2001
Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8
Colmenares M, Tiemeyer M, Kima P, McMahon-Pratt D. Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8. Infection And Immunity 2001, 69: 6776-6784. PMID: 11598050, PMCID: PMC100055, DOI: 10.1128/iai.69.11.6776-6784.2001.Peer-Reviewed Original ResearchConceptsAntigen PFurther vaccine studiesSignificant T cell activationT cell activationWestern blot analysisProtective immunityVaccine studiesApolipoprotein EMurine modelCutaneous leishmaniasisSignificant protectionComparable protectionImmunodominant componentAntigenic glycolipidsImmunity studiesAntigenCysteine proteinasesGlycolipid componentBiochemical analysisBiological characterizationSodium dodecyl sulfate-polyacrylamide gel electrophoresisDodecyl sulfate-polyacrylamide gel electrophoresisSulfate-polyacrylamide gel electrophoresisInfectionLeishmaniasis
1997
Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
Franco A, MC Machado G, Naiff R, Moreira C, McMahon-Pratt D, Grimaldi G. Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies. Memórias Do Instituto Oswaldo Cruz 1997, 92: 63-68. DOI: 10.1590/s0074-02761997000100013.Peer-Reviewed Original ResearchMonoclonal antibodiesNumber of mAbsWestern blot analysisSpecific monoclonal antibodiesImmunological assay systemsEpitope densityAntigenic determinantsAntigenic groupsMAbsAntigenic diversityBlot analysisELISAAntibodiesImmunofluorescenceAssay systemParasitesE. schaudinniClone EMolecular formsGeographic areasLatter parasiteReactivity patternsL. equatorensis
1992
Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex
McMahon-Pratt D, Traub-Cseko Y, Lohman K, Rogers D, Beverley S. Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex. Molecular And Biochemical Parasitology 1992, 50: 151-160. PMID: 1542309, DOI: 10.1016/0166-6851(92)90252-f.Peer-Reviewed Original ResearchConceptsGP46/MGene familyGlycoprotein gene familyMolecular karyotype analysisBraziliensis complexLeishmania braziliensis complexSpeciation eventsTrypanosomatid speciesPhylogenetic analysisCrithidia fasciculataLeishmania tarentolaeDevelopmental cycleWestern blot analysisLeishmania mexicana complexMembrane glycoproteinsKaryotype analysisBlot analysisL. braziliensis complexProtein expressionPolyclonal antiserumMexicana complexSpeciesL. panamensisLeishmania majorL. braziliensis
1989
Monoclonal Antibodies Specific for Members of the Genus Endotrypanum
LOPES A, McMAHON‐PRATT D. Monoclonal Antibodies Specific for Members of the Genus Endotrypanum. Journal Of Eukaryotic Microbiology 1989, 36: 354-361. PMID: 2504911, DOI: 10.1111/j.1550-7408.1989.tb05526.x.Peer-Reviewed Original Research
1984
Production and characterization of species-specific monoclonal antibodies against Leishmania donovani for immunodiagnosis.
Jaffe C, Bennett E, Grimaldi G, McMahon-Pratt D. Production and characterization of species-specific monoclonal antibodies against Leishmania donovani for immunodiagnosis. The Journal Of Immunology 1984, 133: 440-7. PMID: 6373932, DOI: 10.4049/jimmunol.133.1.440.Peer-Reviewed Original ResearchConceptsMonoclonal antibodiesVisceral leishmaniasisSpecies-specific monoclonal antibodiesL. donovaniAntibody DAntigenic determinantsLeishmania donovaniKala-azar serumWestern blot analysisSpecies of LeishmaniaSerologic assaysEpidemiologic studiesClinical diagnosisWestern blotAntigenic moleculesProtein antigensRadioimmunoprecipitation analysisAntibodiesLeishmaniasisLeishmania speciesLeishmania stocksDonovaniMonoclonal antibody DBlot analysisAntigen