Featured Publications
The human malaria parasite Plasmodium falciparum can sense environmental changes and respond by antigenic switching
Schneider V, Visone J, Harris C, Florini F, Hadjimichael E, Zhang X, Gross M, Rhee K, Mamoun C, Kafsack B, Deitsch K. The human malaria parasite Plasmodium falciparum can sense environmental changes and respond by antigenic switching. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2302152120. PMID: 37068249, PMCID: PMC10151525, DOI: 10.1073/pnas.2302152120.Peer-Reviewed Original ResearchConceptsGene switchingGene expressionHistone methyltransferasesHuman malaria parasite Plasmodium falciparumMalaria parasite Plasmodium falciparumS-adenosylmethionineGene expression patternsMulticopy gene familiesVariant surface proteinsParasite Plasmodium falciparumHuman malaria parasiteIntracellular S-adenosylmethioninePrincipal methyl donorEpigenetic controlGene familyActive genesAntigenic switchingGene transcriptionNutrient availabilityExpression patternsMethylation modificationSAM metabolismGenetic modificationAntigenic variationSurface proteins
2020
Palmitoylated Proteins in Plasmodium falciparum‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling
Kilian N, Zhang Y, LaMonica L, Hooker G, Toomre D, Mamoun CB, Ernst AM. Palmitoylated Proteins in Plasmodium falciparum‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling. BioEssays 2020, 42: e1900145. PMID: 32342554, DOI: 10.1002/bies.201900145.Peer-Reviewed Original ResearchConceptsMetabolic labelingHuman malaria parasite Plasmodium falciparumProtein S-palmitoylationImportant post-translational modificationMalaria parasite Plasmodium falciparumComplex cell biologyPost-translational modificationsParasite Plasmodium falciparumTime-consuming generationAsexual developmental stagesPalmitoylated proteinsS-palmitoylationCell biologyP. falciparumTransgenic parasitesExtent of labelingDevelopmental stagesMicroscopy approachSingle-molecule switchingPlasmodium falciparum-infected erythrocytesFalciparum-infected erythrocytesPlasmodium falciparumFalciparumLabelingMicroscopic examination
2006
The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum
Bissati K, Zufferey R, Witola WH, Carter NS, Ullman B, Mamoun C. The plasma membrane permease PfNT1 is essential for purine salvage in the human malaria parasite Plasmodium falciparum. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 9286-9291. PMID: 16751273, PMCID: PMC1482602, DOI: 10.1073/pnas.0602590103.Peer-Reviewed Original ResearchConceptsParasite plasma membraneHuman malaria parasite Plasmodium falciparumMalaria parasite Plasmodium falciparumParasite Plasmodium falciparumPurine salvagePlasma membraneLethal mutantsPlasmodium falciparumInosine transportPurine sourceSpecialized transportersTransgenic parasitesHost enzymesPfNT1Essential nutrientsPotential therapeutic targetParasitesPhysiological conditionsHost purinesInfected erythrocytesSequential pathwaySevere reductionTherapeutic targetP. falciparumHuman erythrocytes
1999
A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum
Mamoun C, Gluzman I, Goyard S, Beverley S, Goldberg D. A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 8716-8720. PMID: 10411941, PMCID: PMC17582, DOI: 10.1073/pnas.96.15.8716.Peer-Reviewed Original ResearchConceptsSelectable markerPlasmodium gene functionHuman malaria parasite Plasmodium falciparumMalaria parasite Plasmodium falciparumP. falciparum genomeBlasticidin S deaminaseParasite Plasmodium falciparumOnly selectable markerHuman malaria pathogenPlasmid copy numberEssential genesFalciparum genomeGene functionIndependent selectable markersPlasmodium falciparumGenomic informationGenetic manipulationGene promoterNeo expressionFunctional analysisPlasmid replicationTn 5Copy numberDihydrofolate reductaseMalaria pathogen