2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blotting
2003
Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins
Zhang J, Li W, Sanders MA, Sumpio BE, Asit P, Basson MD. Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins. The FASEB Journal 2003, 17: 1-22. PMID: 12626437, DOI: 10.1096/fj.02-0663fje.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell AdhesionCell DivisionCell LineCollagen Type ICollagen Type IVEnzyme ActivationEpithelial CellsExtracellular Matrix ProteinsFibronectinsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesHumansIntegrin alpha5Integrin alphaVIntestinal MucosaLamininMitogen-Activated Protein KinasesPhosphorylationProtein-Tyrosine KinasesStress, MechanicalConceptsExtracellular signal-regulated protein kinaseMatrix proteinsIntestinal epithelial responsesHuman intestinal epithelial proliferationSignal-regulated protein kinaseJun N-terminal kinaseFocal adhesion kinaseIntestinal epithelial biologyMatrix-dependent mannerN-terminal kinaseExtracellular matrix proteinsEpithelial responseCaco-2 proliferationCaco-2BBe cellsIntestinal epithelial proliferationAdhesion kinaseEpidermal growth factorProtein kinaseERK activationEpithelial biologyEpithelial cell proliferationIntegrin subunitsKinaseAnti-integrin antibodiesMEK blockade
2001
Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells
Li W, Duzgun A, Sumpio B, Basson M. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. AJP Gastrointestinal And Liver Physiology 2001, 280: g75-g87. PMID: 11123200, DOI: 10.1152/ajpgi.2001.280.1.g75.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell DivisionCell MovementCytoskeletal ProteinsEnzyme InhibitorsExtracellular MatrixFlavonoidsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression Regulation, EnzymologicHumansImidazolesIntegrinsIntestinesMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNaphthalenesP38 Mitogen-Activated Protein KinasesPaxillinPhosphoproteinsPhosphorylationProtein-Tyrosine KinasesPyridinesStress, MechanicalTransfectionConceptsFocal adhesion kinaseMAPK extracellular signal-regulated kinaseCaco-2 cellsMitogen-activated protein kinase activationVitro kinase assaysProtein kinase activationSignal-regulated kinaseMitogenic effectProtein kinase C inhibitionBeta1 integrin subunitsCaco-2 proliferationKinase assaysAdhesion kinaseFAK activationERK signalsJNK1 activationKinase activationTerminal kinaseMAPK activationUpstream signalsC-JunFAK inhibitionP38 inhibitionIntegrin subunitsKinaseThe Integrin-Mediated Cyclic Strain-Induced Signaling Pathway in Vascular Endothelial Cells
Frangos S, Knox R, Yano Y, Chen E, Di Luozzo G, Chen A, Sumpio B. The Integrin-Mediated Cyclic Strain-Induced Signaling Pathway in Vascular Endothelial Cells. Endothelium 2001, 8: 1-10. PMID: 11409847, DOI: 10.3109/10623320109063153.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinase (MAPK) familyCytoplasmic protein kinaseProtein kinase familySignal transduction pathwaysFocal adhesion kinaseExtracellular matrix receptorsProcess of phosphorylationEndothelial cellsKinase familySignal transductionTranscription factorsAdhesion kinaseProtein kinaseTransduction pathwaysMatrix receptorsGene expressionTyrosine residuesSignaling pathwaysCyclic circumferential strainVascular endothelial cellsPathwayKinaseIntegrinsHemodynamic forcesVasculature results
2000
Endothelial cell response to different mechanical forces
Azuma N, Duzgun S, Ikeda M, Kito H, Akasaka N, Sasajima T, Sumpio B. Endothelial cell response to different mechanical forces. Journal Of Vascular Surgery 2000, 32: 789-794. PMID: 11013043, DOI: 10.1067/mva.2000.107989.Peer-Reviewed Original ResearchAnimalsAortaCattleCells, CulturedEndothelium, VascularEnzyme ActivationFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesFocal AdhesionsHumansImmunoblottingJNK Mitogen-Activated Protein KinasesMechanoreceptorsMitogen-Activated Protein KinasesPhosphorylationProtein-Tyrosine KinasesSignal TransductionStress, MechanicalEffects of increased ambient pressure on colon cancer cell adhesion
