2023
Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton
Amiri S, Muresan C, Shang X, Huet-Calderwood C, Schwartz M, Calderwood D, Murrell M. Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton. Nature Communications 2023, 14: 8011. PMID: 38049429, PMCID: PMC10695988, DOI: 10.1038/s41467-023-43612-5.Peer-Reviewed Original ResearchConceptsF-actin architectureStress fibersCortical actinActin cytoskeletonMolecular tension sensorsF-actin stress fibersF-actin cytoskeletonFilamentous actin cytoskeletonMechanical forcesTension sensorCell divisionCytoskeletonCell migrationExtracellular matrixMyosin inhibitionActinDirection of stretchCellsCell axisUniaxial stretchStretchFRET
2022
Organization, dynamics and mechanoregulation of integrin-mediated cell–ECM adhesions
Kanchanawong P, Calderwood DA. Organization, dynamics and mechanoregulation of integrin-mediated cell–ECM adhesions. Nature Reviews Molecular Cell Biology 2022, 24: 142-161. PMID: 36168065, PMCID: PMC9892292, DOI: 10.1038/s41580-022-00531-5.Peer-Reviewed Original ResearchConceptsExtracellular matrixCell-ECM adhesionCell-ECM interactionsLocal extracellular matrixAdhesion maturationAdhesion complexesAnimal cellsBiochemical signalingTransmembrane receptorsAdhesion structuresCell shapeIntegrin familyMolecular natureAge-related dysfunctionAdvanced imaging approachesCharacterization of rearrangementsMechanical forcesSignalingTissue formationAdhesionCytoskeletonMechanoregulationImmune responseImaging approachImproved understanding
2019
Filamin A mediates isotropic distribution of applied force across the actin network
Kumar A, Shutova MS, Tanaka K, Iwamoto DV, Calderwood DA, Svitkina TM, Schwartz MA. Filamin A mediates isotropic distribution of applied force across the actin network. Journal Of Cell Biology 2019, 218: 2481-2491. PMID: 31315944, PMCID: PMC6683746, DOI: 10.1083/jcb.201901086.Peer-Reviewed Original ResearchConceptsTalin tension sensorStress fibersActin networkFilamin ACortical actin networkCortical actin filamentsIntegrin-mediated adhesionActin cytoskeletonFocal adhesionsCortical actinFLNA knockdownActin filamentsTalinKnockdownCell sensingDirection of stretchTension sensorPhysiology of muscleUniaxial stretchForce transmissionCytoskeletonStrainsStretchAdhesionReexpression
2015
Regulation of integrin-mediated adhesions
Iwamoto DV, Calderwood DA. Regulation of integrin-mediated adhesions. Current Opinion In Cell Biology 2015, 36: 41-47. PMID: 26189062, PMCID: PMC4639423, DOI: 10.1016/j.ceb.2015.06.009.Peer-Reviewed Original ResearchConceptsIntegrin-mediated adhesionHeterodimeric transmembrane adhesion receptorsShort cytoplasmic tailTransmembrane adhesion receptorsSpecific intracellular proteinsClustering of integrinsMetazoan developmentActin cytoskeletonExtracellular ligandsCytoplasmic tailIntracellular traffickingExtracellular environmentIntracellular proteinsAdhesion receptorsAdhesive structuresIntegrin receptorsCell membraneRelay signalsIntegrinsEssential roleMechanical forcesCell attachmentAdhesionRecent advancesCytoskeleton
2014
Integrin Cytoplasmic Tail Interactions
Morse EM, Brahme NN, Calderwood DA. Integrin Cytoplasmic Tail Interactions. Biochemistry 2014, 53: 810-820. PMID: 24467163, PMCID: PMC3985435, DOI: 10.1021/bi401596q.Peer-Reviewed Original ResearchConceptsIntegrin-interacting proteinsIntegrin cytoplasmic tailsCell surface adhesion receptorsIntegrin-binding proteinsHeterodimeric cell-surface adhesion receptorsSurface adhesion receptorsExtracellular ligandsMulticellular lifeCytoplasmic tailIntegrin engagementCell motilityExtracellular environmentTransduce chemicalIntegrin activityIntegrin localizationIntracellular proteinsAdhesion receptorsTail interactionsMechanical signalsProteinIntegrinsCellsCytoskeletonLocalizationTrafficking
