2017
Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities
Didychuk A, Montemayor E, Carrocci T, DeLaitsch A, Lucarelli S, Westler W, Brow D, Hoskins A, Butcher S. Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities. Nature Communications 2017, 8: 497. PMID: 28887445, PMCID: PMC5591277, DOI: 10.1038/s41467-017-00484-w.Peer-Reviewed Original ResearchMeSH KeywordsCatalytic DomainCrystallography, X-RayEvolution, MolecularGenetic VariationHumansModels, MolecularPhosphoric Diester HydrolasesProtein BindingProtein DomainsRibonucleoprotein, U4-U6 Small NuclearRNA, Small NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSubstrate SpecificityConceptsU6 snRNP assemblySmall nuclear ribonucleoproteinSnRNP assemblyCognate RNA-binding proteinsTerminal 3'-phosphateU6 small nuclear ribonucleoproteinsRNA-binding proteinsAnti-cooperative interactionsCyclic phosphodiesterase activitySpliceosome assemblyU6 RNAHuman orthologNuclear ribonucleoproteinUSB1SpliceosomeYeastProteinPhosphodiesterase activityAntagonist proteinComplex seriesAssemblyPrp24LHP1OrthologsSnRNA
2012
Structure of Pisum sativum Rubisco with bound ribulose 1,5‐bisphosphate
Loewen P, Didychuk A, Switala J, Perez-Luque R, Fita I, Loewen M. Structure of Pisum sativum Rubisco with bound ribulose 1,5‐bisphosphate. Acta Crystallographica Section F: Structural Biology Communications 2012, 69: 10-14. PMID: 23295478, PMCID: PMC3539695, DOI: 10.1107/s1744309112047549.Peer-Reviewed Original ResearchConceptsRibulose 1,5-bisphosphateRibulose-1,5-bisphosphate carboxylase/oxygenaseSubstrate ribulose 1,5-bisphosphateHanging-drop vapor diffusionSmall subunit sequenceRibulose-1,5-bisphosphateDiffraction-quality crystalsActive-site conformationActive siteRubisco structureRubiscoClosed conformationHexadecameric complexPisum sativumA-resolutionQuaternary structureGarden peaVapor diffusionX-ray diffraction dataPulse cropsCarboxylase/oxygenaseLys201ConformationSitesDiffraction data