Basson M, Yu C, Herden‐Kirchoff O, Ellermeier M, Sanders M, Merrell R, Sumpio B. Effects of increased ambient pressure on colon cancer cell adhesion. Journal Of Cellular Biochemistry 2000, 78: 47-61. PMID: 10797565, DOI: 10.1002/(sici)1097-4644(20000701)78:1<47::aid-jcb5>3.0.co;2-m.Peer-Reviewed Original ResearchConceptsColon cancer cell adhesionCancer cell adhesionTyrosine phosphorylationCell adhesionPressure-stimulated adhesionFocal adhesion kinaseHuman colon cancer cellsIntestinal epithelial biologyColon cancer cellsColon cancer cell linesHuman colon cancer cell linesBeta1 integrin subunitsHuman colon cancerAdhesion kinaseBovine aortic smooth muscle cellsAortic smooth muscle cellsIntracellular proteinsEpithelial biologyMatrix proteinsAbsence of serumIntegrin subunitsCancer cell linesPrimary cellsSmooth muscle cellsKinase inhibition
1999
Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent
Gahtan V, Wang X, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent. Surgery 1999, 126: 203-207. PMID: 10455885, DOI: 10.1016/s0039-6060(99)70156-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCattleCell Adhesion MoleculesCells, CulturedChemotaxisDose-Response Relationship, DrugFocal Adhesion Protein-Tyrosine KinasesMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularProtein-Tyrosine KinasesThrombospondin 1ConceptsSmooth muscle cell chemotaxisTSP-1-induced chemotaxisThrombospondin-1Protein kinase familyExtracellular signal-regulated proteinExtracellular signal-regulated protein kinase 1/2Vascular smooth muscle cell chemotaxisProtein kinase 1/2Extracellular matrix proteinsCell chemotaxisSerum-free mediumPlatelet-derived growth factorKinase familyKinase 1/2Top chambersFunctional importanceMatrix proteinsERK1/2 activationWestern blot analysisERK1/2 inhibitorBlot analysisERK1/2VSMCPD98059VSMCsThrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells
Gahtan V, Wang X, Ikeda M, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells. Journal Of Vascular Surgery 1999, 29: 1031-1036. PMID: 10359937, DOI: 10.1016/s0741-5214(99)70244-2.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylationAntiphosphotyrosine immunoblottingThrombospondin-1TSP-1-induced VSMC migrationFocal adhesion kinase (FAK) proteinSignal transduction pathwaysFocal adhesion kinaseFocal adhesion plaquesVSMC migrationExtracellular matrix proteinsPlatelet-derived growth factorCrude cell lysatesMolecular weight 68Adhesion kinaseTransduction pathwaysKinase proteinAdhesion plaquesVascular smooth muscle cell proliferationVascular smooth muscle cellsMatrix proteinsCell lysatesPhosphorylationSmooth muscle cell proliferationFAK antibodyProtein bandsExtracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1998
Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals
Han O, Di Li G, Sumpio B, Basson M. Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals. American Journal Of Physiology 1998, 275: g534-g541. PMID: 9724266, DOI: 10.1152/ajpgi.1998.275.3.g534.Peer-Reviewed Original ResearchMechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells
Han O, Sumpio B, Basson M. Mechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells. Biochemical And Biophysical Research Communications 1998, 250: 668-673. PMID: 9784404, DOI: 10.1006/bbrc.1998.9372.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsHumansIntestinesPhosphorylationProtein-Tyrosine KinasesProteinsStress, MechanicalTyrosineConceptsCaco-2 cellsTyrosine kinase activityHuman intestinal Caco-2 cellsIntestinal Caco-2 cellsCaco-2 intestinal cellsRepetitive strainHuman Caco-2 intestinal cellsCaco-2 intestinal epithelial cellsIntestinal epithelial cellsHuman Caco-2 intestinal epithelial cellsKinase activityTyrosine phosphorylationIntestinal cellsEpithelial cellsModulates differentiationParticulate fractionTyrosine phosphorylated proteinsCellsSubcellular distribution
1997
Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrinsGene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1996
Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostinsInvolvement of rho p21 in Cyclic Strain-Induced Tyrosine Phosphorylation of Focal Adhesion Kinase (pp125FAK), Morphological Changes and Migration of Endothelial Cells
Yano Y, Saito Y, Narumiya S, Sumpio B. Involvement of rho p21 in Cyclic Strain-Induced Tyrosine Phosphorylation of Focal Adhesion Kinase (pp125FAK), Morphological Changes and Migration of Endothelial Cells. Biochemical And Biophysical Research Communications 1996, 224: 508-515. PMID: 8702419, DOI: 10.1006/bbrc.1996.1057.Peer-Reviewed Original ResearchAdenosine Diphosphate RiboseADP Ribose TransferasesAnimalsAorta, ThoracicBotulinum ToxinsCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGTP-Binding ProteinsMitomycinsPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesRho GTP-Binding ProteinsStress, Mechanical