2021
Polycomb complexes redundantly maintain epidermal stem cell identity during development
Cohen I, Bar C, Liu H, Valdes VJ, Zhao D, Galbo PM, Silva JM, Koseki H, Zheng D, Ezhkova E. Polycomb complexes redundantly maintain epidermal stem cell identity during development. Genes & Development 2021, 35: 354-366. PMID: 33602871, PMCID: PMC7919412, DOI: 10.1101/gad.345363.120.Peer-Reviewed Original ResearchConceptsFunctional redundancyTranscription factorsRepressive chromatin domainsStem cell identityEpidermal stem cellsStrong derepressionEpidermal identityPolycomb complexesChromatin domainsDevelopmental regulatorsIdentity genesPRC2 functionGene repressionGenomic bindingCell identityMolecular dissectionEpidermal progenitorsLineage identityPRC2PRC1Ectopic expressionSevere defectsEpidermal stratificationPhysiological significanceStem cells
2019
Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression
Bar C, Cohen I, Zhao D, Pothula V, Litskevitch A, Koseki H, Zheng D, Ezhkova E. Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression. Cell Reports 2019, 28: 257-266.e5. PMID: 31269445, PMCID: PMC6921245, DOI: 10.1016/j.celrep.2019.06.011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBody PatterningCell CycleChromatin ImmunoprecipitationChromatin Immunoprecipitation SequencingCyclin-Dependent Kinase Inhibitor p16Epigenesis, GeneticEpitheliumGene Expression Regulation, DevelopmentalGene OntologyHedgehog ProteinsMiceMice, KnockoutMicroscopy, Electron, ScanningPolycomb Repressive Complex 1Polycomb Repressive Complex 2RNA-SeqSignal TransductionTaste BudsTongueConceptsGene expressionPolycomb Repressive Complex 1Spatial gene expression patternsRepressive Complex 1Gene expression patternsCell gene expressionChromatin regulatorsTissue patterningSonic hedgehog expressionEpigenetic regulationNiche structureExpression patternsCell genesEpithelial progenitorsHedgehog expressionShhEctopic ShhTaste cellsPapilla structuresTissue patternsExpressionCellsPatterningProper maintenanceComplexes 1
2018
PRC1 preserves epidermal tissue integrity independently of PRC2
Cohen I, Zhao D, Menon G, Nakayama M, Koseki H, Zheng D, Ezhkova E. PRC1 preserves epidermal tissue integrity independently of PRC2. Genes & Development 2018, 33: 55-60. PMID: 30567998, PMCID: PMC6317312, DOI: 10.1101/gad.319939.118.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionEpidermal CellsEpidermisEpigenesis, GeneticGene Expression Regulation, DevelopmentalHistonesMicePolycomb Repressive Complex 1Polycomb Repressive Complex 2Skin DiseasesConceptsAdhesion genesEpidermal tissue integrityLoss of PRC1PRC1 functionChromatin regulatorsEpigenetic regulationMolecular dissectionPRC2PRC1Gene expressionTissue developmentSkin morphogenesisFunctional linkSkin fragility syndromeEpidermal stratificationEpidermal integrityTissue integrityGenesExpressionH3K27me3Fragility syndromeMorphogenesisRegulatorCobindingRegulationPRC1 Fine-tunes Gene Repression and Activation to Safeguard Skin Development and Stem Cell Specification
Cohen I, Zhao D, Bar C, Valdes VJ, Dauber-Decker KL, Nguyen MB, Nakayama M, Rendl M, Bickmore WA, Koseki H, Zheng D, Ezhkova E. PRC1 Fine-tunes Gene Repression and Activation to Safeguard Skin Development and Stem Cell Specification. Cell Stem Cell 2018, 22: 726-739.e7. PMID: 29727681, PMCID: PMC5944606, DOI: 10.1016/j.stem.2018.04.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiocatalysisMiceMice, Inbred StrainsMice, KnockoutPolycomb Repressive Complex 1Polycomb Repressive Complex 2SkinStem CellsConceptsNon-canonical PRC1 complexesStem cell specificationPRC1 complexesCell specificationSkin developmentDiverse rolesRepressive Complex 1Essential chromatin regulatorsPrecise developmental programExpression of genesCanonical PRC1PRC1 bindsPRC1 functionChromatin regulatorsGene repressionActive genesCell identityRepressor functionDevelopmental programSC formationFurther dissectionGenesPRC2PRC1Function studies
2016
Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development
Perdigoto CN, Dauber KL, Bar C, Tsai PC, Valdes VJ, Cohen I, Santoriello FJ, Zhao D, Zheng D, Hsu YC, Ezhkova E. Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development. PLOS Genetics 2016, 12: e1006151. PMID: 27414999, PMCID: PMC4944976, DOI: 10.1371/journal.pgen.1006151.Peer-Reviewed Original ResearchConceptsMerkel cell specificationCell specificationPrimary hair folliclesLoss of PolycombImportance of ShhCell differentiation programHair follicle functionSonic hedgehog (Shh) signalingPRC2 targetsSpecialized keratinocytesEpidermal progenitorsDevelopmental programHair folliclesEpigenetic processesDifferentiation programHedgehog signalingShh ligandMature Merkel cellsShh signalingSkin developmentMurine dorsal skinEpidermal cellsMerkel cellsPRC2 lossCell formation