2024
Characterization of enhancer activity in early human neurodevelopment using Massively Parallel Reporter Assay (MPRA) and forebrain organoids
Capauto D, Wang Y, Wu F, Norton S, Mariani J, Inoue F, Crawford G, Ahituv N, Abyzov A, Vaccarino F. Characterization of enhancer activity in early human neurodevelopment using Massively Parallel Reporter Assay (MPRA) and forebrain organoids. Scientific Reports 2024, 14: 3936. PMID: 38365907, PMCID: PMC10873509, DOI: 10.1038/s41598-024-54302-7.Peer-Reviewed Original ResearchConceptsMassively parallel reporter assaysGene expressionRegulation of gene expressionForebrain organoidsHuman fetal tissuesHigh-throughput assayReporter assayFetal tissuesStem cellsNeurodevelopmentHuman neurodevelopmentActivation signalsEnhanced activityGenesOrganoidsForebrainBrain organoidsAssayBrain
2014
Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination
Boccaccini A, Santopolo S, Capauto D, Lorrai R, Minutello E, Belcram K, Palauqui JC, Costantino P, Vittorioso P. Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination. BMC Plant Biology 2014, 14: 200. PMID: 25064446, PMCID: PMC4222566, DOI: 10.1186/s12870-014-0200-z.Peer-Reviewed Original ResearchConceptsGA INSENSITIVESeed germinationEmbryo developmentDouble mutantGai-t6GERMINATION 1Mutant seedsGa1-3Germination pathwaySingle mutantsRepressorUnexpected involvementMutantsDistinct rolesGerminationGermination propertiesIndependent branchInteractive effectsGERMINATION1DAG1DormancyGenesSeedsPathwayInactivation