Enhanced FIB-SEM systems for large-volume 3D imaging
Xu CS, Hayworth KJ, Lu Z, Grob P, Hassan AM, García-Cerdán JG, Niyogi KK, Nogales E, Weinberg RJ, Hess HF. Enhanced FIB-SEM systems for large-volume 3D imaging. ELife 2017, 6: e25916. PMID: 28500755, PMCID: PMC5476429, DOI: 10.7554/elife.25916.Peer-Reviewed Original ResearchConceptsFIB-SEM systemFocused Ion Beam Scanning Electron MicroscopyIon beam scanning electron microscopyBeam scanning electron microscopyLong-term system stabilityScanning electron microscopyNovel high-resolution techniqueSystem stabilityFIB-SEMElectron microscopyHigh resolutionHigh-resolution techniquesSmall volumeTransforming FIB-SEMFocused Ion Beam Scanning Electron Microscopy (FIB-SEM) Systems for Large-Volume ConnectomicsConnectomics and Cell BiologyCell biology
Xu C, Pang S, Hayworth K, Hess H. Transforming FIB-SEMFocused Ion Beam Scanning Electron Microscopy (FIB-SEM) Systems for Large-Volume ConnectomicsConnectomics and Cell BiologyCell biology. Neuromethods 2020, 155: 221-243. DOI: 10.1007/978-1-0716-0691-9_12.Peer-Reviewed Original ResearchScanning electron microscopy systemIon beam scanning electron microscopyBeam scanning electron microscopyElectron microscopy systemScanning electron microscopyElectron microscopyLong-term reliabilityScanning electron microscopy technologyRobust imaging platformElectron microscopy technologyConventional FIBMicroscopy systemHigh resolutionFIB-SEMResolution requirementsCell biology researchBoundary conditionsFinal image stackFIBMicroscopy technologyHigh-resolution imagingOrders of magnitudeImaging platformLarge sample volumesImage volumes