2018
The life of U6 small nuclear RNA, from cradle to grave
Didychuk A, Butcher S, Brow D. The life of U6 small nuclear RNA, from cradle to grave. RNA 2018, 24: 437-460. PMID: 29367453, PMCID: PMC5855946, DOI: 10.1261/rna.065136.117.Peer-Reviewed Original ResearchConceptsU6 small nuclear RNASmall nuclear RNAPre-mRNANuclear RNAProcess of RNA splicingCatalyzes intron removalEukaryotic gene expressionPre-mRNA substrateUridine-rich small nuclear RNAsRemoval of intronsPrecursor messenger RNACryo-EM structureSplicing cycleNoncoding transcriptsCatalytic coreProtein partnersRNA splicingIntron removalSplice siteGenetic dataMacromolecular machinesSpliceosomeGene expressionSplicingConformational changes
2017
Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core
Montemayor E, Didychuk A, Liao H, Hu P, Brow D, Butcher S. Structure and conformational plasticity of the U6 small nuclear ribonucleoprotein core. Acta Crystallographica Section D, Structural Biology 2017, 73: 1-8. PMID: 28045380, PMCID: PMC5331471, DOI: 10.1107/s2059798316018222.Peer-Reviewed Original ResearchInternal stem-loopRNA recognition motifSmall nuclear ribonucleoproteinU6 internal stem-loopSplicing of precursor messenger RNAU6 small nuclear RNAProtein-RNA interfacesU6 small nuclear ribonucleoproteinsPrecursor messenger RNASmall nuclear RNAGel shift assaysStem-loopRibonucleoprotein complexCrystal structureGenetic dataNuclear ribonucleoproteinNuclear RNAConformational plasticityWild-typePrp24RNASpliceosomeElectrophoretic mobilityMessenger RNARibonucleoprotein