Featured Publications
An open-access volume electron microscopy atlas of whole cells and tissues
Xu CS, Pang S, Shtengel G, Müller A, Ritter AT, Hoffman HK, Takemura SY, Lu Z, Pasolli HA, Iyer N, Chung J, Bennett D, Weigel AV, Freeman M, van Engelenburg SB, Walther TC, Farese RV, Lippincott-Schwartz J, Mellman I, Solimena M, Hess HF. An open-access volume electron microscopy atlas of whole cells and tissues. Nature 2021, 599: 147-151. PMID: 34616045, PMCID: PMC9004664, DOI: 10.1038/s41586-021-03992-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCells, CulturedDatasets as TopicDrosophila melanogasterFemaleGolgi ApparatusHumansInformation DisseminationInterphaseIslets of LangerhansMaleMiceMicroscopy, Electron, ScanningMicrotubulesNeurogliaNeuronsOpen Access PublishingOrganellesOvarian NeoplasmsRibosomesSynaptic VesiclesT-Lymphocytes, CytotoxicConceptsDrosophila neural tissueWhole cellsThin-section electron microscopyVolume electron microscopyCellular architectureMouse pancreatic isletsCancer cellsEM tomographyCellular structureCellsCellular samplesNeural tissuePancreatic isletsEnhanced signal detectionAtlasBeam-scanning electron microscopyTissueElectron microscopyOpen access dataBiologyImmune cellsSubsequent analysisSEM scanningMicroscopyA connectome and analysis of the adult Drosophila central brain
Scheffer LK, Xu CS, Januszewski M, Lu Z, Takemura SY, Hayworth KJ, Huang GB, Shinomiya K, Maitlin-Shepard J, Berg S, Clements J, Hubbard PM, Katz WT, Umayam L, Zhao T, Ackerman D, Blakely T, Bogovic J, Dolafi T, Kainmueller D, Kawase T, Khairy KA, Leavitt L, Li PH, Lindsey L, Neubarth N, Olbris DJ, Otsuna H, Trautman ET, Ito M, Bates AS, Goldammer J, Wolff T, Svirskas R, Schlegel P, Neace E, Knecht CJ, Alvarado CX, Bailey DA, Ballinger S, Borycz JA, Canino BS, Cheatham N, Cook M, Dreher M, Duclos O, Eubanks B, Fairbanks K, Finley S, Forknall N, Francis A, Hopkins GP, Joyce EM, Kim S, Kirk NA, Kovalyak J, Lauchie S, Lohff A, Maldonado C, Manley EA, McLin S, Mooney C, Ndama M, Ogundeyi O, Okeoma N, Ordish C, Padilla N, Patrick CM, Paterson T, Phillips EE, Phillips EM, Rampally N, Ribeiro C, Robertson MK, Rymer JT, Ryan SM, Sammons M, Scott AK, Scott AL, Shinomiya A, Smith C, Smith K, Smith NL, Sobeski MA, Suleiman A, Swift J, Takemura S, Talebi I, Tarnogorska D, Tenshaw E, Tokhi T, Walsh JJ, Yang T, Horne JA, Li F, Parekh R, Rivlin PK, Jayaraman V, Costa M, Jefferis GS, Ito K, Saalfeld S, George R, Meinertzhagen I, Rubin GM, Hess HF, Jain V, Plaza SM. A connectome and analysis of the adult Drosophila central brain. ELife 2020, 9: e57443. PMID: 32880371, PMCID: PMC7546738, DOI: 10.7554/elife.57443.Peer-Reviewed Original ResearchConceptsCentral brainDrosophila central brainAdult Drosophila Central BrainGenetic reagentsFruit flyComputational compartmentsFly brainExhaustive atlasCell typesAnimal behaviorNeural motifsChemical synapsesDrosophilaSuch large data setsNeural circuitsFliesCompartmentalizationLarge fractionMotifSynapsesCell exampleCompartmentsNeuronsElectrical consequences
2023
Comparative connectomics and escape behavior in larvae of closely related Drosophila species
Zhu J, Boivin J, Pang S, Xu C, Lu Z, Saalfeld S, Hess H, Ohyama T. Comparative connectomics and escape behavior in larvae of closely related Drosophila species. Current Biology 2023, 33: 2491-2503.e4. PMID: 37285846, DOI: 10.1016/j.cub.2023.05.043.Peer-Reviewed Original ResearchConceptsRelated Drosophila speciesDrosophila speciesEscape behaviorDrosophilid speciesRelated speciesMelanogasterSantomeaVentral nerve cordPlausible mechanistic explanationCommon partnerBehavioral traitsMolecular componentsNoxious cuesComparative connectomicsUnderlying neural circuitsSpeciesDownstream partnersMDIVMechanistic explanation
2019
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution
Hayworth K, Peale D, Januszewski M, Knott G, Lu Z, Xu C, Hess H. Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution. Nature Methods 2019, 17: 68-71. PMID: 31740820, DOI: 10.1038/s41592-019-0641-2.Peer-Reviewed Original ResearchConceptsIon beam scanning electron microscopyBeam scanning electron microscopyScanning electron microscopyThree-dimensional electron microscopy techniquesElectron microscopy techniquesIon millingIsotropic resolutionMicroscopy techniquesElectron microscopyThick tissue sectionsResolutionLarge tissue samplesMicroscopyMillingSectionsComparisons between the ON- and OFF-edge motion pathways in the Drosophila brain
Shinomiya K, Huang G, Lu Z, Parag T, Xu CS, Aniceto R, Ansari N, Cheatham N, Lauchie S, Neace E, Ogundeyi O, Ordish C, Peel D, Shinomiya A, Smith C, Takemura S, Talebi I, Rivlin PK, Nern A, Scheffer LK, Plaza SM, Meinertzhagen IA. Comparisons between the ON- and OFF-edge motion pathways in the Drosophila brain. ELife 2019, 8: e40025. PMID: 30624205, PMCID: PMC6338461, DOI: 10.7554/elife.40025.Peer-Reviewed Original Research
2018
A resource for the Drosophila antennal lobe provided by the connectome of glomerulus VA1v
Horne JA, Langille C, McLin S, Wiederman M, Lu Z, Xu CS, Plaza SM, Scheffer LK, Hess HF, Meinertzhagen IA. A resource for the Drosophila antennal lobe provided by the connectome of glomerulus VA1v. ELife 2018, 7: e37550. PMID: 30382940, PMCID: PMC6234030, DOI: 10.7554/elife.37550.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArthropod AntennaeConnectomeDrosophila melanogasterFemaleNerve NetOlfactory Receptor NeuronsSynapses
2015
Synaptic circuits and their variations within different columns in the visual system of Drosophila
Takemura SY, Xu CS, Lu Z, Rivlin PK, Parag T, Olbris DJ, Plaza S, Zhao T, Katz WT, Umayam L, Weaver C, Hess HF, Horne JA, Nunez-Iglesias J, Aniceto R, Chang LA, Lauchie S, Nasca A, Ogundeyi O, Sigmund C, Takemura S, Tran J, Langille C, Le Lacheur K, McLin S, Shinomiya A, Chklovskii DB, Meinertzhagen IA, Scheffer LK. Synaptic circuits and their variations within different columns in the visual system of Drosophila. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 13711-13716. PMID: 26483464, PMCID: PMC4640747, DOI: 10.1073/pnas.1509820112.Peer-Reviewed Original Research