Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41
Cavacini L, Emes C, Wisnewski A, Power J, Lewis G, Montefiori D, Posner M. Functional and Molecular Characterization of Human Monoclonal Antibody Reactive with the Immunodominant Region of HIV Type 1 Glycoprotein 41. AIDS Research And Human Retroviruses 1998, 14: 1271-1280. PMID: 9764911, DOI: 10.1089/aid.1998.14.1271.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, MonoclonalBlotting, WesternCloning, MolecularComplement System ProteinsGenes, ImmunoglobulinHIV Envelope Protein gp120HIV Envelope Protein gp41HIV InfectionsHIV-1HumansImmunodominant EpitopesImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMiceMolecular Sequence DataSequence Analysis, DNAConceptsHuman monoclonal antibodyHuman Monoclonal Antibody ReactiveHIV-1-infected individualsImmunodominant regionCD4-binding siteAntigen-driven responseComplement-dependent mannerHIV Type 1 Glycoprotein 41Presence of complementMonoclonal Antibodies ReactiveLaboratory isolatesSoluble CD4Nonprotective antibodiesPrecursor gp160Antibody reactiveAntigen specificityViral infectionGp41 regionExtensive somatic mutationCytometric analysisAntibody clonotypesMonoclonal antibodiesPepscan analysisUninfected cellsFunctional activityPhase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120
Cavacini LA, Samore MH, Gambertoglio J, Jackson B, Duval M, Wisnewski A, Hammer S, Koziel C, Trapnell C, Posner MR. Phase I Study of a Human Monoclonal Antibody Directed against the CD4-Binding Site of HIV Type 1 Glycoprotein 120. AIDS Research And Human Retroviruses 1998, 14: 545-550. PMID: 9591708, DOI: 10.1089/aid.1998.14.545.Peer-Reviewed Original ResearchConceptsSingle doseHIV Type 1 Glycoprotein 120HIV-1-infected individualsHIV-1-infected cellsAnti-HIV-1 activityFunctional activityCD4-binding siteCD4 binding siteHuman monoclonal antibodyAbility of serumInfused antibodyQuantitative microcultureAntibody infusionEscalation studyFull functional activityGlycoprotein 120Primary isolatesHIV-1Immune responseSingle injectionGp120-CD4PatientsSite antibodiesCD4Spectrum of isolates