[16] Purification of yeast RNA polymerase II holoenzymes
Koleske AJ, Chao DM, Young RA. [16] Purification of yeast RNA polymerase II holoenzymes. Methods In Enzymology 1996, 273: 176-184. PMID: 8791611, DOI: 10.1016/s0076-6879(96)73018-5.Peer-Reviewed Original ResearchConceptsRNA polymerase II holoenzymeRNA polymerase IIPolymerase IIYeast RNA polymerase II holoenzymeGeneral transcription factors TBPTranscription factors TBPHigh molecular weight complexesMolecular weight complexesTranscriptional regulationTranscription initiationYeast SaccharomycesTranscription studiesHoloenzymeHoloenzyme preparationWeight complexesTranscriptionLow salt bufferExciting cluesLarge formSalt concentrationTFIIBTFIIEAssaysSaccharomycesPurification