2001
Gα16 Couples Chemoattractant Receptors to NF-κB Activation
Yang M, Sang H, Rahman A, Wu D, Malik A, Ye R. Gα16 Couples Chemoattractant Receptors to NF-κB Activation. The Journal Of Immunology 2001, 166: 6885-6892. PMID: 11359849, DOI: 10.4049/jimmunol.166.11.6885.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAmino Acid SubstitutionCell NucleusEnzyme ActivationGenes, ReporterGlutamineGTP-Binding Protein alpha Subunits, Gq-G11HeLa CellsHeterotrimeric GTP-Binding ProteinsHumansIsoenzymesLeucineLuciferasesNF-kappa BPhospholipase C betaReceptors, ChemokineReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTrans-ActivatorsTransfectionType C PhospholipasesConceptsNF-kappaB activationFormyl peptide receptorChemoattractant receptorsPeptide receptorCXC chemokine receptor 2NF-kappaB.Chemokine receptor 2Regulatory protein alpha subunitNF-κB activationLuciferase activityProtein kinase CKappaB-luciferase activityPhosphorylation of IkappaBalphaPhospholipase C-beta activityC3a receptorReceptor 2Seven-membrane-spanning receptorsAgonist stimulationConventional PKC isoformsPharmacological inhibitorsLuciferase reporterReceptorsHemopoietic cellsProtein alpha subunitsCell model systemDeficient long‐term synaptic depression in the rostral cerebellum correlated with impaired motor learning in phospholipase C β4 mutant mice
Miyata M, Kim H, Hashimoto K, Lee T, Cho S, Jiang H, Wu Y, Jun K, Wu D, Kano M, Shin H. Deficient long‐term synaptic depression in the rostral cerebellum correlated with impaired motor learning in phospholipase C β4 mutant mice. European Journal Of Neuroscience 2001, 13: 1945-1954. PMID: 11403688, DOI: 10.1046/j.0953-816x.2001.01570.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxiaBlinkingCalcium SignalingCerebellumConditioning, PsychologicalIsoenzymesLearningLong-Term PotentiationMiceMice, KnockoutMotor ActivityMutationNerve FibersNeural InhibitionPhospholipase C betaPurkinje CellsReceptors, Metabotropic GlutamateReference ValuesSynapsesSynaptic TransmissionType C PhospholipasesConceptsLong-term depressionRostral cerebellumMutant micePurkinje cellsMetabotropic glutamate receptor subtype 1Parallel fiber-Purkinje cell synapseParallel fiber-Purkinje cell synapsesLong-term synaptic depressionFiber-Purkinje cell synapseFiber-Purkinje cell synapsesMotor learningReceptor subtype 1Voltage-gated Ca2Wild-type miceParallel fiber stimulationEyeblink responseMost Purkinje cellsUnconditioned stimulusImpaired motorCell synapsesCaudal cerebellumSynaptic depressionCell synapseFiber stimulationSubtype 1Calcium Responses to Thyrotropin-Releasing Hormone, Gonadotropin-Releasing Hormone and Somatostatin in Phospholipase Cβ3 Knockout Mice
Romoser V, Graves T, Wu D, Jiang H, Hinkle P. Calcium Responses to Thyrotropin-Releasing Hormone, Gonadotropin-Releasing Hormone and Somatostatin in Phospholipase Cβ3 Knockout Mice. Endocrinology 2001, 15: 125-135. PMID: 11145744, DOI: 10.1210/mend.15.1.0588.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaBlotting, WesternCalciumCells, CulturedFemaleFluorescent Antibody TechniqueGonadotropin-Releasing HormoneIsoenzymesMaleMiceMice, KnockoutMicroscopy, FluorescenceMuscle, Smooth, VascularPituitary GlandSignal TransductionSomatostatinThyrotropin-Releasing HormoneType C PhospholipasesConceptsWild-type miceKnockout micePituitary cellsSmooth muscle cellsCalcium responseIntracellular calciumMuscle cellsAortic smooth muscle cellsThyrotropin-Releasing HormoneCalcium-mobilizing actionGonadotropin-Releasing HormoneInhibited influxMicroM TRHExtracellular calciumSomatostatinPituitary tissueKnockout animalsMiceHormoneSignal pathwayPLCbeta1Phospholipase CbetaPLCbeta3CalciumGalphaq/11
2000
Roles of phospholipid signaling in chemoattractant-induced responses
Wu D, Huang C, Jiang H. Roles of phospholipid signaling in chemoattractant-induced responses. Journal Of Cell Science 2000, 113: 2935-2940. PMID: 10934033, DOI: 10.1242/jcs.113.17.2935.Peer-Reviewed Original ResearchRoles of PLC-β2 and -β3 and PI3Kγ in Chemoattractant-Mediated Signal Transduction
Li Z, Jiang H, Xie W, Zhang Z, Smrcka A, Wu D. Roles of PLC-β2 and -β3 and PI3Kγ in Chemoattractant-Mediated Signal Transduction. Science 2000, 287: 1046-1049. PMID: 10669417, DOI: 10.1126/science.287.5455.1046.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesChemokine CCL4Chemotactic FactorsChemotaxis, LeukocyteImmunoglobulin lambda-ChainsIsoenzymesMacrophage Inflammatory ProteinsMiceNeutrophil InfiltrationNeutrophilsN-Formylmethionine Leucyl-PhenylalaninePeritonitisPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhospholipase C betaPhosphorylationSignal TransductionSkin UlcerSuperoxidesType C PhospholipasesClimbing fiber synapse elimination during postnatal cerebellar development requires signal transduction involving Gαq and phospholipase Cβ4
Hashimoto K, Watanabe M, Kurihara H, Offermanns S, Jiang H, Wu Y, Jun K, Shin H, Inoue Y, Wu D, Simon M, Kano M. Climbing fiber synapse elimination during postnatal cerebellar development requires signal transduction involving Gαq and phospholipase Cβ4. Progress In Brain Research 2000, 124: 31-48. PMID: 10943115, DOI: 10.1016/s0079-6123(00)24006-5.Peer-Reviewed Original ResearchAge FactorsAnimalsAtaxiaCell SizeCerebellumExcitatory Postsynaptic PotentialsGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding ProteinsIsoenzymesMiceMice, Neurologic MutantsMicroscopy, ElectronNerve FibersOrgan Culture TechniquesPhospholipase C betaPurkinje CellsRNA, MessengerSignal TransductionSynapsesType C Phospholipases
1999
Genetic alteration of phospholipase C β3 expression modulates behavioral and cellular responses to μ opioids
Xie W, Samoriski G, McLaughlin J, Romoser V, Smrcka A, Hinkle P, Bidlack J, Gross R, Jiang H, Wu D. Genetic alteration of phospholipase C β3 expression modulates behavioral and cellular responses to μ opioids. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10385-10390. PMID: 10468617, PMCID: PMC17897, DOI: 10.1073/pnas.96.18.10385.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCalcium ChannelsCell MembraneEnkephalin, Ala(2)-MePhe(4)-Gly(5)-EnkephalinsGanglia, SpinalGene Expression RegulationGene Expression Regulation, EnzymologicIsoenzymesMembrane PotentialsMiceMice, KnockoutMorphineNeurons, AfferentPainPhospholipase C betaReceptors, Opioid, deltaReceptors, Opioid, kappaReceptors, Opioid, muType C PhospholipasesConceptsBeta3-null miceDorsal root ganglion neuronsPLC-beta3Mu-opioid responseOpioid receptor numberSpecific mu agonistPrimary sensory neuronsPhospholipase CDelta-opioid receptorsWild-type neuronsBeta3-deficient miceProtein kinase CDAMGO responseMicro opioidsOpioid responseOpioid regulationMu agonistsOpioid sensitivityGanglion neuronsΜ-opioidOpioid receptorsReceptor numberSensory neuronsKinase CNumber of intracellular
1998
Phospholipase Cβ4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum
Kano M, Hashimoto K, Watanabe M, Kurihara H, Offermanns S, Jiang H, Wu Y, Jun K, Shin H, Inoue Y, Simon M, Wu D. Phospholipase Cβ4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 15724-15729. PMID: 9861037, PMCID: PMC28111, DOI: 10.1073/pnas.95.26.15724.Peer-Reviewed Original ResearchConceptsCF synapse eliminationSynapse eliminationType 1 metabotropic glutamate receptorFiber synapse eliminationMultiple CF innervationMetabotropic glutamate receptorsPurkinje cell synapsesCF innervationCell synapsesGlutamate receptorsCaudal cerebellumCerebellar vermisRostral portionClimbing fibresRostral cerebellumCerebellar histologyPurkinje cellsSynapse formationMutant miceLocomotor ataxiaBasic electrophysiologyPhospholipase C isoformsPhospholipase Cβ4CerebellumDevelopmental eliminationIdentification of a Structural Element in Phospholipase C β2 That Interacts with G Protein βγ Subunits*
