2000
Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides
DiGiovanna M, Roussel R, Stern D. Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides. Current Protocols In Molecular Biology 2000, 50: 18.6.1-18.6.19. PMID: 18265171, DOI: 10.1002/0471142727.mb1806s50.BooksConceptsAnti-phosphopeptide antibodiesIndividual phosphorylation sitesAnalysis of phosphoproteinsAnti-phosphoamino acid antibodiesPhosphorylation sitesUnphosphorylated proteinTyrosine phosphorylated peptidesCognate proteinPhosphorylated stateParticular proteinAffinity matrixProteinUnique specificityPhosphoproteinFunctional stateDifferential isolationSuch reagentsPhosphotyrosinePeptidesSupport protocolSpeciesAbundanceProduction of antibodiesConventional antibodies
1991
TPA inhibits the tyrosine kinase activity of the neu protein in vivo and in vitro.
Cao H, Decker S, Stern DF. TPA inhibits the tyrosine kinase activity of the neu protein in vivo and in vitro. Oncogene 1991, 6: 705-11. PMID: 1675782.Peer-Reviewed Original ResearchConceptsImmune complex kinase assayReceptor-like proteinTyrosine kinase activityProtein kinase CThreonine phosphorylationThreonine residuesTransmembrane domainKinase assaysTyrosine phosphorylationKinase activityAntiphosphotyrosine antibodyIncubation of cellsKinase CPhosphorylationPoint mutationsProteinNeu/Neu proteinLabeling experimentsSerineP185PhosphotyrosineTPAOncogenicMutations