1985
Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchTRNA genesGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsSchizosaccharomyces pombeClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesMutationsSup3SchizosaccharomycesMutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchSup3-e geneTRNA genesInternal transcriptional control regionSuppression of nonsense codonsGenomic clone bankEucaryotic tRNA genesSaccharomyces cerevisiae extractsTranscriptional control regionIdentification of mutationsSchizosaccharomyces pombeColony hybridizationRevertant allelesTRNA precursorsClone bankNonsense codonSuppressor locusGenetic evidenceSequence analysisControl regionMutational hotspotsTranscription efficiencyPoint mutationsGenesMutationsSup3
1984
The Schizosaccharomyces pombe sup3‐i suppressor recognizes ochre, but not amber codons in vitro and in vivo.
Hottinger H, Stadelmann B, Pearson D, Frendewey D, Kohli J, Söll D. The Schizosaccharomyces pombe sup3‐i suppressor recognizes ochre, but not amber codons in vitro and in vivo. The EMBO Journal 1984, 3: 423-428. PMID: 6370683, PMCID: PMC557361, DOI: 10.1002/j.1460-2075.1984.tb01823.x.Peer-Reviewed Original ResearchConceptsFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeUGA termination codonVitro translation assaysReadthrough productS. pombeSchizosaccharomyces pombeNonsense mutantsTermination signalOchre suppressorUGA suppressionTranslation assaysAmber codonTermination codonGlobin mRNASup3PombeT substitutionCodonSuppressorPlasmid DNASchizosaccharomycesMutantsVivoAnticodon