2023
Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery
Sundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.Peer-Reviewed Original ResearchConceptsCore protein machineryRelease-ready vesiclesSynaptic vesicle primingVesicle primingProtein machinerySingle-molecule imagingSNAREpin assemblyFunctional intermediatesFunctional reconstitutionMunc13DiacylglycerolCoordinated actionMunc18VesiclesMachineryComplete reconstitutionNew roleSelective effectDetailed characterizationChaperonesRate of caReconstitutionVAMP2ComplexinMutations
2016
Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling
Thakur R, Panda A, Coessens E, Raj N, Yadav S, Balakrishnan S, Zhang Q, Georgiev P, Basak B, Pasricha R, Wakelam M, Ktistakis N, Raghu P. Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. ELife 2016, 5: e18515. PMID: 27848911, PMCID: PMC5125754, DOI: 10.7554/elife.18515.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation Factor 1AnimalsCell MembraneCytoplasmic VesiclesDrosophila melanogasterDrosophila ProteinsEndocytosisGene ExpressionGenetic Complementation TestGuanosine TriphosphateLightPhosphatidic AcidsPhospholipase DPhotic StimulationPhotoreceptor Cells, InvertebrateRab GTP-Binding ProteinsRab7 GTP-Binding ProteinsRhodopsinVision, OcularConceptsPlasma membranePLD activityRetromer-dependent recyclingWild-type photoreceptorsMembrane endocytosisDependent recyclingPhospholipase D activityPhosphatidic acidRhabdomere sizeRhodopsin proteinEndocytosisCell bodiesPhotoreceptorsReduced levelsActivity couplesD activityComplex functionsMembraneTurnoverRab7ProteinVesiclesPhenotypeConsequent changesActivity