2022
Pharmacological characterization of 5-iodo-A-85380, a β2-selective nicotinic receptor agonist, in mice
Akinola LS, Bagdas D, Alkhlaif Y, Jackson A, Gurdap CO, Rahimpour E, Carroll FI, Papke RL, Damaj MI. Pharmacological characterization of 5-iodo-A-85380, a β2-selective nicotinic receptor agonist, in mice. Journal Of Psychopharmacology 2022, 36: 1280-1293. PMID: 36321267, PMCID: PMC9817006, DOI: 10.1177/02698811221132214.Peer-Reviewed Original ResearchConceptsNicotinic receptor agonistsReceptor agonistNicotinic acetylcholine receptorsLow sensitivityPharmacological characterizationΑ4β2 receptorsNicotine dependenceNicotinic subunitsPlace preferenceΑ4β2 nAChRsAcetylcholine receptorsPartial agonistFull agonistAgonistsDifferential efficacyBehavioral effectsNeurodegenerative diseasesSubtypesPathological conditionsNAChRsPotential targetPotential roleHypothermiaReceptorsΑ6
2021
Potentiation of (α4)2(β2)3, but not (α4)3(β2)2, nicotinic acetylcholine receptors reduces nicotine self-administration and withdrawal symptoms
Hamouda AK, Bautista MR, Akinola LS, Alkhlaif Y, Jackson A, Carper M, Toma WB, Garai S, Chen YC, Thakur GA, Fowler CD, Damaj MI. Potentiation of (α4)2(β2)3, but not (α4)3(β2)2, nicotinic acetylcholine receptors reduces nicotine self-administration and withdrawal symptoms. Neuropharmacology 2021, 190: 108568. PMID: 33878302, PMCID: PMC8169606, DOI: 10.1016/j.neuropharm.2021.108568.Peer-Reviewed Original ResearchConceptsPositive allosteric modulatorsWithdrawal symptomsHypothermic effectMale miceAntinociceptive effectΑ4β2 nAChRsNicotine withdrawal-induced hyperalgesiaNAChR isoformsNicotine's antinociceptive effectsWithdrawal-induced hyperalgesiaNicotine withdrawal symptomsNicotine addiction treatmentAnxiety-like behaviorNicotinic acetylcholine receptorsDose-dependent mannerNociceptive responsesNicotine withdrawalNicotine intakeSomatic signsNicotine abstinencePharmacological effectsNicotine useAcetylcholine receptorsAffective symptomsPathophysiological processes
2019
The β3 subunit of the nicotinic acetylcholine receptor is required for nicotine withdrawal-induced affective but not physical signs or nicotine reward in mice
Jackson AB, Toma W, Contreras KM, Alkhlaif Y, Damaj MI. The β3 subunit of the nicotinic acetylcholine receptor is required for nicotine withdrawal-induced affective but not physical signs or nicotine reward in mice. Pharmacology Biochemistry And Behavior 2019, 183: 1-5. PMID: 31145916, PMCID: PMC7197262, DOI: 10.1016/j.pbb.2019.05.003.Peer-Reviewed Original ResearchConceptsNicotine withdrawal signsWithdrawal signsNicotine rewardNicotinic acetylcholine receptorsWT miceNicotine dependenceAcetylcholine receptorsNicotine dependence behaviorsNicotine-dependent miceAffective nicotine withdrawal signsΒ3 subunitTobacco use disorderNicotine-induced CPPSimilar somatic symptomsPlace preference testNew molecular targetsAffective withdrawal signsAddictive componentSpontaneous withdrawalWithdrawal studyKO miceFemale miceHealth burdenPhysical signsUse disorders
2018
Knockout of alpha 5 nicotinic acetylcholine receptors subunit alters ethanol-mediated behavioral effects and reward in mice
