2021
A machine learning approach to integrating genetic and ecological data in tsetse flies (Glossina pallidipes) for spatially explicit vector control planning
Bishop AP, Amatulli G, Hyseni C, Pless E, Bateta R, Okeyo WA, Mireji PO, Okoth S, Malele I, Murilla G, Aksoy S, Caccone A, Saarman NP. A machine learning approach to integrating genetic and ecological data in tsetse flies (Glossina pallidipes) for spatially explicit vector control planning. Evolutionary Applications 2021, 14: 1762-1777. PMID: 34295362, PMCID: PMC8288027, DOI: 10.1111/eva.13237.Peer-Reviewed Original ResearchGenetic dataVectors of humanLake Victoria basinAnimal African trypanosomiasisGenetic connectivityVector-borne disease transmissionFuture monitoring effortsMicrosatellite lociHabitat useImportant environmental predictorsHabitat suitabilityFuture climatic changesDispersal patternsVictoria basinEcological dataEnvironmental predictorsVector systemTsetse fliesTsetse speciesClimatic shiftsFliesClimatic changesSampling sitesMonitoring effortsVector control
2018
Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya.
Okeyo WA, Saarman NP, Bateta R, Dion K, Mengual M, Mireji PO, Ouma C, Okoth S, Murilla G, Aksoy S, Caccone A. Genetic Differentiation of Glossina pallidipes Tsetse Flies in Southern Kenya. American Journal Of Tropical Medicine And Hygiene 2018, 99: 945-953. PMID: 30105964, PMCID: PMC6159567, DOI: 10.4269/ajtmh.18-0154.Peer-Reviewed Original ResearchConceptsGenetic differentiationPopulation dynamicsDispersal patternsTsetse fliesLow genetic diversityGreater genetic differentiationEvidence of admixtureWestern clusterEastern clusterRecent anthropogenic influencesAnimal African trypanosomiasisGenetic connectivityWestern relativesGenetic diversityMicrosatellite lociPopulation structureGenotypic dataWidespread signalDisease vectorsMajor vectorVector control effortsGreat Rift ValleyDemographic parametersFliesVector control programs
2017
Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
Okeyo WA, Saarman NP, Mengual M, Dion K, Bateta R, Mireji PO, Okoth S, Ouma JO, Ouma C, Ochieng J, Murilla G, Aksoy S, Caccone A. Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya. Parasites & Vectors 2017, 10: 471. PMID: 29017572, PMCID: PMC5635580, DOI: 10.1186/s13071-017-2415-y.Peer-Reviewed Original ResearchConceptsTemporal genetic differentiationGenetic diversityGenetic differentiationGenetic bottleneckGenetic variationNational ParkTemporal genetic variationExtensive control measuresAnimal African trypanosomiasisAllelic richnessPairwise FSTGenetic driftNe estimatesHabitat alterationPopulation geneticsTemporal differentiationMicrosatellite markersRuma National ParkClosest sampling sitesG. pallidipesMajor vectorTsetse fliesVector populationsDifferentiationDiversityMultiple evolutionary origins of Trypanosoma evansi in Kenya
Kamidi CM, Saarman NP, Dion K, Mireji PO, Ouma C, Murilla G, Aksoy S, Schnaufer A, Caccone A. Multiple evolutionary origins of Trypanosoma evansi in Kenya. PLOS Neglected Tropical Diseases 2017, 11: e0005895. PMID: 28880965, PMCID: PMC5605091, DOI: 10.1371/journal.pntd.0005895.Peer-Reviewed Original ResearchConceptsT. brucei strainsDifferent genetic backgroundsEvolutionary originGenetic diversityBrucei strainsMultiple evolutionary originsGenetic backgroundTsetse fly vectorT. evansiLethal human diseaseIndependent originsPolymorphic microsatellitesT. bruceiEvansi isolatesGenetic unitsHuman diseasesObligate linkBruceiEvansi strainsT. brucei bruceiTsetse fliesFly vectorsFliesDiversityBrucei brucei
2016
Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda
