2015
Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage
van Dijk D, Dhar R, Missarova AM, Espinar L, Blevins WR, Lehner B, Carey LB. Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage. Nature Communications 2015, 6: 7972. PMID: 26268986, PMCID: PMC4557116, DOI: 10.1038/ncomms8972.Peer-Reviewed Original ResearchConceptsTranscriptional stress responseDNA damageDNA damage responseIsogenic populationsRNA polymerase fidelityDamage responseTranscriptional differencesIsogenic cellsEnvironmental stressCell variabilityStress responseGrowth ratePolymerase fidelityCulture conditionsPolymerase error rateOxidative stressCellsSuch cellsSame environmentTranscriptomeTransposonSubpopulationsTranscriptsGenotypicStress
2013
Promoter Sequence Determines the Relationship between Expression Level and Noise
Carey LB, van Dijk D, Sloot PM, Kaandorp JA, Segal E. Promoter Sequence Determines the Relationship between Expression Level and Noise. PLOS Biology 2013, 11: e1001528. PMID: 23565060, PMCID: PMC3614515, DOI: 10.1371/journal.pbio.1001528.Peer-Reviewed Original ResearchMeSH KeywordsAlcohol DehydrogenaseBacterial ProteinsBase SequenceBinding SitesCation Transport ProteinsEnzyme InductionGene ExpressionGene Expression Regulation, FungalGene LibraryGenes, ReporterKineticsLuminescent ProteinsModels, GeneticPromoter Regions, GeneticProtein BindingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsConceptsTranscriptional burstsUnique transcriptional statesTranscription factor activitySingle target geneExpression levelsMechanism of repressionAbility of cellsTF activityGene expression levelsExtracellular cuesTranscriptional statesTranscriptional regulationPromoter DNAPromoter sequencesSingle TFCell variabilityTarget genesRegulatory mechanismsExpression changesZap1Diverse mechanismsNative targetsFactor activityExpression increasesGenes