2017
Genetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control
Opiro R, Saarman NP, Echodu R, Opiyo EA, Dion K, Halyard A, Dunn AW, Aksoy S, Caccone A. Genetic diversity and population structure of the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae) in Northern Uganda: Implications for vector control. PLOS Neglected Tropical Diseases 2017, 11: e0005485. PMID: 28453513, PMCID: PMC5425221, DOI: 10.1371/journal.pntd.0005485.Peer-Reviewed Original ResearchConceptsAnimal African trypanosomiasisGenetic clustersEffective population size estimatesLake KyogaRecent population bottleneckMitochondrial DNA markersDistinct genetic clustersPolymorphic microsatellite lociNile drainagePopulation size estimatesGenetic structurePopulation bottlenecksMain haplogroupsGenetic diversityMicrosatellite lociGenetic assignmentDNA markersPopulation structureMtDNA analysisGenetic admixtureSampling sitesGenetic unitsMost populationsTrypanosoma parasitesHuman African trypanosomiasis
2016
Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda
Gloria-Soria A, Dunn WA, Telleria EL, Evans BR, Okedi L, Echodu R, Warren WC, Montague MJ, Aksoy S, Caccone A. Patterns of Genome-Wide Variation in Glossina fuscipes fuscipes Tsetse Flies from Uganda. G3: Genes, Genomes, Genetics 2016, 6: 1573-1584. PMID: 27172181, PMCID: PMC4889654, DOI: 10.1534/g3.116.027235.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromosome MappingDNA, MitochondrialGene-Environment InteractionGenes, InsectGenetic LinkageGenetic VariationGenetics, PopulationGenome, InsectGenome-Wide Association StudyGenomicsGenotypeGeographyHigh-Throughput Nucleotide SequencingLinkage DisequilibriumMicrosatellite RepeatsPolymorphism, Single NucleotideSelection, GeneticTsetse FliesUgandaConceptsSingle nucleotide polymorphismsLinkage disequilibriumExtent of LDLocal environmental adaptationGenome-wide variationDetection of lociOverall linkage disequilibriumInsect vectorsDifferent environmental conditionsWhole-genome sequencingPopulation genomicsStrong selectionAssociation analysisEnvironmental adaptationGenomic patternsPopulation dynamicsSignificant genetic associationUninfected fliesDisease transmissionSequence technologyAssociation studiesEnvironmental conditionsTsetse fliesHuman African trypanosomiasisRelevant phenotypesWhole genome sequencing shows sleeping sickness relapse is due to parasite regrowth and not reinfection
Richardson JB, Evans B, Pyana PP, Van Reet N, Sistrom M, Büscher P, Aksoy S, Caccone A. Whole genome sequencing shows sleeping sickness relapse is due to parasite regrowth and not reinfection. Evolutionary Applications 2016, 9: 381-393. PMID: 26834831, PMCID: PMC4721075, DOI: 10.1111/eva.12338.Peer-Reviewed Original ResearchSame patientHuman African trypanosomiasisHigh incidencePatientsTrypanosoma brucei gambienseHAT casesDrug resistanceDisease controlRelapseSingle nucleotide polymorphismsBrucei gambienseWhole-genome sequencingAfrican trypanosomiasisPathogenic levelsSaharan AfricaNucleotide polymorphismsOriginal strainRecent studiesTreatmentPromising new setFunctional studiesNovel insightsReinfectionFuture functional studiesNew strain
2014
Comparative Genomics Reveals Multiple Genetic Backgrounds of Human Pathogenicity in the Trypanosoma brucei Complex
Sistrom M, Evans B, Bjornson R, Gibson W, Balmer O, Mäser P, Aksoy S, Caccone A. Comparative Genomics Reveals Multiple Genetic Backgrounds of Human Pathogenicity in the Trypanosoma brucei Complex. Genome Biology And Evolution 2014, 6: 2811-2819. PMID: 25287146, PMCID: PMC4224348, DOI: 10.1093/gbe/evu222.Peer-Reviewed Original ResearchConceptsT. brucei complexGenomic variationLife historyVariable life historiesAverage linkage disequilibriumTrypanosoma brucei complexGenome-wide studiesMeiotic reciprocal recombinationWhole genome sequencesMultiple genetic backgroundsHuman pathogenicityNumber of subspeciesHuman-infective parasiteNuclear genomeComparative genomicsChromosome sizeEukaryotic parasitesGenomic regionsHuman African trypanosomiasisRegulatory genesTaxonomic designationsDiverse hostsSingle nucleotide polymorphismsGenetic variationReciprocal recombination
