2020
Phylogeography and population structure of the tsetse fly Glossina pallidipes in Kenya and the Serengeti ecosystem
Bateta R, Saarman NP, Okeyo WA, Dion K, Johnson T, Mireji PO, Okoth S, Malele I, Murilla G, Aksoy S, Caccone A. Phylogeography and population structure of the tsetse fly Glossina pallidipes in Kenya and the Serengeti ecosystem. PLOS Neglected Tropical Diseases 2020, 14: e0007855. PMID: 32092056, PMCID: PMC7058365, DOI: 10.1371/journal.pntd.0007855.Peer-Reviewed Original Research
2019
Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda
Schneider DI, Saarman N, Onyango MG, Hyseni C, Opiro R, Echodu R, O’Neill M, Bloch D, Vigneron A, Johnson TJ, Dion K, Weiss BL, Opiyo E, Caccone A, Aksoy S. Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda. PLOS Neglected Tropical Diseases 2019, 13: e0007340. PMID: 31369548, PMCID: PMC6692048, DOI: 10.1371/journal.pntd.0007340.Peer-Reviewed Original ResearchThe population genomics of multiple tsetse fly (Glossina fuscipes fuscipes) admixture zones in Uganda
Saarman NP, Opiro R, Hyseni C, Echodu R, Opiyo EA, Dion K, Johnson T, Aksoy S, Caccone A. The population genomics of multiple tsetse fly (Glossina fuscipes fuscipes) admixture zones in Uganda. Molecular Ecology 2019, 28: 66-85. PMID: 30471158, PMCID: PMC9642080, DOI: 10.1111/mec.14957.Peer-Reviewed Original ResearchConceptsAdmixture zonePopulation genomicsHybrid zoneMitochondrial cytochrome oxidase IGenome-wide admixtureProcess of speciationCytochrome oxidase IPatterns of divergenceMaternal inheritance patternDivergent lineagesReproductive barriersSecondary contactOxidase IEvolutionary biologyApproximate Bayesian computationGenetic lineagesParental typesGenetic markersInheritance patternGenomicsLineagesMultiple generationsBayesian computationSpeciationRiver network
2018
Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya.
Okeyo WA, Saarman NP, Bateta R, Dion K, Mengual M, Mireji PO, Ouma C, Okoth S, Murilla G, Aksoy S, Caccone A. Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya. American Journal Of Tropical Medicine And Hygiene 2018, 99: 945-953. PMID: 30105964, PMCID: PMC6159567, DOI: 10.4269/ajtmh.18-0154.Peer-Reviewed Original ResearchConceptsGenetic differentiationPopulation dynamicsDispersal patternsTsetse fliesLow genetic diversityGreater genetic differentiationEvidence of admixtureWestern clusterEastern clusterRecent anthropogenic influencesAnimal African trypanosomiasisGenetic connectivityWestern relativesGenetic diversityMicrosatellite lociPopulation structureGenotypic dataWidespread signalDisease vectorsMajor vectorVector control effortsGreat Rift ValleyDemographic parametersFliesVector control programsUncovering Genomic Regions Associated with Trypanosoma Infections in Wild Populations of the Tsetse Fly Glossina fuscipes
Gloria-Soria A, Dunn WA, Yu X, Vigneron A, Lee KY, Li M, Weiss BL, Zhao H, Aksoy S, Caccone A. Uncovering Genomic Regions Associated with Trypanosoma Infections in Wild Populations of the Tsetse Fly Glossina fuscipes. G3: Genes, Genomes, Genetics 2018, 8: 887-897. PMID: 29343494, PMCID: PMC5844309, DOI: 10.1534/g3.117.300493.Peer-Reviewed Original ResearchConceptsGenomic resourcesRelated speciesCandidate genesNonmodel organismsTranscript librariesFunctional genomicsTranscriptome assemblyFunctional annotationWild populationsPhysiological traitsGenomic regionsDNA regulationMost speciesRNAseq experimentsGenetic basisLaboratory coloniesGenesFunctional studiesPolymorphic sitesSpeciesTrypanosome infection statusGenomeNew assemblyTrypanosoma infectionFlies
2017
Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
