2016
Proximity ligation-induced assembly of DNAzymes for simple and cost-effective colourimetric detection of proteins with high sensitivity
Wei L, Wang X, Wu D, Li C, Yin Y, Li G. Proximity ligation-induced assembly of DNAzymes for simple and cost-effective colourimetric detection of proteins with high sensitivity. Chemical Communications 2016, 52: 5633-5636. PMID: 27032382, DOI: 10.1039/c6cc00205f.Peer-Reviewed Original Research
2001
Low-Density Lipoprotein Receptor-Related Protein-5 Binds to Axin and Regulates the Canonical Wnt Signaling Pathway
Mao J, Wang J, Liu B, Pan W, Farr G, Flynn C, Yuan H, Takada S, Kimelman D, Li L, Wu D. Low-Density Lipoprotein Receptor-Related Protein-5 Binds to Axin and Regulates the Canonical Wnt Signaling Pathway. Molecular Cell 2001, 7: 801-809. PMID: 11336703, DOI: 10.1016/s1097-2765(01)00224-6.Peer-Reviewed Original Research3T3 CellsAmino Acid SequenceAnimalsAxin ProteinCalcium-Calmodulin-Dependent Protein KinasesCOS CellsGene ExpressionGlycogen Synthase Kinase 3LDL-Receptor Related ProteinsLow Density Lipoprotein Receptor-Related Protein-5MammalsMiceMolecular Sequence DataMutagenesisProtein BindingProtein Structure, TertiaryProteinsProto-Oncogene ProteinsReceptors, LDLRepressor ProteinsSignal TransductionWnt ProteinsZebrafish Proteins
2000
Protein Phosphatase 2Cα Dephosphorylates Axin and Activates LEF-1-dependent Transcription*
Wu D, Strovel E, Sussman D. Protein Phosphatase 2Cα Dephosphorylates Axin and Activates LEF-1-dependent Transcription*. Journal Of Biological Chemistry 2000, 275: 2399-2403. PMID: 10644691, DOI: 10.1074/jbc.275.4.2399.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAxin ProteinCOS CellsDishevelled ProteinsDNA-Binding ProteinsEnzyme ActivationJNK Mitogen-Activated Protein KinasesLymphoid Enhancer-Binding Factor 1Mitogen-Activated Protein KinasesPhosphoprotein PhosphatasesPhosphoproteinsPhosphorylationPrecipitin TestsProtein BindingProtein Phosphatase 2Protein Phosphatase 2CProteinsRepressor ProteinsSaccharomyces cerevisiae ProteinsSignal TransductionTranscription FactorsTranscriptional ActivationConceptsLEF-1-dependent transcriptionWnt signal transductionSignal transductionExpression of PP2CProtein phosphatase 2CalphaProtein phosphatase 2CαTwo-hybrid systemDishevelled gene familyReporter gene assayGene familyPositive regulatorCytoplasmic proteinsPP2CNegative regulatorAxinWnt-1Gene assayTranscriptionTransductionRegulatorSynergistic responseProteinExpressionDephosphorylationRepression
1999
Suppression of Glycogen Synthase Kinase Activity Is Not Sufficient for Leukemia Enhancer Factor-1 Activation*
Yuan H, Mao J, Li L, Wu D. Suppression of Glycogen Synthase Kinase Activity Is Not Sufficient for Leukemia Enhancer Factor-1 Activation*. Journal Of Biological Chemistry 1999, 274: 30419-30423. PMID: 10521419, DOI: 10.1074/jbc.274.43.30419.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAdaptor Proteins, Signal TransducingAnimalsAxin ProteinBeta CateninCalcium-Calmodulin-Dependent Protein KinasesCarrier ProteinsCytoskeletal ProteinsDNA-Binding ProteinsEnzyme ActivationGenes, ReporterGlycogen Synthase Kinase 3Glycogen Synthase KinasesLuciferasesLymphoid Enhancer-Binding Factor 1MiceNeoplasm ProteinsPhosphorylationProtein Serine-Threonine KinasesProteinsProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRecombinant ProteinsRepressor ProteinsSignal TransductionTrans-ActivatorsTranscription FactorsTranscription, GeneticTransfectionWnt ProteinsWnt1 ProteinZebrafish ProteinsConceptsGlycogen synthase kinase-3LEF-1-dependent transcriptionKinase activityWnt proteinsWnt-1Glycogen synthase kinase activitySynthase kinase activityProtein kinase AktSynthase kinase-3Enhancer factor-1Kinase AktAxinKinase 3MAktPeptide substratesReduced associationAktPhe residueTyr-216Factor 1TranscriptionFRATProteinActivationAdditional mechanismAxin and Frat1 interact with Dvl and GSK, bridging Dvl to GSK in Wnt‐mediated regulation of LEF‐1