Gloria-Soria A, Dunn WA, Telleria EL, Evans BR, Okedi L, Echodu R, Warren WC, Montague MJ, Aksoy S, Caccone A. Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda. G3: Genes, Genomes, Genetics 2016, 6: 1573-1584. PMID: 27172181, PMCID: PMC4889654, DOI: 10.1534/g3.116.027235.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromosome MappingDNA, MitochondrialGene-Environment InteractionGenes, InsectGenetic LinkageGenetic VariationGenetics, PopulationGenome, InsectGenome-Wide Association StudyGenomicsGenotypeGeographyHigh-Throughput Nucleotide SequencingLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, Single NucleotideSelection, GeneticTsetse FliesUgandaConceptsSingle nucleotide polymorphismsLinkage disequilibriumExtent of LDLocal environmental adaptationGenome-wide variationDetection of lociOverall linkage disequilibriumInsect vectorsDifferent environmental conditionsWhole-genome sequencingPopulation genomicsStrong selectionAssociation analysisEnvironmental adaptationGenomic patternsPopulation dynamicsSignificant genetic associationUninfected fliesDisease transmissionSequence technologyAssociation studiesEnvironmental conditionsTsetse fliesHuman African trypanosomiasisRelevant phenotypes
2013
Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis
Echodu R, Sistrom M, Hyseni C, Enyaru J, Okedi L, Aksoy S, Caccone A. Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis. BioMed Research International 2013, 2013: 614721. PMID: 24199195, PMCID: PMC3807537, DOI: 10.1155/2013/614721.Peer-Reviewed Original ResearchConceptsSex-biased dispersalMicrosatellite DNA dataLack of admixtureAgents of humanVicariant barrierGenetic differentiationSouthern populationsDNA dataTrypanosoma bruceiCompetitive exclusionMain vector speciesSole vectorsContact zonePopulation clustersVector speciesEnvironmental conditionsTsetse fliesDistinct groupsAnimal trypanosomiasisHuman African trypanosomiasisGlossinaAfrican trypanosomiasisIntrogressionSympatryDispersalWolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics
Symula RE, Alam U, Brelsfoard C, Wu Y, Echodu R, Okedi LM, Aksoy S, Caccone A. Wolbachia association with the tsetse fly, Glossina fuscipes fuscipes, reveals high levels of genetic diversity and complex evolutionary dynamics. BMC Ecology And Evolution 2013, 13: 31. PMID: 23384159, PMCID: PMC3574847, DOI: 10.1186/1471-2148-13-31.Peer-Reviewed Original ResearchConceptsMtDNA haplotypesDifferent WolbachiaGenetic diversityHost haplotypesSequence diversityMultiple WolbachiaArthropod host reproductionPopulation genetic differentiationComplex evolutionary dynamicsMitochondrial DNA haplotypesAssociation of WolbachiaLow reproductive outputHost population geneticsHost genetic diversityTsetse fliesMulti-locus sequence typingMost WolbachiaHost genetic compositionGenetic differentiationWolbachia evolutionWolbachia associationsHost reproductionDNA haplotypesMtDNA groupsΑ-proteobacteria
2011
Temporal stability of Glossina fuscipes fuscipes populations in Uganda
Echodu R, Beadell JS, Okedi LM, Hyseni C, Aksoy S, Caccone A. Temporal stability of Glossina fuscipes fuscipes populations in Uganda. Parasites & Vectors 2011, 4: 19. PMID: 21320301, PMCID: PMC3045980, DOI: 10.1186/1756-3305-4-19.Peer-Reviewed Original ResearchConceptsEffective population size estimatesMitochondrial haplotype frequenciesEstimates of differentiationZone of contactJost's DESTPopulation size estimatesMitochondrial lociMating compatibilityMicrosatellite frequenciesMicrosatellite lociRiverine speciesPopulation dynamicsGenetic changesObserved seasonal fluctuationsHuman African trypanosomiasisMajor vectorSingle populationG. fuscipesTsetse fliesFuscipesAllele frequenciesHaplotype frequenciesLociSeasonal fluctuationsTime of sampling