Okeyo WA, Saarman NP, Mengual M, Dion K, Bateta R, Mireji PO, Okoth S, Ouma JO, Ouma C, Ochieng J, Murilla G, Aksoy S, Caccone A. Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya. Parasites & Vectors 2017, 10: 471. PMID: 29017572, PMCID: PMC5635580, DOI: 10.1186/s13071-017-2415-y.Peer-Reviewed Original ResearchConceptsTemporal genetic differentiationGenetic diversityGenetic differentiationGenetic bottleneckGenetic variationNational ParkTemporal genetic variationExtensive control measuresAnimal African trypanosomiasisAllelic richnessPairwise FSTGenetic driftNe estimatesHabitat alterationPopulation geneticsTemporal differentiationMicrosatellite markersRuma National ParkClosest sampling sitesG. pallidipesMajor vectorTsetse fliesVector populationsDifferentiationDiversityMultiple evolutionary origins of Trypanosoma evansi in Kenya
Kamidi CM, Saarman NP, Dion K, Mireji PO, Ouma C, Murilla G, Aksoy S, Schnaufer A, Caccone A. Multiple evolutionary origins of Trypanosoma evansi in Kenya. PLOS Neglected Tropical Diseases 2017, 11: e0005895. PMID: 28880965, PMCID: PMC5605091, DOI: 10.1371/journal.pntd.0005895.Peer-Reviewed Original ResearchConceptsT. brucei strainsDifferent genetic backgroundsEvolutionary originGenetic diversityBrucei strainsMultiple evolutionary originsGenetic backgroundTsetse fly vectorT. evansiLethal human diseaseIndependent originsPolymorphic microsatellitesT. bruceiEvansi isolatesGenetic unitsHuman diseasesObligate linkBruceiEvansi strainsT. brucei bruceiTsetse fliesFly vectorsFliesDiversityBrucei bruceiGenetic diversity of Glossina fuscipes fuscipes along the shores of Lake Victoria in Tanzania and Kenya: implications for management
Manangwa O, Nkwengulila G, Ouma JO, Mramba F, Malele I, Dion K, Sistrom M, Khan F, Aksoy S, Caccone A. Genetic diversity of Glossina fuscipes fuscipes along the shores of Lake Victoria in Tanzania and Kenya: implications for management. Parasites & Vectors 2017, 10: 268. PMID: 28558831, PMCID: PMC5450392, DOI: 10.1186/s13071-017-2201-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGenetic VariationInsect VectorsKenyaLakesMicrosatellite RepeatsTanzaniaTsetse FliesGenetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control
Opiro R, Saarman NP, Echodu R, Opiyo EA, Dion K, Halyard A, Dunn AW, Aksoy S, Caccone A. Genetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control. PLOS Neglected Tropical Diseases 2017, 11: e0005485. PMID: 28453513, PMCID: PMC5425221, DOI: 10.1371/journal.pntd.0005485.Peer-Reviewed Original ResearchConceptsAnimal African trypanosomiasisGenetic clustersEffective population size estimatesLake KyogaRecent population bottleneckMitochondrial DNA markersDistinct genetic clustersPolymorphic microsatellite lociNile drainagePopulation size estimatesGenetic structurePopulation bottlenecksMain haplogroupsGenetic diversityMicrosatellite lociGenetic assignmentDNA markersPopulation structureMtDNA analysisGenetic admixtureSampling sitesGenetic unitsMost populationsTrypanosoma parasitesHuman African trypanosomiasis
2016
Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda
Gloria-Soria A, Dunn WA, Telleria EL, Evans BR, Okedi L, Echodu R, Warren WC, Montague MJ, Aksoy S, Caccone A. Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda. G3: Genes, Genomes, Genetics 2016, 6: 1573-1584. PMID: 27172181, PMCID: PMC4889654, DOI: 10.1534/g3.116.027235.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromosome MappingDNA, MitochondrialGene-Environment InteractionGenes, InsectGenetic LinkageGenetic VariationGenetics, PopulationGenome, InsectGenome-Wide Association StudyGenomicsGenotypeGeographyHigh-Throughput Nucleotide SequencingLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, Single NucleotideSelection, GeneticTsetse FliesUgandaConceptsSingle nucleotide polymorphismsLinkage disequilibriumExtent of LDLocal environmental adaptationGenome-wide variationDetection of lociOverall linkage disequilibriumInsect vectorsDifferent environmental conditionsWhole-genome sequencingPopulation genomicsStrong selectionAssociation analysisEnvironmental adaptationGenomic patternsPopulation dynamicsSignificant genetic associationUninfected fliesDisease transmissionSequence technologyAssociation studiesEnvironmental conditionsTsetse fliesHuman African trypanosomiasisRelevant phenotypesEvidence of temporal stability in allelic and mitochondrial haplotype diversity in populations of Glossina fuscipes fuscipes (Diptera: Glossinidae) in northern Uganda
