2006
mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s
Frias M, Thoreen C, Jaffe J, Schroder W, Sculley T, Carr S, Sabatini D. mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Current Biology 2006, 16: 1865-1870. PMID: 16919458, DOI: 10.1016/j.cub.2006.08.001.Peer-Reviewed Original ResearchConceptsAkt/PKBSerine/threonine kinaseAkt/PKB phosphorylationDistinct multiprotein complexesAssembly of mTORC2Multiprotein complexesThreonine kinaseAlternative splicingPKB phosphorylationMTORC2PKBMammalian targetCell growthMSin1KinaseIsoformsImportant roleSplicingComplexesPhosphorylationRapamycinProteinDifferent signalsRegulationMetabolism
2003
A proteomics approach to understanding protein ubiquitination
Peng J, Schwartz D, Elias J, Thoreen C, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi S. A proteomics approach to understanding protein ubiquitination. Nature Biotechnology 2003, 21: 921-926. PMID: 12872131, DOI: 10.1038/nbt849.Peer-Reviewed Original ResearchConceptsProtein ubiquitinationProteomic approachUbiquitin conjugatesPolyubiquitin chain topologyAmino acid sequence determinationUbiquitin-protein conjugatesSaccharomyces cerevisiae lysateUbiquitination sitesUnexpected diversityMultidimensional liquid chromatographyProtein modificationLarge-scale analysisLysine residuesSequence determinationUbiquitinationUbiquitinTandem mass spectrometryMass spectrometryProteinGlobal scaleDiversityLysatesResiduesChain topologyTrypsin