Li L, Yuan H, Weaver C, Mao J, Farr G, Sussman D, Jonkers J, Kimelman D, Wu D. Axin and Frat1 interact with Dvl and GSK, bridging Dvl to GSK in Wnt‐mediated regulation of LEF‐1. The EMBO Journal 1999, 18: 4233-4240. PMID: 10428961, PMCID: PMC1171499, DOI: 10.1093/emboj/18.15.4233.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAxin ProteinCalcium-Calmodulin-Dependent Protein KinasesDishevelled ProteinsDNA-Binding ProteinsGlycogen Synthase Kinase 3Glycogen Synthase KinasesLymphoid Enhancer-Binding Factor 1PhosphoproteinsProtein BindingProtein ConformationProteinsProto-Oncogene ProteinsRepressor ProteinsSignal TransductionTranscription FactorsWnt ProteinsWnt1 ProteinXenopusXenopus ProteinsZebrafish Proteins
1998
Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13
Mao J, Yuan H, Xie W, Wu D. Guanine nucleotide exchange factor GEF115 specifically mediates activation of Rho and serum response factor by the G protein α subunit Gα13. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12973-12976. PMID: 9789025, PMCID: PMC23675, DOI: 10.1073/pnas.95.22.12973.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SequenceAnimalsCOS CellsDNA-Binding ProteinsGene Expression RegulationGTP-Binding ProteinsGTPase-Activating ProteinsGuanine Nucleotide Exchange FactorsKineticsLuciferasesMiceMolecular Sequence DataNuclear ProteinsProteinsRecombinant Fusion ProteinsRhoA GTP-Binding ProteinSequence AlignmentSequence Homology, Amino AcidSerum Response FactorSignal TransductionTranscription FactorsTranscription, GeneticTransfectionConceptsSerum response factorActivation of RhoSRF activationHeterotrimeric G protein alpha subunitsG protein signaling (RGS) domainG protein alpha subunitsTerminal partRho-specific guanineProtein alpha subunitsResponse factorDomain deletion mutantSignal transduction pathwaysNIH 3T3 cellsDbl homologyRGS activitySequence motifsTransduction pathwaysGalpha13Signaling domainsGalpha12Alpha subunitMutantsSynergistic activationGalphaqTransfection systemSpecific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*
Mao J, Yuan H, Xie W, Simon M, Wu D. Specific Involvement of G Proteins in Regulation of Serum Response Factor-mediated Gene Transcription by Different Receptors*. Journal Of Biological Chemistry 1998, 273: 27118-27123. PMID: 9765229, DOI: 10.1074/jbc.273.42.27118.Peer-Reviewed Original ResearchMeSH Keywords15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid3T3 CellsAnimalsDNA-Binding ProteinsDrug SynergismEndothelinsGene Expression RegulationGTP-Binding ProteinsGTPase-Activating ProteinsLysophospholipidsMiceNuclear ProteinsProteinsReceptors, Cell SurfaceSerum Response FactorThrombinTranscription, GeneticConceptsThromboxane A2Lysophosphatidic acidType 1 muscarinic receptorG proteinsAlpha1-adrenergic receptorsInterleukin-8 receptorEndogenous G proteinsG protein-coupled receptorsProtein-coupled receptorsEndothelin receptorsMuscarinic receptorsDopamine receptorsSRF activationSerum response factorType 1Gene transcriptionReceptorsDifferent receptorsG protein subunitsSpecific involvementGalphaq/11Gq classCell linesNIH3T3 cellsThrombin