2016
IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses
Lee CM, He CH, Nour AM, Zhou Y, Ma B, Park JW, Kim KH, Cruz CD, Sharma L, Nasr ML, Modis Y, Lee CG, Elias JA. IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses. Nature Communications 2016, 7: 12752. PMID: 27629921, PMCID: PMC5027616, DOI: 10.1038/ncomms12752.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell Line, TumorChitinase-3-Like Protein 1Heparin-binding EGF-like Growth FactorHumansInterleukin-13 Receptor alpha2 SubunitLung InjuryLung NeoplasmsMAP Kinase Signaling SystemMelanoma, ExperimentalMembrane ProteinsMice, Inbred C57BLNeoplasm MetastasisProto-Oncogene Proteins c-aktTransforming Growth Factor beta1Two-Hybrid System TechniquesWnt Signaling PathwayConceptsNull mutationPKB/Akt activationBimolecular fluorescence complementationDirect physical interactionMAPK/ERKHB-EGF productionFluorescence complementationTwo-hybridMembrane proteinsAkt activationDecoy functionPhysical interactionIL-13Rα2Effect of CHI3L1Chitinase 3Critical roleSignalingMutationsRecent studiesCHI3L1ComplementationTGF-β1YeastMelanoma metastasesERK
2014
Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry
Sharma L, Wu W, Dholakiya SL, Gorasiya S, Wu J, Sitapara R, Patel V, Wang M, Zur M, Reddy S, Siegelaub N, Bamba K, Barile FA, Mantell LL. Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry. Methods In Molecular Biology 2014, 1172: 137-145. PMID: 24908301, DOI: 10.1007/978-1-4939-0928-5_12.ChaptersMeSH KeywordsAnimalsCell LineFlow CytometryFluorescein-5-isothiocyanateFluorescent DyesGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHMGB1 ProteinInterleukin-1betaMacrophagesMiceMicroscopy, FluorescenceMicrospheresPhagocytosisTransforming Growth Factor beta1Tumor Necrosis Factor-alphaConceptsCultured macrophagesHigh mobility group box 1Individual cellsInnate immune systemFluorescence microscopyNumerous diseasesPhagocytic processFluorescence microscopePhagocytic functionPhagocytic activityCell debrisPhagocytosisFlow cytometryMobility group box 1PhagocytesGroup box 1GM-CSFMacrophagesImmune systemTGF-β1Different cytokinesFlow cytometerIL-1βEngulfmentHomeostasis