ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack
Ritter AT, Shtengel G, Xu CS, Weigel A, Hoffman DP, Freeman M, Iyer N, Alivodej N, Ackerman D, Voskoboinik I, Trapani J, Hess HF, Mellman I. ESCRT-mediated membrane repair protects tumor-derived cells against T cell attack. Science 2022, 376: 377-382. PMID: 35446649, DOI: 10.1126/science.abl3855.Peer-Reviewed Original ResearchConceptsESCRT machineryEndosomal Sorting ComplexCancer-derived cellsESCRT proteinsTarget cellsTumor-derived cellsPlasma membranePerforin poresTransport proteinsMembrane woundsMembrane repairRelease of perforinMachineryCytosolProteinCellsCytolytic attackCytotoxic T lymphocytesESCRTTumor cellsPerforin releaseGranzymesRepairApoptosisCell attackCOPII with ALG2 and ESCRTs control lysosome-dependent microautophagy of ER exit sites
Liao Y, Pang S, Li W, Shtengel G, Choi H, Schaefer K, Xu C, Lippincott-Schwartz J. COPII with ALG2 and ESCRTs control lysosome-dependent microautophagy of ER exit sites. Developmental Cell 2024, 59: 1410-1424.e4. PMID: 38593803, DOI: 10.1016/j.devcel.2024.03.027.Peer-Reviewed Original ResearchEndoplasmic reticulum exit sitesER exit sitesAmino acid starvationPurified recombinant componentsExit siteProtein sortingSecretory pathwayMammalian cellsNutrient stressCellular conditionsEndoplasmic reticulumGiant unilamellar vesiclesTubular outgrowthsESCRTMicroautophagyNutrient stressorsALG2COPIILysosomesPathwayMTOR inhibitionUnilamellar vesiclesRecombinant componentsFocused ion beam scanning electron microscopyIon beam scanning electron microscopy