Sankaran B, Osterhout J, Wu D, Smrcka A. Identification of a Structural Element in Phospholipase C β2 That Interacts with G Protein βγ Subunits*. Journal Of Biological Chemistry 1998, 273: 7148-7154. PMID: 9507029, DOI: 10.1074/jbc.273.12.7148.Peer-Reviewed Original Research
1997
Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*
Xie W, Jiang H, Wu Y, Wu D. Two Basic Amino Acids in the Second Inner Loop of the Interleukin-8 Receptor Are Essential for Gα16 Coupling*. Journal Of Biological Chemistry 1997, 272: 24948-24951. PMID: 9312098, DOI: 10.1074/jbc.272.40.24948.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDArginineBinding SitesCOS CellsGTP-Binding ProteinsInterleukin-8KineticsLysineMolecular Sequence DataMutagenesis, Site-DirectedPhosphatidylinositol Diacylglycerol-LyaseProtein Structure, SecondaryReceptors, InterleukinReceptors, Interleukin-8ARecombinant Fusion ProteinsTransfectionType C PhospholipasesRoles of phospholipase C β2 in chemoattractant-elicited responses
Jiang H, Kuang Y, Wu Y, Xie W, Simon M, Wu D. Roles of phospholipase C β2 in chemoattractant-elicited responses. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 7971-7975. PMID: 9223297, PMCID: PMC21539, DOI: 10.1073/pnas.94.15.7971.Peer-Reviewed Original Research
1996
Phospholipase C β4 is involved in modulating the visual response in mice
Jiang H, Lyubarsky A, Dodd R, Vardi N, Pugh E, Baylor D, Simon M, Wu D. Phospholipase C β4 is involved in modulating the visual response in mice. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 14598-14601. PMID: 8962098, PMCID: PMC26179, DOI: 10.1073/pnas.93.25.14598.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsIsoenzymesMiceMice, KnockoutMutationPhospholipase C betaRetinaType C PhospholipasesVisual PathwaysConceptsPLC-beta 4Beta 4Phospholipase C beta 4Cell-cell interactionsC beta 4Phototransduction cascadePLC-betaRod photoreceptorsPhysiological significanceMouse linesLateral geniculate nucleusPhospholipase C β4Wild-type littermatesElectroretinographic resultsVisual functionGeniculate nucleusLittermate controlsSuperior colliculusRod aRetinal cellsBehavioral testsLight microscopyB-wave componentVisual responsesMicePertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*
Jiang H, Kuang Y, Wu Y, Smrcka A, Simon M, Wu D. Pertussis Toxin-sensitive Activation of Phospholipase C by the C5a and fMet-Leu-Phe Receptors*. Journal Of Biological Chemistry 1996, 271: 13430-13434. PMID: 8662841, DOI: 10.1074/jbc.271.23.13430.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDCell LineEnzyme ActivationGTP-Binding ProteinsMolecular Sequence DataPertussis ToxinReceptor, Anaphylatoxin C5aReceptors, ComplementReceptors, Formyl PeptideReceptors, ImmunologicReceptors, PeptideSignal TransductionTransfectionType C PhospholipasesVirulence Factors, BordetellaConceptsPLC-beta3PTX-sensitive G proteinsG proteinsFMet-LeuGbetagamma subunitsCOS-7 cellsPhospholipase CPLC-beta2Receptor-mediated PLC activationPTX-sensitive mannerFMLP-mediated activationPertussis toxinPhe receptorCell activationGi proteinsChemoattractant receptorsReceptorsPLC activationC5aBeta2Gq classActivationBeta3Betagamma subunitsSignal transduction pathwaysIdentification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNASelective G Protein Coupling by C-C Chemokine Receptors (∗)
