2019
Twenty-Four-Hour Ex Vivo Perfusion with Acellular Solution Enables Successful Replantation of Porcine Forelimbs.
Krezdorn N, Macleod F, Tasigiorgos S, Turk M D M, Wo L, Kiwanuka B A H, Lopdrup B I D R, Kollar B, Edelman E, Pomahac B. Twenty-Four-Hour Ex Vivo Perfusion with Acellular Solution Enables Successful Replantation of Porcine Forelimbs. Plastic & Reconstructive Surgery 2019, 144: 608e-618e. PMID: 31568296, DOI: 10.1097/prs.0000000000006084.Peer-Reviewed Original Research
2018
Reduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage
Krezdorn N, Sakthivel D, Turk M, Aycart M, Tasigiorgos S, Bueno E, Sinha I, Pomahac B. Reduced Hypoxia-Related Genes in Porcine Limbs in Ex Vivo Hypothermic Perfusion Versus Cold Storage. Journal Of Surgical Research 2018, 232: 137-145. PMID: 30463709, DOI: 10.1016/j.jss.2018.05.067.Peer-Reviewed Original ResearchConceptsStatic cold storagePerfusion groupIschemia-reperfusion injuryPCR gene arrayPorcine limbsMuscle fiber disruptionIschemic injuryPerfadex solutionPerfused limbMachine perfusionHypothermic perfusionHistological changesPreservation modalityMuscle biopsyLimb replantationPerfusionICE groupLess activationPorcine forelimbDownregulation of genesMolecular changesLimbFiber disruptionExpression levelsReplantation
2017
Acellular Hypothermic Extracorporeal Perfusion Extends Allowable Ischemia Time in a Porcine Whole Limb Replantation Model
Kueckelhaus M, Dermietzel A, Alhefzi M, Aycart M, Fischer S, Krezdorn N, Wo L, Maarouf O, Riella L, Abdi R, Bueno E, Pomahac B. Acellular Hypothermic Extracorporeal Perfusion Extends Allowable Ischemia Time in a Porcine Whole Limb Replantation Model. Plastic & Reconstructive Surgery 2017, 139: 922e-932e. PMID: 28350667, DOI: 10.1097/prs.0000000000003208.Peer-Reviewed Original Research
2016
A Mobile Extracorporeal Extremity Salvage System for Replantation and Transplantation
Kueckelhaus M, Fischer S, Sisk G, Kiwanuka H, Bueno E, Dermietzel A, Alhefzi M, Aycart M, Diehm Y, Pomahac B. A Mobile Extracorporeal Extremity Salvage System for Replantation and Transplantation. Annals Of Plastic Surgery 2016, 76: 355-360. PMID: 26808757, DOI: 10.1097/sap.0000000000000681.Peer-Reviewed Original ResearchConceptsMuscle damageLimb lossPerfusion deviceDeceased organ donorsIschemic muscle damageBilateral hind limbsPreferred treatmentBilateral limbsMuscle biopsyOrgan donorsTraumatic amputationHealthy limbPerfusion solutionReplantationArterial systemTransplantationHypoxic cellsPerfusate compositionHind limbPorcine limbsLimbSignificant differencesYorkshire pigsSalvage systemSimilar changes