2006
Mutational Analysis of RetS, an Unusual Sensor Kinase-Response Regulator Hybrid Required for Pseudomonas aeruginosa Virulence
Laskowski MA, Kazmierczak BI. Mutational Analysis of RetS, an Unusual Sensor Kinase-Response Regulator Hybrid Required for Pseudomonas aeruginosa Virulence. Infection And Immunity 2006, 74: 4462-4473. PMID: 16861632, PMCID: PMC1539586, DOI: 10.1128/iai.00575-06.Peer-Reviewed Original ResearchConceptsType III secretion system proteinsSignal transduction domainsSecretion system proteinsUpregulation of genesPeriplasmic domainSensor kinaseReceiver domainTransmembrane domainRegulator proteinTransduction domainMutational analysisSignaling roleSystem proteinsReciprocal regulationPseudomonas aeruginosaRET activityBiofilm formationVirulence factorsOpportunistic pathogenT3SSProteinRET alleleRETP. aeruginosaKey role
2004
A novel sensor kinase–response regulator hybrid regulates type III secretion and is required for virulence in Pseudomonas aeruginosa
Laskowski MA, Osborn E, Kazmierczak BI. A novel sensor kinase–response regulator hybrid regulates type III secretion and is required for virulence in Pseudomonas aeruginosa. Molecular Microbiology 2004, 54: 1090-1103. PMID: 15522089, PMCID: PMC3650721, DOI: 10.1111/j.1365-2958.2004.04331.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBase SequenceCalciumFemaleGene Expression Regulation, BacterialHistidine KinaseMiceMice, Inbred C57BLProtein KinasesProtein Structure, TertiaryPseudomonas aeruginosaPseudomonas InfectionsRecombinant Fusion ProteinsSignal TransductionTrans-ActivatorsTranscription, GeneticConceptsType III secretion systemTwo-component signaling proteinsCalcium limitationResponse regulator domainType III effectorsBasal transcription rateWild-type parentNorthern blot analysisRegulator domainHistidine kinasePeriplasmic domainTranscriptional activatorEukaryotic cellsTTSS effectorsTranscriptional fusionsTransmembrane domainEnvironmental signalsSignaling proteinsSecretion systemSensor proteinsTTSS genesTranscription rateOperonPseudomonas aeruginosaEffector production
1988
Neuromodulator‐Mediated Phosphorylation of Specific Proteins in a Neurotumor Hybrid Cell Line (NCB‐20)
Berry‐Kravis E, Kazmierczak B, Derechin V, Dawson G. Neuromodulator‐Mediated Phosphorylation of Specific Proteins in a Neurotumor Hybrid Cell Line (NCB‐20). Journal Of Neurochemistry 1988, 50: 1287-1296. PMID: 2450174, DOI: 10.1111/j.1471-4159.1988.tb10606.x.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine2-ChloroadenosineAdenosineAlprostadilAnimalsBrainCalcimycinColforsinCricetinaeCyclic AMPElectrophoresis, Polyacrylamide GelEmbryo, MammalianEnkephalin, LeucineEnkephalin, Leucine-2-AlanineHybrid CellsImmunoassayMiceNerve Tissue ProteinsNeuroblastomaNeurotransmitter AgentsPhosphoproteinsPhosphorylationProtein KinasesSerotoninTumor Cells, CulturedTyrosine 3-MonooxygenaseConceptsCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseCalcium/phospholipid-dependent protein kinasePhospholipid-dependent protein kinaseProtein kinaseHybrid cell linesCalcium/phospholipid-dependent kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinasePC12 cellsStimulation of phosphorylationPhospholipid-dependent kinaseCell linesDifferentiated PC12 cellsMajor synaptic proteinsUnique proteinsSpecific proteinsMajor proteinsKinasePhosphorylation