Dawson A, Wolstenholme JT, Roni MA, Campbell VC, Jackson A, Slater C, Bagdas D, Perez EE, Bettinger JC, De Biasi M, Miles MF, Damaj MI. Knockout of alpha 5 nicotinic acetylcholine receptors subunit alters ethanol-mediated behavioral effects and reward in mice. Neuropharmacology 2018, 138: 341-348. PMID: 29944862, PMCID: PMC6400055, DOI: 10.1016/j.neuropharm.2018.06.031.Peer-Reviewed Original ResearchConceptsΑ5 nAChREthanol consumptionΑ5 nicotinic acetylcholine receptor (nAChR) subunitsAlcohol dependenceBehavioral effectsImportance of nAChRsΑ5 nAChR subunitΑ5-KO miceNicotinic acetylcholine receptor subunitsEthanol-induced hypothermiaAnxiolytic-like responseEthanol drinking behaviorVoluntary ethanol consumptionNicotinic acetylcholine receptorsΑ5 knockout miceAcetylcholine receptor subunitsTwo-bottle choiceDecreases ethanol intakePlace preference assayEthanol-induced behaviorsWild-type controlsKO miceRestraint stressDID paradigmEthanol intakeNew insights on the effects of varenicline on nicotine reward, withdrawal and hyperalgesia in mice
Bagdas D, Alkhlaif Y, Jackson A, Carroll FI, Ditre JW, Damaj MI. New insights on the effects of varenicline on nicotine reward, withdrawal and hyperalgesia in mice. Neuropharmacology 2018, 138: 72-79. PMID: 29860196, PMCID: PMC6054891, DOI: 10.1016/j.neuropharm.2018.05.025.Peer-Reviewed Original ResearchConceptsEffects of vareniclineNicotine withdrawal signsNicotine rewardΑ5 nAChRWithdrawal signsHigh doseKnockout miceΒ2-nAChRsNicotine withdrawal-induced hyperalgesiaAdministration of vareniclineWithdrawal-induced hyperalgesiaΑ7 knockout miceDose-related mannerNicotinic acetylcholine receptorsΑ5 knockout micePlace preference testVarenicline doseCessation treatmentNicotine withdrawalSomatic signsVareniclineΑ7 nAChRsMouse modelCPP testNicotinic subtypes
2017
Reversal of Nicotine Withdrawal Signs Through Positive Allosteric Modulation of α4β2 Nicotinic Acetylcholine Receptors in Male Mice
Hamouda AK, Jackson A, Bagdas D, Damaj M. Reversal of Nicotine Withdrawal Signs Through Positive Allosteric Modulation of α4β2 Nicotinic Acetylcholine Receptors in Male Mice. Nicotine & Tobacco Research 2017, 20: 903-907. PMID: 29059422, PMCID: PMC5991208, DOI: 10.1093/ntr/ntx183.Peer-Reviewed Original ResearchConceptsNicotine withdrawal symptomsSpontaneous nicotine withdrawalNicotine withdrawal signsNicotine withdrawalWithdrawal symptomsPositive allosteric modulatorsCessation aidWithdrawal signsMale miceMouse modelAllosteric modulatorsNicotinic acetylcholine receptor agonistNAChR-positive allosteric modulatorsAcetylcholine receptor agonistDose-dependent reversalICR male miceΑ4β2 nicotinic acetylcholine receptorsPositive allosteric modulationAnxiety-like behaviorNicotinic acetylcholine receptorsPotential clinical useAcute injectionRelapse rateTobacco smokingDay infusionAllosteric modulation of α4β2* nicotinic acetylcholine receptors: Desformylflustrabromine potentiates antiallodynic response of nicotine in a mouse model of neuropathic pain
Bagdas D, Ergun D, Jackson A, Toma W, Schulte M, Damaj M. Allosteric modulation of α4β2* nicotinic acetylcholine receptors: Desformylflustrabromine potentiates antiallodynic response of nicotine in a mouse model of neuropathic pain. European Journal Of Pain 2017, 22: 84-93. PMID: 28809075, PMCID: PMC9829446, DOI: 10.1002/ejp.1092.Peer-Reviewed Original ResearchConceptsPositive allosteric modulatorsChronic neuropathic painNeuropathic painΑ4β2 nAChRsNicotinic acetylcholine receptorsAllosteric modulationAcetylcholine receptorsAntagonist dihydro-β-erythroidineNeuronal nicotinic acetylcholine receptorsChronic constriction injuryEndogenous cholinergic toneNicotine-evoked responsesAnimal pain modelsNicotine-induced antinociceptionDihydro-β-erythroidineMediation of painAlternative treatment strategiesBehavior doseConstriction injuryAntiallodynic effectPain modelPain modulationCholinergic tonePain behaviorAntinociceptive properties
2016
Nicotine Enhances the Hypnotic and Hypothermic Effects of Alcohol in the Mouse
