2018
The life of U6 small nuclear RNA, from cradle to grave
Didychuk A, Butcher S, Brow D. The life of U6 small nuclear RNA, from cradle to grave. RNA 2018, 24: 437-460. PMID: 29367453, PMCID: PMC5855946, DOI: 10.1261/rna.065136.117.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNANuclear RNAProcess of RNA splicingCatalyzes intron removalEukaryotic gene expressionPre-mRNA substrateUridine-rich small nuclear RNAsRemoval of intronsPrecursor messenger RNACryo-EM structureSplicing cycleNoncoding transcriptsCatalytic coreProtein partnersRNA splicingIntron removalSplice siteGenetic dataMacromolecular machinesSpliceosomeGene expressionSplicingConformational changes
2015
Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA
Cornilescu G, Didychuk A, Rodgers M, Michael L, Burke J, Montemayor E, Hoskins A, Butcher S. Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal Of Molecular Biology 2015, 428: 777-789. PMID: 26655855, PMCID: PMC4790120, DOI: 10.1016/j.jmb.2015.11.026.Peer-Reviewed Original ResearchConceptsU4/U6 di-snRNAScattering dataTri-snRNPAnalysis of RNA structureCryo-electron microscopy structureResonance energy transfer dataX-ray scattering dataMagnetic susceptibility anisotropyRNA structureAssembled spliceosomeRibonucleoprotein complexTertiary interactionsU4/U6Energy transfer dataAlignment mediaSusceptibility anisotropyCryo-electronAlignment tensorStructural investigationsRNAX-rayNucleic acidsStructural analysisWAXS dataY-shaped structure