A proteomics approach to understanding protein ubiquitination
Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi S. A proteomics approach to understanding protein ubiquitination. Nature Biotechnology 2003, 21: 921-926. PMID: 12872131, DOI: 10.1038/nbt849.Peer-Reviewed Original ResearchConceptsProtein ubiquitinationProteomic approachUbiquitin conjugatesPolyubiquitin chain topologyAmino acid sequence determinationUbiquitin-protein conjugatesSaccharomyces cerevisiae lysateUbiquitination sitesUnexpected diversityMultidimensional liquid chromatographyProtein modificationLarge-scale analysisLysine residuesSequence determinationUbiquitinationUbiquitinTandem mass spectrometryMass spectrometryProteinGlobal scaleDiversityLysatesResiduesChain topologyTrypsin