1999
A Human Anti-HIV Autoantibody Enhances EBV Transformation and HIV Infection
Cavacini L, Wisnewski A, Peterson J, Montefiori D, Emes C, Duval M, Kingsbury G, Wang A, Scadden D, Posner M. A Human Anti-HIV Autoantibody Enhances EBV Transformation and HIV Infection. Clinical Immunology 1999, 93: 263-273. PMID: 10600338, DOI: 10.1006/clim.1999.4790.Peer-Reviewed Original ResearchConceptsUninfected lymphoid cellsLymphoid cellsEBV transformationB cellsHIV-1-infected cellsHIV-1 infectionComplement-dependent mannerCross-reactive natureB cell proliferationNormal B cellsHIV infectionHIV pathogenesisNK cellsAntibody responseFree gp120Immunoglobulin productionT cellsHIV-1Human mAbsViral infectionVH3 family genesIgG mAbsHuman IgG mAbsUninfected cellsInfection
1998
Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41
Cavacini L, Emes C, Wisnewski A, Power J, Lewis G, Montefiori D, Posner M. Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41. AIDS Research And Human Retroviruses 1998, 14: 1271-1280. PMID: 9764911, DOI: 10.1089/aid.1998.14.1271.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, MonoclonalBlotting, WesternCloning, MolecularComplement System ProteinsGenes, ImmunoglobulinHIV Envelope Protein gp120HIV Envelope Protein gp41HIV InfectionsHIV-1HumansImmunodominant EpitopesImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMiceMolecular Sequence DataSequence Analysis, DNAConceptsHuman monoclonal antibodyHuman Monoclonal Antibody ReactiveHIV-1-infected individualsImmunodominant regionCD4-binding siteAntigen-driven responseComplement-dependent mannerHIV Type 1 Glycoprotein 41Presence of complementMonoclonal Antibodies ReactiveLaboratory isolatesSoluble CD4Nonprotective antibodiesPrecursor gp160Antibody reactiveAntigen specificityViral infectionGp41 regionExtensive somatic mutationCytometric analysisAntibody clonotypesMonoclonal antibodiesPepscan analysisUninfected cellsFunctional activityPhase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120
Cavacini LA, Samore MH, Gambertoglio J, Jackson B, Duval M, Wisnewski A, Hammer S, Koziel C, Trapnell C, Posner MR. Phase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120. AIDS Research And Human Retroviruses 1998, 14: 545-550. PMID: 9591708, DOI: 10.1089/aid.1998.14.545.Peer-Reviewed Original ResearchConceptsSingle doseHIV Type 1 Glycoprotein 120HIV-1-infected individualsHIV-1-infected cellsAnti-HIV-1 activityFunctional activityCD4-binding siteCD4 binding siteHuman monoclonal antibodyAbility of serumInfused antibodyQuantitative microcultureAntibody infusionEscalation studyFull functional activityGlycoprotein 120Primary isolatesHIV-1Immune responseSingle injectionGp120-CD4PatientsSite antibodiesCD4Spectrum of isolates
1996
Human antibody variable region gene usage in HIV-1 infection.
Wisnewski A, Cavacini L, Posner M. Human antibody variable region gene usage in HIV-1 infection. JAIDS Journal Of Acquired Immune Deficiency Syndromes 1996, 11: 31-8. PMID: 8528730, DOI: 10.1097/00042560-199601010-00004.Peer-Reviewed Original ResearchConceptsHIV-1 infectionHIV-1 pathogenesisVariable region gene usageRegion gene usageGene usageHuman immunodeficiency virus type 1 (HIV-1) infectionHIV-1-infected patientsVirus type 1 infectionHuman humoral immune responseAnti-HIV-1 antibodiesDifferent lymphoid compartmentsType 1 infectionHumoral immune responsePeripheral blood lymphocytesPolymerase chain reaction studiesHuman monoclonal antibodyAIDS patientsInfected patientsBlood lymphocytesLymphoid compartmentsGene expressionHIV-1Immune responseSerum IgSerological studies