Featured Publications
Coronavirus multiplication: locations of genes for virion proteins on the avian infectious bronchitis virus genome
Stern DF, Sefton BM. Coronavirus multiplication: locations of genes for virion proteins on the avian infectious bronchitis virus genome. Journal Of Virology 1984, 50: 22-29. PMID: 6321790, PMCID: PMC255576, DOI: 10.1128/jvi.50.1.22-29.1984.Peer-Reviewed Original ResearchConceptsVirion proteinsRNA AMessenger-dependent rabbit reticulocyte lysateTwo-dimensional tryptic peptide mappingPolypeptide of caSmall membrane proteinsLocation of genesAvian coronavirus infectious bronchitis virusNucleocapsid proteinRabbit reticulocyte lysateMajor virion proteinsCoronavirus infectious bronchitis virusCell-free translationInfectious bronchitis virus genomeSmall RNAsTryptic peptide mappingMembrane proteinsProtein p23Reticulocyte lysateSpecific mRNAsRNA CViral mRNAsCore polypeptidesMessenger activityVirus genome
2004
Establishment of a Cell-Free System to Study the Activation of Chk2
Xu X, Stern DF. Establishment of a Cell-Free System to Study the Activation of Chk2. Methods In Molecular Biology 2004, 280: 165-174. PMID: 15187252, DOI: 10.1385/1-59259-788-2:165.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxia Telangiectasia Mutated ProteinsCell Cycle ProteinsCell-Free SystemCheckpoint Kinase 2DNA DamageDNA-Binding ProteinsGenetic VectorsHumansImmunoblottingPlasmidsPrecipitin TestsProtein BiosynthesisProtein Serine-Threonine KinasesRabbitsReticulocytesTranscription, GeneticTriticumTumor Suppressor ProteinsConceptsActivation of Chk2Cell-free systemVitro transcription/translation systemTranscription/translation systemCheckpoint kinase Chk2Rabbit reticulocyte lysateWheat germ extractKinase Chk2Identification of cofactorsReticulocyte lysateChk2Germ extractDNA damageTranslation systemActivationKinaseCofactorProteinATRLysatesPathway