2017
Genomic insights into the ancient spread of Lyme disease across North America
Walter KS, Carpi G, Caccone A, Diuk-Wasser MA. Genomic insights into the ancient spread of Lyme disease across North America. Nature Ecology & Evolution 2017, 1: 1569-1576. PMID: 29185509, PMCID: PMC6431794, DOI: 10.1038/s41559-017-0282-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArachnid VectorsBorrelia burgdorferiCanadaEvolution, MolecularGenetic VariationGenome, BacterialIxodesLyme DiseaseUnited StatesConceptsHistory of spreadEvolutionary historyNorth AmericaLyme disease epidemicLast Glacial MaximumAncient spreadLyme disease bacteriaComplex evolutionary historyPast centuryGlacial MaximumHistoryGenomic insightsEvolutionary changeAmericaUndocumented levelB. burgdorferi s.Large collectionSensu strictoBurgdorferi s.Vector-borne diseasesSouthern CanadaLand use changeBorrelia burgdorferi sensu strictoCenturyDisease bacteria
2016
Babesia microti from humans and ticks hold a genomic signature of strong population structure in the United States
Carpi G, Walter KS, Mamoun CB, Krause PJ, Kitchen A, Lepore TJ, Dwivedi A, Cornillot E, Caccone A, Diuk-Wasser MA. Babesia microti from humans and ticks hold a genomic signature of strong population structure in the United States. BMC Genomics 2016, 17: 888. PMID: 27821055, PMCID: PMC5100190, DOI: 10.1186/s12864-016-3225-x.Peer-Reviewed Original ResearchConceptsStrong population structurePopulation structureGenome-wide diversityDifferentiated genetic clustersRecent population expansionB. microti samplesTick-borne apicomplexan parasiteB. microtiNortheastern USAGene flowEvolutionary originApicomplexan parasitesApicoplast genomeGenetic clustersGenomic variationPopulation expansionGeographic rangeRange expansionGenomic signaturesCurrent diversityHuman-derived samplesInfectious phenotypeTick vectorCapture strategyDiversityVectors as Epidemiological Sentinels: Patterns of Within-Tick Borrelia burgdorferi Diversity
Walter KS, Carpi G, Evans BR, Caccone A, Diuk-Wasser MA. Vectors as Epidemiological Sentinels: Patterns of Within-Tick Borrelia burgdorferi Diversity. PLOS Pathogens 2016, 12: e1005759. PMID: 27414806, PMCID: PMC4944968, DOI: 10.1371/journal.ppat.1005759.Peer-Reviewed Original ResearchConceptsPathogen diversityPositive selectionVertebrate hostsHost pathogen diversityLyme disease bacteriaNatural transmission cycleNatural populationsPathogen evolutionGenomic variationHost diversityMajority of ticksHost processesDiverse inoculumUndocumented levelEvolutionary processesTick diversityDisease vectorsMixed strain infectionsImmune complementIndividual ticksDiversityEpidemiological sentinelsTick vectorTransmission cycleHost
2015
Whole genome capture of vector-borne pathogens from mixed DNA samples: a case study of Borrelia burgdorferi
Carpi G, Walter KS, Bent SJ, Hoen AG, Diuk-Wasser M, Caccone A. Whole genome capture of vector-borne pathogens from mixed DNA samples: a case study of Borrelia burgdorferi. BMC Genomics 2015, 16: 434. PMID: 26048573, PMCID: PMC4458057, DOI: 10.1186/s12864-015-1634-x.Peer-Reviewed Original ResearchConceptsWhole-genome captureGenome captureGenomic studiesPopulation genomic studiesArthropod vectorsPathogen DNABorrelia burgdorferi genomeNext-generation sequencing technologiesWhole genome sequencesMixed DNA templatesCulture-independent methodsGeneration sequencing technologyPathogen sequence dataFine-resolution studiesGenome sequenceVector-borne pathogensMixed DNA samplesSingle nucleotide polymorphismsComplete genomeEvolutionary dynamicsSequence dataSequencing technologiesDisease vectorsStudy systemDNA template