1998
Maturation of Human Cyclin E Requires the Function of Eukaryotic Chaperonin CCT
Won K, Schumacher R, Farr G, Horwich A, Reed S. Maturation of Human Cyclin E Requires the Function of Eukaryotic Chaperonin CCT. Molecular And Cellular Biology 1998, 18: 7584-7589. PMID: 9819444, PMCID: PMC109339, DOI: 10.1128/mcb.18.12.7584.Peer-Reviewed Original ResearchConceptsHuman cyclin EChaperonin CCTCyclin EEukaryotic cytosolic chaperonin CCTCytosolic chaperonin CCTEukaryotic chaperonin CCTLarge oligomeric assembliesYeast-based screenG1/S phase transitionCyclin-dependent kinase CDK2ATP-dependent processS phase transitionCCT complexPresence of ATPProteasomal actionCCT functionHuman proteinsKinase CDK2Oligomeric assembliesHuman cellsNative stateCDK2ProteinMaturationBiogenesis
1992
TCP1 complex is a molecular chaperone in tubulin biogenesis
Yaffe M, Farr G, Miklos D, Horwich A, Sternlicht M, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature 1992, 358: 245-248. PMID: 1630491, DOI: 10.1038/358245a0.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCycloheximideDNA-Binding ProteinsIntracellular Signaling Peptides and ProteinsKineticsMacromolecular SubstancesMicrotubule-Associated ProteinsMolecular WeightNuclear ProteinsProtein BiosynthesisProtein ConformationRabbitsReticulocytesRNA, MessengerT-Complex Genome RegionTubulinUbiquitin-Protein LigasesConceptsReticulocyte lysateTubulin subunitsCytosol of eukaryotesComplex polypeptide 1Protease-sensitive conformationRabbit reticulocyte lysateCytosolic chaperonesTubulin biogenesisMajor cytosolic proteinMolecular chaperonesTCP1 complexK proteinCytosolic proteinsΒ heterodimerBiogenesisPolypeptide 1Β-tubulinProteinSubunitsChaperonesMg-ATPK-complexesMolecular targetsNonhydrolysable analogueTubulin