Kuang Y, Wu Y, Jiang H, Wu D. Selective G Protein Coupling by C-C Chemokine Receptors (∗). Journal Of Biological Chemistry 1996, 271: 3975-3978. PMID: 8626727, DOI: 10.1074/jbc.271.8.3975.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAvian ProteinsCell LineChemokine CCL2Chlorocebus aethiopsCloning, MolecularDNA PrimersDNA-Binding ProteinsGTP-Binding ProteinsHumansKineticsMonocytesPertussis ToxinPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineReceptors, CytokineRecombinant ProteinsTransfectionType C PhospholipasesVirulence Factors, BordetellaZinc FingersConceptsC chemokine receptor 1C chemokine receptorChemokine receptorsBroad spectrum anti-inflammatory drugsPertussis toxin-sensitive mannerChemokine receptor 1MCP-1 receptorAnti-inflammatory drugsG protein coupling specificityToxin-sensitive mannerGq classG protein couplingAccumulation of inositolPhospholipase CC chemokinesT cellsInflammatory reactionCotransfected COS-7 cellsGi proteinsReceptor 1Major mediatorC-terminal intracellular domainG beta gammaProtein couplingReceptors
1995
Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗)
Wu D, Kuang Y, Wu Y, Jiang H. Selective Coupling of β2-Adrenergic Receptor to Hematopoietic-specific G Proteins (∗). Journal Of Biological Chemistry 1995, 270: 16008-16010. PMID: 7608160, DOI: 10.1074/jbc.270.27.16008.Peer-Reviewed Original ResearchConceptsG alpha 16G alpha 15G alpha 11G alpha qG alpha 14Alpha 14Alpha 11Alpha qPhospholipase CAlpha 15G beta gammaAR agonist isoprenalineAlpha 16Norepinephrine-induced increaseNorepinephrine-induced activationG proteinsActivation of PLCCOS-7 cellsReceptor-mediated activationAccumulation of inositolConcentration-dependent mannerAntagonist propranololCotransfected cellsΒ2-adrenergic receptorAgonist isoprenaline
1994
Activation of phospholipase C beta 4 by heterotrimeric GTP-binding proteins.
Jiang H, Wu D, Simon M. Activation of phospholipase C beta 4 by heterotrimeric GTP-binding proteins. Journal Of Biological Chemistry 1994, 269: 7593-7596. PMID: 8125982, DOI: 10.1016/s0021-9258(17)37328-3.Peer-Reviewed Original ResearchConceptsPhospholipase C beta 4Beta 4PLC-beta 4C beta 4Gq classBeta 2 gamma 2Beta 1 gamma 2G alpha 15Alpha subunitG alpha 11G alpha qG alpha 16Gamma 2G beta gamma subunitsG alpha 14G beta 1 gamma 2Alpha 14Beta 1 gamma 1Beta gamma subunitsBeta 1 isoformAlpha 11Alpha qAlpha 15Heterotrimeric GTPGamma subunits
1993
G Protein-Coupled Signal Transduction Pathways for Interleukin-8
Wu D, LaRosa G, Simon M. G Protein-Coupled Signal Transduction Pathways for Interleukin-8. Science 1993, 261: 101-103. PMID: 8316840, DOI: 10.1126/science.8316840.Peer-Reviewed Original ResearchConceptsIL-8 receptorsInterleukin-8Free beta gamma subunitsPertussis toxin-sensitive G proteinToxin-sensitive G proteinIL-8 actsPhospholipase CSpecific heterotrimeric G proteinsAnti-inflammatory agentsG alpha 15G proteinsG alpha 11G alpha qG alpha 16G protein GiBeta 2 isoformG alpha 14Inflammatory responseAlpha 14Beta gamma subunitsProtein GiAlpha 11Alpha qMajor mediatorAlpha 15
1992
Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family.
Wu D, Katz A, Lee C, Simon M. Activation of phospholipase C by alpha 1-adrenergic receptors is mediated by the alpha subunits of Gq family. Journal Of Biological Chemistry 1992, 267: 25798-25802. PMID: 1334487, DOI: 10.1016/s0021-9258(18)35680-1.Peer-Reviewed Original ResearchConceptsG alpha 11G alpha 16G alpha qG alpha 14Alpha 14Phospholipase C beta 1C beta 1Alpha 11Alpha qBeta 1C receptorAlpha 16Cos-7 cellsPhospholipase CAlpha 1Subtypes of alphaRegulatory protein alpha subunitFunctional couplingG alpha subunitsAlpha subunitInositol phosphate releaseG alpha q/G alpha 11Synergistic responseReceptor subtypesSame assay systemSubunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C
Katz A, Wu D, Simon M. Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase C. Nature 1992, 360: 686-689. PMID: 1465134, DOI: 10.1038/360686a0.Peer-Reviewed Original ResearchConceptsΑ-subunitG proteinsExchange of GDPVitro reconstitution experimentsReceptor-mediated releaseDifferent G proteinsFree GβγPhospholipase C activationGβγ complexG proteins resultsGα subunitsHL-60 cellsGβγ subunitsTransfection assaysPertussis toxinΓ subunitProtein resultsGβγReconstitution experimentsDifferent isoformsSpecific receptorsΒ2 isoformPhospholipase CΒ1 isoformsSubunits