Slater CA, Jackson A, Muldoon PP, Dawson A, O'Brien M, Soll LG, Abdullah R, Carroll FI, Tapper AR, Miles MF, Banks ML, Bettinger JC, Damaj IM. Nicotine Enhances the Hypnotic and Hypothermic Effects of Alcohol in the Mouse. Alcohol Clinical And Experimental Research 2016, 40: 62-72. PMID: 26727524, PMCID: PMC4700556, DOI: 10.1111/acer.12918.Peer-Reviewed Original ResearchMeSH KeywordsAlpha7 Nicotinic Acetylcholine ReceptorAnimalsAzetidinesBody TemperatureCentral Nervous System DepressantsDrug InteractionsEthanolHypnotics and SedativesHypothermiaKetamineMecamylamineMiceMice, Inbred C57BLMice, Inbred DBAMice, KnockoutNicotineNicotinic AgonistsNicotinic AntagonistsPentobarbitalPyridinesReceptors, NicotinicReflex, RightingVareniclineConceptsHypnotic effectsLORR testEtOH intakeReceptor efficacyAcute nicotine injectionDuration of EtOHNicotinic acetylcholine receptor subtypesΑ7 knockout miceNicotinic partial agonist vareniclineAcetylcholine receptor subtypesEffects of nicotineNicotinic antagonist mecamylamineDevelopment of toleranceEffects of EtOHPartial agonist vareniclineAcute injectionAcute nicotineNicotine administrationNicotine exposureAntagonist mecamylamineNicotine injectionHypothermic effectNicotine effectsHypnotic propertiesPharmacological interactions
2015
Effects of Menthol on Nicotine Pharmacokinetic, Pharmacology and Dependence in Mice
Alsharari SD, King JR, Nordman JC, Muldoon PP, Jackson A, Zhu AZ, Tyndale RF, Kabbani N, Damaj MI. Effects of Menthol on Nicotine Pharmacokinetic, Pharmacology and Dependence in Mice. PLOS ONE 2015, 10: e0137070. PMID: 26355604, PMCID: PMC4565647, DOI: 10.1371/journal.pone.0137070.Peer-Reviewed Original ResearchConceptsNicotine pharmacokineticsSubunit expressionBrain regionsAcute pharmacological effectsNicotine-induced antinociceptionNicotine plasma levelsMale ICR miceStriatum of miceEffect of mentholSignificant increaseAddition of mentholAdministration of mentholWestern blot analysisChronic injectionsWithdrawal signsBrain levelsICR miceNicotine cigarettesPlasma levelsSystemic administrationNicotine clearanceNicotinic receptorsPharmacological effectsNicotinic subunitsCommon flavoringIn Vitro and in Vivo Neuronal Nicotinic Receptor Properties of (+)- and (−)-Pyrido[3,4]homotropane [(+)- and (−)-PHT]: (+)-PHT Is a Potent and Selective Full Agonist at α6β2 Containing Neuronal Nicotinic Acetylcholine Receptors
Carroll FI, Navarro H, Mascarella SW, Castro AH, Luetje CW, Wageman CR, Marks MJ, Jackson A, Damaj MI. In Vitro and in Vivo Neuronal Nicotinic Receptor Properties of (+)- and (−)-Pyrido[3,4]homotropane [(+)- and (−)-PHT]: (+)-PHT Is a Potent and Selective Full Agonist at α6β2 Containing Neuronal Nicotinic Acetylcholine Receptors. ACS Chemical Neuroscience 2015, 6: 920-926. PMID: 25891987, PMCID: PMC5589077, DOI: 10.1021/acschemneuro.5b00077.Peer-Reviewed Original ResearchMeSH KeywordsAlpha7 Nicotinic Acetylcholine ReceptorAnimalsConditioning, PsychologicalCorpus StriatumDopamineDose-Response Relationship, DrugMaleMice, Inbred ICRMolecular StructureNeuronsNicotinic AgonistsNicotinic AntagonistsPyridinesRatsReceptors, NicotinicSpatial BehaviorSynaptosomesTropanesXenopus laevisConceptsNicotinic antagonistsNeuronal nicotinic acetylcholine receptorsLow efficacy partial agonistSelective full agonistHot plate testNicotinic acetylcholine receptorsPlace preference studiesNicotine rewardAntinociceptive activityΑ3β4 nAChRsΑ7 nAChRsElectrophysiological studiesΑ4β2 nAChRsAcetylcholine receptorsAgonist activityPartial agonistFull agonistNAChRsFull agonismPartial agonismAntagonistΑ4β2MiceReceptor propertiesHigh potency