2013
Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis
Echodu R, Sistrom M, Hyseni C, Enyaru J, Okedi L, Aksoy S, Caccone A. Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis. BioMed Research International 2013, 2013: 614721. PMID: 24199195, PMCID: PMC3807537, DOI: 10.1155/2013/614721.Peer-Reviewed Original ResearchConceptsSex-biased dispersalMicrosatellite DNA dataLack of admixtureAgents of humanVicariant barrierGenetic differentiationSouthern populationsDNA dataTrypanosoma bruceiCompetitive exclusionMain vector speciesSole vectorsContact zonePopulation clustersVector speciesEnvironmental conditionsTsetse fliesDistinct groupsAnimal trypanosomiasisHuman African trypanosomiasisGlossinaAfrican trypanosomiasisIntrogressionSympatryDispersalGlossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda
Aksoy S, Caccone A, Galvani AP, Okedi LM. Glossina fuscipes populations provide insights for human African trypanosomiasis transmission in Uganda. Trends In Parasitology 2013, 29: 394-406. PMID: 23845311, PMCID: PMC3772539, DOI: 10.1016/j.pt.2013.06.005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiodiversityDNA, MitochondrialFemaleGene FlowGenetics, PopulationHost-Pathogen InteractionsHumansInsect VectorsMaleMicrobiotaMicrosatellite RepeatsPest Control, BiologicalSymbiosisTrypanosoma brucei gambienseTrypanosoma brucei rhodesienseTrypanosomiasis, AfricanTsetse FliesUgandaConceptsOccurrence of polyandryGene flowPopulation geneticsPopulation structureGenetic applicationsHuman African trypanosomiasisMajor vectorMicrobiome compositionTsetse control programsTrypanosomiasis transmissionRecent spreadPotential rolePresent knowledgeGlossinaAfrican trypanosomiasisPolyandryHuman African trypanosomiasis transmissionInfection outcomesGeneticsFuscipesDiversityPopulation
2012
Trypanosoma brucei gambiense Group 1 Is Distinguished by a Unique Amino Acid Substitution in the HpHb Receptor Implicated in Human Serum Resistance
Symula RE, Beadell JS, Sistrom M, Agbebakun K, Balmer O, Gibson W, Aksoy S, Caccone A. Trypanosoma brucei gambiense Group 1 Is Distinguished by a Unique Amino Acid Substitution in the HpHb Receptor Implicated in Human Serum Resistance. PLOS Neglected Tropical Diseases 2012, 6: e1728. PMID: 22802982, PMCID: PMC3393672, DOI: 10.1371/journal.pntd.0001728.Peer-Reviewed Original ResearchConceptsTrypanosome lytic factorHuman serum resistanceGroup 2Group 1Serum resistanceHaptoglobin-hemoglobin receptorHuman serumHuman African trypanosomiasisInnate immunityTrypanosoma brucei rhodesienseUnique amino acid substitutionsAltered activityLytic factorCausative agentLytic activityHemoglobin receptorTLF-1African trypanosomiasisBrucei rhodesienseSerumReceptorsAmino acid substitutionsFurther evidenceHpHbRTBR
2011
Temporal stability of Glossina fuscipes fuscipes populations in Uganda
Echodu R, Beadell JS, Okedi LM, Hyseni C, Aksoy S, Caccone A. Temporal stability of Glossina fuscipes fuscipes populations in Uganda. Parasites & Vectors 2011, 4: 19. PMID: 21320301, PMCID: PMC3045980, DOI: 10.1186/1756-3305-4-19.Peer-Reviewed Original ResearchConceptsEffective population size estimatesMitochondrial haplotype frequenciesEstimates of differentiationZone of contactJost's DESTPopulation size estimatesMitochondrial lociMating compatibilityMicrosatellite frequenciesMicrosatellite lociRiverine speciesPopulation dynamicsGenetic changesObserved seasonal fluctuationsHuman African trypanosomiasisMajor vectorSingle populationG. fuscipesTsetse fliesFuscipesAllele frequenciesHaplotype frequenciesLociSeasonal fluctuationsTime of sampling
2008
Multiple-strain infections of Trypanosoma brucei across Africa
Balmer O, Caccone A. Multiple-strain infections of Trypanosoma brucei across Africa. Acta Tropica 2008, 107: 275-279. PMID: 18671933, PMCID: PMC2582348, DOI: 10.1016/j.actatropica.2008.06.006.Peer-Reviewed Original Research