1993
Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins.
Wu D, Jiang H, Katz A, Simon M. Identification of critical regions on phospholipase C-beta 1 required for activation by G-proteins. Journal Of Biological Chemistry 1993, 268: 3704-3709. PMID: 8381437, DOI: 10.1016/s0021-9258(18)53750-9.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarbacholCell LineCloning, MolecularEnzyme ActivationGTP-Binding ProteinsInositol PhosphatesKineticsMolecular Sequence DataMutagenesisNorepinephrinePhosphatidylinositol Diacylglycerol-LyasePhosphoric Diester HydrolasesPlasmidsReceptors, MuscarinicRecombinant ProteinsRestriction MappingSequence DeletionTransfectionConceptsG alpha q.PLC-beta 1COS-7 cellsG alpha qAlpha q.Alpha subunitG protein alpha subunitsAlpha qMuscarinic acetylcholine receptor type 1G alpha subunitsPhosphoinositide-specific phospholipase CIntrinsic enzymatic activitySpecific deletionAmino acid sequenceLigand-induced releasePhospholipase C beta 1C beta 1CDNA clonesAcid sequenceC-terminusG proteinsTerminal endDependent activationPhospholipase CAlpha 1C-adrenergic receptor
1992
Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunits