 535a-536a. DOI: 10.1016/j.bpj.2012.11.2965.Peer-Reviewed Original ResearchVisualizing pHLIP Insertion in Plasmamembrane and Endosomal Membrane
Yao L, Langenbacher R, Karabadzhak A, Engelman D, An M. Visualizing pHLIP Insertion in Plasmamembrane and Endosomal Membrane. Biophysical Journal 2013, 104: 238a. DOI: 10.1016/j.bpj.2012.11.1342.Peer-Reviewed Original Research