2012
Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response
Ramírez C, Díaz-Toro Y, Tellez J, Castilho TM, Rojas R, Ettinger NA, Tikhonova I, Alexander ND, Valderrama L, Hager J, Wilson ME, Lin A, Zhao H, Saravia NG, McMahon-Pratt D. Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response. PLOS Neglected Tropical Diseases 2012, 6: e1866. PMID: 23145196, PMCID: PMC3493378, DOI: 10.1371/journal.pntd.0001866.Peer-Reviewed Original ResearchConceptsMRNA abundance profilesMonocyte-derived macrophagesRegulation of genesDistinct biologic responsesAbundance profilesL. panamensisDifferential gene expressionEarly time pointsMacrophage responseEarly macrophage responseImmune responseLeishmania speciesHuman monocyte-derived macrophagesMicroarray evidenceOutset of infectionGene regulationActivation of PKCHuman macrophage responseGene expressionLeishmania panamensis infectionTime pointsCellular responsesAdaptive immune responsesG proteinsEarly inflammatory response
2006
Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis
Carrillo E, Ahmed S, Goldsmith-Pestana K, Nieto J, Osorio Y, Travi B, Moreno J, McMahon-Pratt D. Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis. Vaccine 2006, 25: 1534-1543. PMID: 17178178, PMCID: PMC2571115, DOI: 10.1016/j.vaccine.2006.10.036.Peer-Reviewed Original ResearchConceptsSoluble Leishmania antigenAmastigote antigensImmune responseAmerican cutaneous leishmaniasis patientsTh1-like immune responseSoluble leishmanial antigenCutaneous leishmaniasis patientsIL-4 mRNAAppropriate immune responseCanine visceral leishmaniasisElicit appropriate immune responsesIdentification of LeishmaniaGeneral vaccinesLeishmanial antigensIL-10Leishmania antigenLeishmaniasis patientsSLA antigensTNF-alphaDisease manifestationsIFN-gammaMurine modelMouse modelVisceral leishmaniasisLeishmania infantum
2004
Leishmanial Amastigote Antigen P‐2 Induces Major Histocompatibility Complex Class II‐Dependent Natural Killer‐Cell Reactivity in Cells from Healthy Donors
Nylén S, Maasho K, McMahon‐Pratt D, Akuffo H. Leishmanial Amastigote Antigen P‐2 Induces Major Histocompatibility Complex Class II‐Dependent Natural Killer‐Cell Reactivity in Cells from Healthy Donors. Scandinavian Journal Of Immunology 2004, 59: 294-304. PMID: 15030581, DOI: 10.1111/j.0300-9475.2004.01388.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ProtozoanCD8-Positive T-LymphocytesFetal BloodHistocompatibility Antigens Class IIHumansInterferon-gammaInterleukin-10Killer Cells, NaturalLeishmaniaLeishmaniasisLymphocyte ActivationProtozoan VaccinesReceptors, IgGReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsHealthy donorsLeishmaniasis patientsAmerican cutaneous leishmaniasis patientsClass IIMajor histocompatibility complex class IIMajor histocompatibility complex classHistocompatibility complex class IIClass II antibodiesCutaneous leishmaniasis patientsInterleukin-10 productionNatural killer cellsProtective immune responseInterferon-gamma productionIFN-gamma responsesMHC class IIHistocompatibility complex classHealthy adult donorsDevelopment of vaccinesAdherent cell populationAmastigote antigensNatural killerKiller cellsCytokine productionCell reactivityLeishmania infection
1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1993
Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction
Traub-Cseko Y, Duboise M, Boukai L, McMahon-Pratt D. Identification of two distinct cysteine proteinase genes of Leishmania pifanoi axenic amastigotes using the polymerase chain reaction. Molecular And Biochemical Parasitology 1993, 57: 101-115. PMID: 8426606, DOI: 10.1016/0166-6851(93)90248-v.Peer-Reviewed Original ResearchConceptsC-terminal extensionCysteine proteinase geneComparative sequence analysisCysteine proteinasesActive site residuesAmastigote cysteine proteinasesMexicana complexChromosome locationGenomic arrangementDistinct genesCDNA sequenceSequence dataDNA sequencesTrypanosoma bruceiProtein sequencesBiological functionsProteinase geneSite residuesDegenerate primersSelective conservationLysosomal organellesGlycine residueGenomic copiesSequence analysisLeishmania mexicana complex
1990
Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.
LeBowitz J, Coburn C, McMahon-Pratt D, Beverley S. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 9736-9740. PMID: 2124701, PMCID: PMC55248, DOI: 10.1073/pnas.87.24.9736.Peer-Reviewed Original ResearchConceptsExpression vectorCis-acting elementsTrypanosomatid protozoan parasitesRNA editingSurface antigen geneMolecular paradigmProcessing eventsParasite proteinsGenetic analysisLeishmania expression vectorExpression sitesPathogenic speciesNormal transcriptMembrane glycoproteinsEscherichia coliGenesImmunoblot analysisProtozoan parasiteProteinAntigen geneColony assayLeishmania majorFamily membersL. amazonensisKilobasesMolecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysis