2023
Tracking antimalarial drug resistance using mosquito blood meals: a cross-sectional study
Ehrlich H, Somé A, Bazié T, Ebou C, Dembélé E, Balma R, Goodwin J, Wade M, Bei A, Ouédraogo J, Foy B, Dabiré R, Parikh S. Tracking antimalarial drug resistance using mosquito blood meals: a cross-sectional study. The Lancet Microbe 2023, 4: e461-e469. PMID: 37086737, PMCID: PMC10365133, DOI: 10.1016/s2666-5247(23)00063-0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimalarialsCross-Sectional StudiesCulicidaeFolic Acid AntagonistsHumansPlasmodium falciparumPolymerase Chain ReactionConceptsMosquito blood mealsAntimalarial drug resistanceSurvey 3Blood-fed mosquitoesBlood samplesSurvey 1Survey 2Blood mealDrug resistanceUltrasensitive quantitative PCRHuman blood samplesCross-sectional studyMargin of equivalenceStrong surveillance systemCross-sectional surveySupplementary Materials sectionMarker of clonalityPragmatic thresholdAntimalarial resistanceDrug susceptibilityInfectious diseasesPlasmodium falciparumNational InstituteTolerabilityMaterial section
2017
High resolution melting: a useful field-deployable method to measure dhfr and dhps drug resistance in both highly and lowly endemic Plasmodium populations
Ndiaye YD, Diédhiou CK, Bei AK, Dieye B, Mbaye A, Mze NP, Daniels RF, Ndiaye IM, Déme AB, Gaye A, Sy M, Ndiaye T, Badiane AS, Ndiaye M, Premji Z, Wirth DF, Mboup S, Krogstad D, Volkman SK, Ahouidi AD, Ndiaye D. High resolution melting: a useful field-deployable method to measure dhfr and dhps drug resistance in both highly and lowly endemic Plasmodium populations. Malaria Journal 2017, 16: 153. PMID: 28420422, PMCID: PMC5395743, DOI: 10.1186/s12936-017-1811-2.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentChildChild, PreschoolDihydropteroate SynthaseDrug ResistanceGenotypeGenotyping TechniquesHumansMalaria, FalciparumMolecular Diagnostic TechniquesPlasmodiumPoint-of-Care SystemsPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSenegalTanzaniaTetrahydrofolate DehydrogenaseTransition TemperatureYoung AdultConceptsMalaria endemic sitesDrug resistanceHigh prevalenceUncomplicated Plasmodium falciparum malariaPolymerase chain reaction-restriction fragment length polymorphism methodologyAnti-malarial drug usePlasmodium falciparum malariaEndemic sitesEmergence of resistanceMultiplicity of infectionFalciparum malariaFragment length polymorphism methodologyDHFR mutationsBlood samplesMalaria endemicityPCR-RFLP genotypingPCR/RFLPTreatment policyCodon 540Drug usePlasmodium populationsMixed allelesEarly detectionCodons 51Mixed infections
2014
Analysis of pfhrp2 genetic diversity in Senegal and implications for use of rapid diagnostic tests
Deme AB, Park DJ, Bei AK, Sarr O, Badiane AS, Gueye Pel H, Ahouidi A, Ndir O, Mboup S, Wirth DF, Ndiaye D, Volkman SK. Analysis of pfhrp2 genetic diversity in Senegal and implications for use of rapid diagnostic tests. Malaria Journal 2014, 13: 34. PMID: 24472178, PMCID: PMC3913323, DOI: 10.1186/1475-2875-13-34.Peer-Reviewed Original ResearchConceptsGenetic diversityNucleotide diversityNon-synonymous nucleotide diversityRepeat length polymorphismAmino acid repeatsPfhrp2 geneLength polymorphismExtent of polymorphismEssential genesParasite ecologyAcid repeatsDiversity surveysParasite proteinsBp regionGenesProtein 2Polymorphic genesRepeatsDiversityHistidine-rich protein 2PfHRP2 antigenSenegalese National Malaria Control ProgrammeHRP2 detectionAntigen diversityPlasmodium falciparum