2024
Streamlined Nucleus Isolation Protocol for Cryopreserved Mouse Lung Captures Non-immune Cell Types by Single-nucleus RNA Sequencing
Tolman N, Tabary M, Sharma L, Dela Cruz C, Alder J, Lee J, Chen K, Bain W. Streamlined Nucleus Isolation Protocol for Cryopreserved Mouse Lung Captures Non-immune Cell Types by Single-nucleus RNA Sequencing. 2024, a3315-a3315. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a3315.Peer-Reviewed Original Research
2023
Integrated Analysis of Tracheobronchial Fluid from Before and After Cardiopulmonary Bypass Reveals Activation of the Integrated Stress Response and Altered Pulmonary Microvascular Permeability
Habet V, Li N, Qi J, Peng G, Charkoftaki G, Vasiliou V, Sharma L, Pober J, Dela Cruz C, Yan X, Pierce R. Integrated Analysis of Tracheobronchial Fluid from Before and After Cardiopulmonary Bypass Reveals Activation of the Integrated Stress Response and Altered Pulmonary Microvascular Permeability. The Yale Journal Of Biology And Medicine 2023, 96: 23-42. PMID: 37009190, PMCID: PMC10052603, DOI: 10.59249/kfyz8002.Peer-Reviewed Original ResearchConceptsIntegrated stress responseProteomic analysisTranscriptional activityStress responseSingle-cell RNA sequencingCell RNA sequencingHuman pulmonary microvascular endothelial cellsMulti-omics approachCell type annotationRespiratory transport chainUnbiased proteomic analysisUpregulation of proteinsIngenuity Pathway AnalysisCardiopulmonary bypassCell clusteringProtective cellular responseFunctional cellular assaysDistinct cell populationsDEG analysisCellular phenotypesRNA sequencingPathway analysisTransport chainCellular responsesImmune cells