2012
The Abl and Arg Kinases Mediate Distinct Modes of Phagocytosis and Are Required for Maximal Leishmania Infection
Wetzel DM, McMahon-Pratt D, Koleske AJ. The Abl and Arg Kinases Mediate Distinct Modes of Phagocytosis and Are Required for Maximal Leishmania Infection. Molecular And Cellular Biology 2012, 32: 3176-3186. PMID: 22665498, PMCID: PMC3434515, DOI: 10.1128/mcb.00086-12.Peer-Reviewed Original ResearchConceptsComplement receptor 3Leishmania infectionIgG-coated beadsMurine cutaneous leishmaniasisPotential therapeutic targetLeishmania uptakeVisceral diseaseObligate intracellular parasitesCutaneous leishmaniasisTherapeutic targetFc receptorsAmastigote uptakeTreatment resultsReceptor 3Small lesionsInfection severityLeishmania amazonensisKinase inhibitorsIntracellular parasitesBead phagocytosisPhagocytosisReceptorsC3biInfectionLeishmaniasis
1996
Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms
Hodgkinson V, Soong L, Duboise S, McMahon-Pratt D. Leishmania amazonensis:Cultivation and Characterization of Axenic Amastigote-like Organisms. Experimental Parasitology 1996, 83: 94-105. PMID: 8654556, DOI: 10.1006/expr.1996.0053.Peer-Reviewed Original ResearchConceptsAmastigote-like formsNorthern blot analysisDevelopmental biologyContinual cultureAxenic cultureMolecular biologyTransformation of promastigotesOrganismsDNA probesGeneration timeCultured amastigotesAxenic cultivationBlot analysisExpression levelsSpecies of LeishmaniaSpeciesProtein reactiveBiologyDegrees CFirst reportPeriod of growthAmastigotesPromastigotesLeishmania amazonensisCultivation
1991
Biochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis
Rivas L, Kahl L, Manson K, McMahon-Pratt D. Biochemical characterization of the protective membrane glycoprotein GP46/M-2 of Leishmania amazonensis. Molecular And Biochemical Parasitology 1991, 47: 235-243. PMID: 1719417, DOI: 10.1016/0166-6851(91)90183-7.Peer-Reviewed Original ResearchConceptsGP46/MCarbohydrate side chainsDNA-derived protein sequenceLipid anchorSpecies-specific epitopesProtein sequencesSide chainsBiochemical characterizationLeishmania amazonensisProteinBiochemical featuresPolypeptide portionSurface membraneMonoclonal antibodiesPolypeptideAmazonensisMoleculesEpitopesAnchorsSequenceMembraneDigestionAntibodiesChainPromastigotesLeishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease.
Barral A, Pedral-Sampaio D, Grimaldi G, Momen H, McMahon-Pratt D, de Jesus A, Almeida R, Badaro R, Barral-Netto M, Carvalho E, Johnson W. Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease. American Journal Of Tropical Medicine And Hygiene 1991, 44: 536-46. PMID: 2063957, DOI: 10.4269/ajtmh.1991.44.536.Peer-Reviewed Original ResearchConceptsMucocutaneous leishmaniasisCutaneous leishmaniasisClinical presentationVisceral leishmaniasisCases of CLMonoclonal antibodiesLeishmania amazonensisCases of DCLL. amazonensis infectionDifferent clinical presentationsDifferent clinical formsDifferent Leishmania speciesAmazonensis infectionClinical groundsIndistinguishable clinical presentationDermal leishmaniasisSpecific monoclonal antibodiesClinical formsClinical diseaseLeishmanial diseaseL. chagasiWide spectrumLeishmania referenceLeishmaniasisL. amazonensis
1990
Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.
Lohman K, Langer P, McMahon-Pratt D. Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 8393-8397. PMID: 2236047, PMCID: PMC54962, DOI: 10.1073/pnas.87.21.8393.Peer-Reviewed Original ResearchConceptsGP46/MDNA sequence dataHydrophobic leader peptideCarboxyl-terminal domainMetal-binding motifGlycosyl-phosphatidylinositol anchorAmino-terminal portionProtozoan Leishmania amazonensisSouthern blot analysisGene familyMature proteinPutative attachment siteMolecular cloningProtein comprisesRepetitive sequencesSequence dataCarboxyl terminusCysteine residuesProtein sequencesLeader peptideMembrane locationBinding motifLeishmania amazonensisMembrane glycoproteinsBlot analysis
1989
Identification, using isoenzyme electrophoresis and monoclonal antibodies, of Leishmania isolated from humans and wild animals of Ecuador.
Mimori T, Grimaldi G, Kreutzer R, Gomez E, McMahon-Pratt D, Tesh R, Hashiguchi Y. Identification, using isoenzyme electrophoresis and monoclonal antibodies, of Leishmania isolated from humans and wild animals of Ecuador. American Journal Of Tropical Medicine And Hygiene 1989, 40: 154-8. PMID: 2919726, DOI: 10.4269/ajtmh.1989.40.154.Peer-Reviewed Original Research
1988
Membrane glycoprotein M-2 protects against Leishmania amazonensis infection
Champsi J, McMahon-Pratt D. Membrane glycoprotein M-2 protects against Leishmania amazonensis infection. Infection And Immunity 1988, 56: 3272-3279. PMID: 3182080, PMCID: PMC259734, DOI: 10.1128/iai.56.12.3272-3279.1988.Peer-Reviewed Original ResearchConceptsMouse strainsComplete protectionBALB/c strainLeishmania amazonensis infectionT cell immunityProtective immune responseOnset of infectionC. parvumAmazonensis infectionInfecting doseProtective immunityChallenge infectionC57BL/6 miceAntibody responseCBA miceComplete adjuvantEffective adjuvantImmune responseImmunized animalsLog phase promastigotesLevel of protectionL. amazonensisInfectionC strainLeishmania amazonensis