2012
Filamins in Mechanosensing and Signaling
Razinia Z, Mäkelä T, Ylänne J, Calderwood DA. Filamins in Mechanosensing and Signaling. Annual Review Of Biophysics 2012, 41: 227-246. PMID: 22404683, PMCID: PMC5508560, DOI: 10.1146/annurev-biophys-050511-102252.Peer-Reviewed Original ResearchConceptsPlasma membraneActin filamentsActin-binding proteinsExtracellular matrix connectionsCortical rigidityActin cytoskeletonCellular functionsCell cortexTranscription factorsTransmembrane receptorsAdhesion proteinsCell shapeFilaminIon channelsDiverse arrayFunctional evidenceEssential roleProteinMatrix connectionsPhysical forcesMembraneFilamentsCytoskeletalMechanosensingCytoskeleton
2006
The Molecular Basis of Filamin Binding to Integrins and Competition with Talin
Kiema T, Lad Y, Jiang P, Oxley CL, Baldassarre M, Wegener KL, Campbell ID, Ylänne J, Calderwood DA. The Molecular Basis of Filamin Binding to Integrins and Competition with Talin. Molecular Cell 2006, 21: 337-347. PMID: 16455489, DOI: 10.1016/j.molcel.2006.01.011.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCalpainContractile ProteinsCrystallography, X-RayFilaminsIntegrin beta ChainsMiceMicrofilament ProteinsModels, MolecularMolecular Sequence DataNIH 3T3 CellsNuclear Magnetic Resonance, BiomolecularProtein BindingProtein ConformationProtein Structure, TertiaryRecombinant Fusion ProteinsReproducibility of ResultsSequence Homology, Amino AcidTalinConceptsAdhesion receptorsTalin-dependent integrin activationActin-crosslinking proteinsIntegrin adhesion receptorsHigh-resolution structuresFilamin bindingExtended beta strandActin cytoskeletonIntegrin tailsMultiple transmembraneMolecular basisStrands CBeta strandsDomain interactionsBiochemical signalsIntegrin functionIntegrin activationFilamin ATalinCell membraneTail formsCytoskeletonProteinBinding sitesFilamin
2003
Talin forges the links between integrins and actin
Calderwood DA, Ginsberg MH. Talin forges the links between integrins and actin. Nature Cell Biology 2003, 5: 694-696. PMID: 12894175, DOI: 10.1038/ncb0803-694.Peer-Reviewed Original Research
2000
Integrin cytoplasmic domain-binding proteins
Liu S, Calderwood D, Ginsberg M. Integrin cytoplasmic domain-binding proteins. Journal Of Cell Science 2000, 113: 3563-3571. PMID: 11017872, DOI: 10.1242/jcs.113.20.3563.Peer-Reviewed Original ResearchConceptsDomain-binding proteinCytoplasmic domainCellular proteinsIntegrin cytoplasmic domainActin-binding proteinsMore cellular proteinsCell surface receptorsGene regulationCellular functionsTransduce signalsSignal transductionBiological functionsGene expressionFunctional analysisCell adhesionLarge familySurface receptorsProteinCytoskeletonIntegrin chainsIntegrinsBiological responsesPivotal roleMechanical linkImportant role
1999
The Talin Head Domain Binds to Integrin β Subunit Cytoplasmic Tails and Regulates Integrin Activation*
Calderwood D, Zent R, Grant R, Rees D, Hynes R, Ginsberg M. The Talin Head Domain Binds to Integrin β Subunit Cytoplasmic Tails and Regulates Integrin Activation*. Journal Of Biological Chemistry 1999, 274: 28071-28074. PMID: 10497155, DOI: 10.1074/jbc.274.40.28071.Peer-Reviewed Original ResearchConceptsTalin head domainIntegrin cytoplasmic tailsCytoplasmic tailIntegrin-binding siteHead domainActin cytoskeletonFragment of talinIntegrin beta tailsIntegrin adhesion receptorsSingle point mutationFocal adhesionsCytoplasmic domainCytoplasmic proteinsBeta tailsIntegrin functionIntegrin localizationTalinIntegrin activationIntegrin affinityActin filamentsAdhesion receptorsIntegrin-cytoskeletal interactionsPoint mutationsCytoskeletonStructural mimics