Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA
Cornilescu G, Didychuk A, Rodgers M, Michael L, Burke J, Montemayor E, Hoskins A, Butcher S. Structural Analysis of Multi-Helical RNAs by NMR–SAXS/WAXS: Application to the U4/U6 di-snRNA. Journal Of Molecular Biology 2015, 428: 777-789. PMID: 26655855, PMCID: PMC4790120, DOI: 10.1016/j.jmb.2015.11.026.Peer-Reviewed Original ResearchConceptsU4/U6 di-snRNAScattering dataTri-snRNPAnalysis of RNA structureCryo-electron microscopy structureResonance energy transfer dataX-ray scattering dataMagnetic susceptibility anisotropyRNA structureAssembled spliceosomeRibonucleoprotein complexTertiary interactionsU4/U6Energy transfer dataAlignment mediaSusceptibility anisotropyCryo-electronAlignment tensorStructural investigationsRNAX-rayNucleic acidsStructural analysisWAXS dataY-shaped structure