Opiro R, Saarman NP, Echodu R, Opiyo EA, Dion K, Halyard A, Aksoy S, Caccone A. Evidence of temporal stability in allelic and mitochondrial haplotype diversity in populations of Glossina fuscipes fuscipes (Diptera: Glossinidae) in northern Uganda. Parasites & Vectors 2016, 9: 258. PMID: 27141947, PMCID: PMC4855780, DOI: 10.1186/s13071-016-1522-5.Peer-Reviewed Original Research
2015
Mitochondrial DNA sequence divergence and diversity of Glossina fuscipes fuscipes in the Lake Victoria basin of Uganda: implications for control
Kato AB, Hyseni C, Okedi LM, Ouma JO, Aksoy S, Caccone A, Masembe C. Mitochondrial DNA sequence divergence and diversity of Glossina fuscipes fuscipes in the Lake Victoria basin of Uganda: implications for control. Parasites & Vectors 2015, 8: 385. PMID: 26197892, PMCID: PMC4511262, DOI: 10.1186/s13071-015-0984-1.Peer-Reviewed Original ResearchConceptsMicrosatellite loci dataDemographic historyGenetic differentiationMicrosatellite dataLoci dataMitochondrial DNA (mtDNA) cytochrome oxidase II geneGenetic variationMitochondrial DNA sequence divergenceCytochrome oxidase II geneWeak genetic differentiationDNA sequence divergenceLake Victoria basinGenetic structuringGene flowDemographic testsGenetic divergenceMtDNA dataSequence divergenceGenetic diversityGeographic distanceVictoria basinGenetic analysisSampling sitesMutation ratePopulation clusters
2014
Analysis of Multiple Tsetse Fly Populations in Uganda Reveals Limited Diversity and Species-Specific Gut Microbiota
Aksoy E, Telleria EL, Echodu R, Wu Y, Okedi LM, Weiss BL, Aksoy S, Caccone A. Analysis of Multiple Tsetse Fly Populations in Uganda Reveals Limited Diversity and Species-Specific Gut Microbiota. Applied And Environmental Microbiology 2014, 80: 4301-4312. PMID: 24814785, PMCID: PMC4068677, DOI: 10.1128/aem.00079-14.Peer-Reviewed Original ResearchConceptsDifferent tsetse speciesRRNA gene clone librariesDifferent sympatric speciesGene clone librariesTsetse speciesLimited diversityQuantitative PCRV4 hypervariable regionGut microbiotaGut microbial abundanceSympatric speciesGut microbial diversityClone librariesMicrobial diversityMicrobial abundanceField fliesRRNA geneHost physiologyTsetse fly populationsDeep sequencingReduced diversityDifferent speciesFly populationsSpeciesWigglesworthia
2013
Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis
Echodu R, Sistrom M, Hyseni C, Enyaru J, Okedi L, Aksoy S, Caccone A. Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis. BioMed Research International 2013, 2013: 614721. PMID: 24199195, PMCID: PMC3807537, DOI: 10.1155/2013/614721.Peer-Reviewed Original ResearchConceptsSex-biased dispersalMicrosatellite DNA dataLack of admixtureAgents of humanVicariant barrierGenetic differentiationSouthern populationsDNA dataTrypanosoma bruceiCompetitive exclusionMain vector speciesSole vectorsContact zonePopulation clustersVector speciesEnvironmental conditionsTsetse fliesDistinct groupsAnimal trypanosomiasisHuman African trypanosomiasisGlossinaAfrican trypanosomiasisIntrogressionSympatryDispersalGlossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda
Aksoy S, Caccone A, Galvani AP, Okedi LM. Glossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda. Trends In Parasitology 2013, 29: 394-406. PMID: 23845311, PMCID: PMC3772539, DOI: 10.1016/j.pt.2013.06.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiodiversityDNA, MitochondrialFemaleGene FlowGenetics, PopulationHost-Pathogen InteractionsHumansInsect VectorsMaleMicrobiotaMicrosatellite RepeatsPest Control, BiologicalSymbiosisTrypanosoma brucei gambienseTrypanosoma brucei rhodesienseTrypanosomiasis, AfricanTsetse FliesUgandaConceptsOccurrence of polyandryGene flowPopulation geneticsPopulation structureGenetic applicationsHuman African trypanosomiasisMajor vectorMicrobiome compositionTsetse control programsTrypanosomiasis transmissionRecent spreadPotential rolePresent knowledgeGlossinaAfrican trypanosomiasisPolyandryHuman African trypanosomiasis transmissionInfection outcomesGeneticsFuscipesDiversityPopulationWolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics
Symula RE, Alam U, Brelsfoard C, Wu Y, Echodu R, Okedi LM, Aksoy S, Caccone A. Wolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics. BMC Ecology And Evolution 2013, 13: 31. PMID: 23384159, PMCID: PMC3574847, DOI: 10.1186/1471-2148-13-31.Peer-Reviewed Original ResearchConceptsMtDNA haplotypesDifferent WolbachiaGenetic diversityHost haplotypesSequence diversityMultiple WolbachiaArthropod host reproductionPopulation genetic differentiationComplex evolutionary dynamicsMitochondrial DNA haplotypesAssociation of WolbachiaLow reproductive outputHost population geneticsHost genetic diversityTsetse fliesMulti-locus sequence typingMost WolbachiaHost genetic compositionGenetic differentiationWolbachia evolutionWolbachia associationsHost reproductionDNA haplotypesMtDNA groupsΑ-proteobacteria
2012
The population structure of Glossina fuscipes fuscipes in the Lake Victoria basin in Uganda: implications for vector control
Hyseni C, Kato AB, Okedi LM, Masembe C, Ouma JO, Aksoy S, Caccone A. The population structure of Glossina fuscipes fuscipes in the Lake Victoria basin in Uganda: implications for vector control. Parasites & Vectors 2012, 5: 222. PMID: 23036153, PMCID: PMC3522534, DOI: 10.1186/1756-3305-5-222.Peer-Reviewed Original ResearchImplications of Microfauna-Host Interactions for Trypanosome Transmission Dynamics in Glossina fuscipes fuscipes in Uganda
Alam U, Hyseni C, Symula RE, Brelsfoard C, Wu Y, Kruglov O, Wang J, Echodu R, Alioni V, Okedi LM, Caccone A, Aksoy S. Implications of Microfauna-Host Interactions for Trypanosome Transmission Dynamics in Glossina fuscipes fuscipes in Uganda. Applied And Environmental Microbiology 2012, 78: 4627-4637. PMID: 22544247, PMCID: PMC3370491, DOI: 10.1128/aem.00806-12.Peer-Reviewed Original Research
2011
Influence of Host Phylogeographic Patterns and Incomplete Lineage Sorting on Within-Species Genetic Variability in Wigglesworthia Species, Obligate Symbionts of Tsetse Flies
Symula RE, Marpuri I, Bjornson RD, Okedi L, Beadell J, Alam U, Aksoy S, Caccone A. Influence of Host Phylogeographic Patterns and Incomplete Lineage Sorting on Within-Species Genetic Variability in Wigglesworthia Species, Obligate Symbionts of Tsetse Flies. Applied And Environmental Microbiology 2011, 77: 8400-8408. PMID: 21948847, PMCID: PMC3233068, DOI: 10.1128/aem.05688-11.Peer-Reviewed Original ResearchConceptsObligate symbiontsHost speciesGenetic variationMultiple host populationsIncongruence length difference testIncomplete lineage sortingHost-symbiont combinationsSingle host speciesPopulation-level processesIncomplete lineageLineage sortingPhylogeographic patternsSouthern lineagesSymbiont lineagesHost mtDNAWigglesworthia glossinidiaMolecular varianceBacterial symbiontsEvolutionary associationEvolutionary historySouthern populationsMitochondrial DNA haplogroupsVariable lociSymbiontsGenetic variabilityGenetic diversity and population structure of Glossina pallidipes in Uganda and western Kenya
Ouma JO, Beadell JS, Hyseni C, Okedi LM, Krafsur ES, Aksoy S, Caccone A. Genetic diversity and population structure of Glossina pallidipes in Uganda and western Kenya. Parasites & Vectors 2011, 4: 122. PMID: 21711519, PMCID: PMC3146932, DOI: 10.1186/1756-3305-4-122.Peer-Reviewed Original ResearchConceptsSex-biased dispersalGenetic diversityPopulation structureSource populationModern gene flowHigh genetic diversityMean genetic distanceAnalysis of mtDNAGene flowNuclear markersBayesian assignmentRelict populationsMtDNA haplotypesGenetic distanceMicrosatellite genotypesG. pallidipesGenetic variationPairwise relatednessMicrosatellite allelesMicrosatellite analysisDispersalSoutheastern UgandaFall populationPopulation comparisonsEffective control efforts