2022
Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation
Landajuela A, Braun M, Martínez-Calvo A, Rodrigues CDA, Gomis Perez C, Doan T, Rudner DZ, Wingreen NS, Karatekin E. Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation. Current Biology 2022, 32: 4186-4200.e8. PMID: 36041438, PMCID: PMC9730832, DOI: 10.1016/j.cub.2022.08.014.Peer-Reviewed Original ResearchConceptsMother cell membraneMembrane fissionMother cellsDNA translocationMembrane tensionLarger mother cellMother cell cytoplasmCell membraneHigh membrane tensionSmaller foresporeDNA translocaseEnergy-limited conditionsForespore compartmentAsymmetric divisionEndospore formationMembrane compartmentsMembrane necksSpore developmentCell divisionMolecular basisForesporeMembrane flowBacillus subtilisCell cytoplasmFisB
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyes