Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40*
Kuszak A, Jacobs D, Gurnev P, Shiota T, Louis J, Lithgow T, Bezrukov S, Rostovtseva T, Buchanan S. Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40*. Journal Of Biological Chemistry 2015, 290: 26204-26217. PMID: 26336107, PMCID: PMC4646270, DOI: 10.1074/jbc.m115.642173.Peer-Reviewed Original ResearchConceptsC-terminal domainPresequence peptideC-terminusMitochondrial outer membraneAmino acidsΒ-barrel domainYeast Candida glabrataOuter membrane complexSubstrate Binding AffinityTranslocase complexNuclear genomeTom40 proteinMitochondrial proteinsProtein transportTom40Substrate recognitionHelical domainOuter membraneMembrane complexCentral subunitN-terminusPlanar lipid membranesTransport functionTerminusChannel conformationEvidence of Distinct Channel Conformations for the Mitochondrial Outer Membrane Translocase Tom40
Kuszak A, Jacobs D, Gurnev P, Lithgow T, Bezrukov S, Rostovtseva T, Buchanan S. Evidence of Distinct Channel Conformations for the Mitochondrial Outer Membrane Translocase Tom40. The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.777.7.Peer-Reviewed Original ResearchN- and C-terminiC-terminiTOM complexSubunit of mitochondrial ATP synthaseMitochondrial ATP synthaseSubstrate binding affinityPresequence peptideC. glabrataSubstrate recognitionMitochondrial proteinsTom40Mitochondrial membraneOuter membraneATP synthaseTruncated formStructural basisSubstrate processingPore-forming subunitConstriction zonePlanar lipid membranesBinding affinityFunctional locationLipid membranesMembranePresequence