1998
Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41
Cavacini L, Emes C, Wisnewski A, Power J, Lewis G, Montefiori D, Posner M. Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41. AIDS Research And Human Retroviruses 1998, 14: 1271-1280. PMID: 9764911, DOI: 10.1089/aid.1998.14.1271.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, MonoclonalBlotting, WesternCloning, MolecularComplement System ProteinsGenes, ImmunoglobulinHIV Envelope Protein gp120HIV Envelope Protein gp41HIV InfectionsHIV-1HumansImmunodominant EpitopesImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMiceMolecular Sequence DataSequence Analysis, DNAConceptsHuman monoclonal antibodyHuman Monoclonal Antibody ReactiveHIV-1-infected individualsImmunodominant regionCD4-binding siteAntigen-driven responseComplement-dependent mannerHIV Type 1 Glycoprotein 41Presence of complementMonoclonal Antibodies ReactiveLaboratory isolatesSoluble CD4Nonprotective antibodiesPrecursor gp160Antibody reactiveAntigen specificityViral infectionGp41 regionExtensive somatic mutationCytometric analysisAntibody clonotypesMonoclonal antibodiesPepscan analysisUninfected cellsFunctional activityPhase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120
Cavacini LA, Samore MH, Gambertoglio J, Jackson B, Duval M, Wisnewski A, Hammer S, Koziel C, Trapnell C, Posner MR. Phase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120. AIDS Research And Human Retroviruses 1998, 14: 545-550. PMID: 9591708, DOI: 10.1089/aid.1998.14.545.Peer-Reviewed Original ResearchConceptsSingle doseHIV Type 1 Glycoprotein 120HIV-1-infected individualsHIV-1-infected cellsAnti-HIV-1 activityFunctional activityCD4-binding siteCD4 binding siteHuman monoclonal antibodyAbility of serumInfused antibodyQuantitative microcultureAntibody infusionEscalation studyFull functional activityGlycoprotein 120Primary isolatesHIV-1Immune responseSingle injectionGp120-CD4PatientsSite antibodiesCD4Spectrum of isolates
1997
In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies
Elliot BC, Wisnewski AV, Johnson J, Fenwick-Smith D, Wiest P, Hamer D, Kresina T, Flanigan TP. In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies. Infection And Immunity 1997, 65: 3933-3935. PMID: 9284173, PMCID: PMC175560, DOI: 10.1128/iai.65.9.3933-3935.1997.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyC. parvum infectionParvum infectionEnzyme-linked immunosorbent assayMonoclonal antibodiesHuman immunodeficiency virus-seronegative womenCell linesHuman enterocyte cell lineHigher specific antibody titersCryptosporidium parvum infectionAnti-Cryptosporidium antibodiesPeripheral blood lymphocytesSpecific antibody titersMonoclonal cell linesEnterocyte cell lineAntibody-secreting clonesC. parvum sporozoitesImmunoglobulin therapyAntibody titersBlood lymphocytesFatal illnessPotential therapyHypo-osmolar electrofusionTumor cell linesInfection
1996
Human antibody variable region gene usage in HIV-1 infection.
Wisnewski A, Cavacini L, Posner M. Human antibody variable region gene usage in HIV-1 infection. JAIDS Journal Of Acquired Immune Deficiency Syndromes 1996, 11: 31-8. PMID: 8528730, DOI: 10.1097/00042560-199601010-00004.Peer-Reviewed Original ResearchConceptsHIV-1 infectionHIV-1 pathogenesisVariable region gene usageRegion gene usageGene usageHuman immunodeficiency virus type 1 (HIV-1) infectionHIV-1-infected patientsVirus type 1 infectionHuman humoral immune responseAnti-HIV-1 antibodiesDifferent lymphoid compartmentsType 1 infectionHumoral immune responsePeripheral blood lymphocytesPolymerase chain reaction studiesHuman monoclonal antibodyAIDS patientsInfected patientsBlood lymphocytesLymphoid compartmentsGene expressionHIV-1Immune responseSerum IgSerological studies
1994
Human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in Schistosoma japonicum infection.
Wisnewski AV, Olds GR, Kresina TF. Human monoclonal antibodies heterogeneously express a human cross-reactive idiotype associated with immune function in Schistosoma japonicum infection. Human Antibodies 1994, 5: 178-82. PMID: 7756581.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyEpstein-Barr virusSchistosoma japonicum infectionCross-reactive idiotypeJaponicum infectionImmune functionMonoclonal antibodiesSoluble egg antigenS. japonicum infectionWestern blot profilesSchistosomiasis patientsBarr virusEgg antigenHuman idiotypeIdiotype expressionReactive idiotypeMultiple antigensSerological analysisS. japonicumIdiotypeInfectionAntigenHeterogeneous expressionLevel of suppressionPatients
1992
Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA
1991
An immunoregulatory human monoclonal antibody in schistosomiasis japonica
Kresina T, Guan X, Posner M, Wisnewski A, Olds G. An immunoregulatory human monoclonal antibody in schistosomiasis japonica. Human Antibodies 1991, 2: 42-45. PMID: 1651785, DOI: 10.3233/hab-1991-2107.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesEgg antigenAntigen-induced blastogenesisAdult wormsEpstein-Barr virusSoluble egg antigenOS cell linesWestern blot analysisChronic schistosomiasisSoluble eggBarr virusChinese patientsChronic diseasesAntibody-producing hybridomasAntigen reactivitySchistosomiasis japonicaCarbohydrate antigensWestern blotDecompression operationMurine splenocytesSplenocytesAntibodiesAntigenSchistosoma japonicum